...other side, are too long for rAAVs, which can only package sequences shorter than 5 kb (Hirsch et al., 2016...Delivering transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological...

... epigenetic modification, bulges) that may also impact off-target edits. As a result, only sequencing ...algorithms may not provide a full picture of the impact of CRISPR editing in the model cell line or organism...

... much as possible and the ones that are tightly packed with seals. Use disposable pipettes to avoid cross-contamination...be overcrowded (filled no more than 60% of its capacity) which can cause mycoplasma infection from one...

...implications for our public policies—many of which impact reproductive rights and justice. Whether it’s used...interests. Our marches across the world have had an impact already, gaining additional funding and the eyes...

...populations that are the most likely to be positively impacted by the trial. During clinical trials, expansion... strategies that synergize with these positive impacts on the immune system will likely have better treatment...

...distance in the placement of plasmid elements can impact expression levels and consequently convolute the... different lentiviral plasmid backbone elements impact viral titer and to compare different Cas enzymes...

... formation slightly. Designing the sgRNA with a spacer that only binds the edited strand, as in the PE3b...Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell, 186(18), 3983-4002...

...Dynamics in the Mitochondrial Matrix and Intermembrane Space with the GZnP2 Sensor. ACS Chem Biol. 2018 Jul 20...levels for imaging in live cells Fourth-generation epac-based FRET sensors for cAMP feature exceptional ...

...multiplex your reference genes to save on plate space. For many cell lines and tissue types, appropriate...your reference gene set can save significant plate space and may be worth the initial effort of setting it...

...combination of all 11 att pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable...compartment (e.g. the plasma membrane, the extracellular space, or the lysosomes) can take many paths. Understanding... to other viral systems due to its large cargo capacity, minimal cytotoxicity, and broad host-tropism....

... Gateway-Compatible Toolbox Podcast Segment The impact of KLF4 N-terminal variants on iPSC generation ... expression levels; this altered stoichiometry impacted reprogramming and global gene expression patterns...

...each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence just downstream...

...CRISPR-associated transposases (CASTs) combine the large-scale capacity of transposases (Tns) with the precise DNA-targeting...

...Kits Kits are groups of plasmids that have been packaged together because they are intended for use as ...

...research output, RESOLUTE expects to accelerate the pace of research in the field of SLCs to the global benefit...

...course-based undergraduate research experiences (CURE) impacts student learning and attitudinal outcomes: A study...

...Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from The Michael...

...specific vector to use depends on the length of the spacer sequence between the half-sites (see Nuclease Expression...

... survival and cytoskeletal organization. Cancer Impact Enhanced signaling through either mTORC1 or mTORC2...

...with a typical gRNA, the pegRNA is designed with a spacer that binds to a specific genomic DNA locus and ...