We narrowed to 359 results for: Nov
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TypeBlog Post...career that could simply cease to exist. But then November hit and panic ensued as “alternative facts” became...
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Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post...many great insights, it can be overwhelming to a novice microscopist. Hopefully this brief synopsis can... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...methods include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid electroporation... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...with BioRender.com. Although it was a huge innovation, there was a limitation to this approach. CBEs... -
Antibody Validation for Flow Cytometry
TypeBlog Post... the protein target will depend on the protein turnover rate. If you need a more permanent knockdown, ... -
Deep Dive: qPCR
TypeBlog Post...between your experimental values and your controls. ANOVA, t-test, multiple regression analysis, and non-parametric... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post... Guschin, D., Fong, L. K., Vu, B. J., Meng, X., Urnov, F. D., Rebar, E. J., Gregory, P. D., Zhang, H. ... -
Modular Cloning Guide
TypeGuide...cloning toolbox for plants. ACS Synth Biol . 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. PMID: 24933124... Yeast Expression Brigitte Gasser , Diethard Mattanovich , and Michael Sauer 62 plasmids for advanced ...Genome Engineering, TALEN Daniel Voytas and Adam Bogdanove Plasmid toolkit to efficiently assemble TALEN ...Golden Gate TALEN and TAL Effector Kit (Voytas and Bogdanove). Platinum Gate TALEN Kit Genome Engineering, ... -
Adeno-associated virus (AAV) Guide
TypeGuide...Adeno-Associated Virus versus Adenovirus Adenoviruses (AdV) are part of the Adenoviridae family, are medium-sized...presence of adenoviral genes E1, E4, E2a and VA for replication. For more information about adenovirus, read...deficient and depend on other viruses such as adenovirus or herpes simplex virus to replicate in the host...family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they got their name...read our Adenoviral Vector Guide . Recombinant AAV Vectors Wild-type AAVs have been modified by researchers...of viral capsids. Helper plasmid — containing adenoviral genes not present in the AAV genome required ...for rAAV vector production needs to express the adenovirus E1 gene, which is crucial for efficient AAV replication... -
CRISPR Guide
TypeGuide...Seegers, C. L., Bollen, S., Jore, M. M., Semenova, E., Severinov, K., De Vos, W. M., Dame, R. T., De Vries...control both when and where genome editing happens. CASANOVA , developed by Dominik Niopek’s lab , is a way...It has also been used extensively to screen for novel genes that regulate known phenotypes, including ... maintained by the Jennifer Doudna lab at the Innovative Genomics Institute, University of California,... 35301321 Edraki, A., Mir, A., Ibraheim, R., Gainetdinov, I., Yoon, Y., Song, C., Cao, Y., Gallant, J....30403660 Sanson, K. R., Hanna, R. E., Hegde, M., Donovan, K. F., Strand, C., Sullender, M. E., Vaimberg,..., Gootenberg, J. S., Wu, W. Y., Scott, D. A., Severinov, K., Van Der Oost, J., & Zhang, F. (2017). Multiplex... -
Optogenetics Guide
TypeGuide...by scientists through both the identification of novel ChRs from other algal species and the development...different effector domains of proteins to generate novel engineered light-controlled molecules. ~450–495 ...22179551 Saunders A, Johnson CA, Sabatini BL 2012. Novel recombinant adeno-associated viruses for Cre activated... -
Addgene's eBook Collection
TypeGuide...Science Career Guide November 2018 | First Edition About Science Career Guide November 2018 | First... -
Gamma-Retroviral Vector Guide
TypeGuide...integration marks cell Type-Specific cancer genes: a novel profiling tool in cancer genomics. PLoS ONE , 11...pseudotyping of different retroviral vectors using a novel, CoDOn-Optimized gene for chimeric GALV envelope... -
Science Guides
TypeGuide...including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus Read More... -
Chemogenetics Guide
TypeGuide...2012). Design and functional characterization of a novel, arrestin-biased designer G protein-coupled receptor... -
Plan Your Experiment
TypeGuide... B., Chen, J., Fulco, C. P., Jerby-Arnon, L., Marjanovic, N. D., Dionne, D., Burks, T., Raychowdhury, ... -
Lentiviral Vector Guide
TypeGuide..., Hahn, W. C., Sharp, P. A., Weinberg, R. A., & Novina, C. D. (2003). Lentivirus-delivered stable gene... -
Molecular Biology Reference
TypeGuide...particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that can infect your target cells ... -
Antibody Guide
TypeGuide...sdAb. sdAbs can have weak signals due to their monovalent nature. Darpins - Darpins are genetically engineered...