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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 354 results for: Plat;

Showing: 341 - 354 of 354 results

  1. Protocol - How to Design Primers

    Type
    Protocol
    ...correspond to sequences on the template molecule (must be complementary to template strand). However, primers...to design primers that are complementary to the template region of DNA. They are synthesized chemically...primers do not need to correspond to the template strand completely; it is essential, however, that the 3’...end of the primer corresponds completely to the template DNA strand so elongation can proceed. Usually ...

  2. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...your LB agar plates are not fresh, you should streak your bacteria onto a new LB agar plate before growing... Streaking and Isolating Bacteria on an LB Agar Plate Creating Bacterial Glycerol Stocks for Long-term...commonly used to culture bacteria in the lab. LB agar plates are frequently used to isolate individual (clonal...toothpick, select a single colony from your LB agar plate . Drop the tip or toothpick into the liquid LB +...

  3. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...new window) Link to Video Making LB Agar Plates Create plates to culture bacteria in the lab Watch the...Video! Over-Agar Antibiotic Plating Quickly add antibiotic to a pre-poured plate Watch the Video! Streaking...Bacteria Isolate single bacterial colonies on an agar plate Watch the Video! Inoculating a Liquid Bacterial ...

  4. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...with shaking platform set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents...water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved. Note: ...flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm. Pro-Tip Do not use cells that ...

  5. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...days. Single cells were then plated in individual wells of a 96-well plate and left undisturbed for 13 ... well (each well gets one dilution, so a 6-well plate will hold 5 dilutions plus one 'no virus' control...

  6. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...competent cells and retransform. Bacteria on an LB agar plate can be stored at 4°C for a few weeks. However, if...stock unthaw! Streak the bacteria onto an LB agar plate . Grow your bacteria overnight at the appropriate...

  7. Colony Formation Titering Assay

    Type
    Protocol
    ...(μL) Volume of Virus Added to Plate (μL) Volume of Cells Added to Plate (μL) Final Viral Dilution 1:10...

  8. AAV Production in HEK293 Cells

    Type
    Protocol
    ...controller Hazardous waste container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex...reach 100% confluence (80–90% is ideal). Pass and plate the cells the day before the transfection. Thaw ...

  11. Ligation Independent Cloning

    Type
    Protocol
    ...sequence is used, plus a minimum of 18 bp of your template sequence. 5' and 3' primers will have different...

  12. Pipetting Protocol

    Type
    Protocol
    ...liquid into different tubes or into wells of a microplate, you may consider using a multichannel pipette...
Showing: 341 - 354 of 354 results