We narrowed to 564 results for: PAC;

...of interest that are found in the extracellular space. The targeted epitopes can be motifs within transmembrane...Proliferation dyes can visualize proliferative capacity and speed of cells. (a) With each division half...

...application can benefit many others and accelerate the pace of research. We at Addgene hope to see the problem...PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. ...

...sample migrate through the stacking gel at the same pace and enter the resolving gel at the same time where...popular due to its durability and high binding capacity. In addition, PVDF membranes can be repeatedly...

...modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression or silencing...particularly large protein, and this fusion allows for packaging into viral vectors and targeted delivery into ...

...Plus, the tools used here (also available as AAV Packaged on Request) will be useful for related studies...transfer increases the metabolic and metastatic capacity of cancer cells. This is certainly just the beginning...

...needed to replicate inside a host cell but with space to insert your genetic cargo of choice. After hearing...taken them for a test drive! We validated viral packaging and strong expression after inserting EGFP and...

...are composed. Fear not – the state machine design space is highly degenerate and often times there are multiple... et al. "Permanent genetic memory with> 1-byte capacity." Nature methods 11.12 (2014): 1261-1266. PubMed...

...Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated) with no known...certain genes, such as those that have negative impacts on fitness. One consideration when making a transgenic...

... much as possible and the ones that are tightly packed with seals. Use disposable pipettes to avoid cross-contamination...be overcrowded (filled no more than 60% of its capacity) which can cause mycoplasma infection from one...

... a good story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold...LumiFluor reporter constructs were designed to be packaged into a retrovirus for infection of host cells....

... epigenetic modification, bulges) that may also impact off-target edits. As a result, only sequencing ...algorithms may not provide a full picture of the impact of CRISPR editing in the model cell line or organism...

...other side, are too long for rAAVs, which can only package sequences shorter than 5 kb (Hirsch et al., 2016...Delivering transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological...

...populations that are the most likely to be positively impacted by the trial. During clinical trials, expansion... strategies that synergize with these positive impacts on the immune system will likely have better treatment...

...implications for our public policies—many of which impact reproductive rights and justice. Whether it’s used...interests. Our marches across the world have had an impact already, gaining additional funding and the eyes...

...distance in the placement of plasmid elements can impact expression levels and consequently convolute the... different lentiviral plasmid backbone elements impact viral titer and to compare different Cas enzymes...

... formation slightly. Designing the sgRNA with a spacer that only binds the edited strand, as in the PE3b...Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell, 186(18), 3983-4002...

...multiplex your reference genes to save on plate space. For many cell lines and tissue types, appropriate...your reference gene set can save significant plate space and may be worth the initial effort of setting it...

...combination of all 11 att pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable...compartment (e.g. the plasma membrane, the extracellular space, or the lysosomes) can take many paths. Understanding... to other viral systems due to its large cargo capacity, minimal cytotoxicity, and broad host-tropism....

... Gateway-Compatible Toolbox Podcast Segment The impact of KLF4 N-terminal variants on iPSC generation ... expression levels; this altered stoichiometry impacted reprogramming and global gene expression patterns...

...each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence just downstream...