... Werner S, Marillonnet S. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PubMed... with a knockout and expression construct on the same piece of DNA. We do a lot of protein-protein interaction...

...backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene...backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors then used GMAP assembly...engineering (MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand...number of tandemly expressed gRNAs respectively) and 2) the size of the deletion. Smaller deletions generally...population within a given tissue rather than a mixed sample. TRIBE was developed in Drosophila, and further...

...Nature 339 (1989). 487–488. PubMed PMID: 2725683. 2. Miller, C.J., Kassem H.S., Pepper S.D., Hey Y., Ward.... Know your target cells Labs tend to follow the same general procedure for lentiviral infections, produce...but also between different lots of serum from the same source. Labs that routinely produce lentivirus may...

...  Figure 2: Examples of simultaneous multiplex IF labeling of rat brain samples from the James ...presence of several biomarkers together in the same tissue sample predicts a certain cancer and want to look...you will need to start with a fixed sample. Depending on the sample type, this might be frozen or formalin-fixed...al, 2022 for a sample protocol.)  Simultaneous IF Depending on your needs, your sample, and targets, you...known to be abundant in your samples (with a primary antibody that has the same species and isotype as the...set of samples where you label only one target in each. Compare these individually-labeled samples with ...referred to as immunocytochemistry (ICC) when the sample is cultured cells or immunohistochemistry (IHC)...

...of 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), a fluorophore modified from DsRed. DFHO...orange fluorescent aptamers Corn was described in the same study as Red Broccoli and Orange Broccoli, two more...PMC5679246. Strack, Rita L., Matthew D. Disney, and Samie R. Jaffrey. "A superfolding Spinach2 reveals the...

..., can protect cryo-EM samples from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue...protect fragile samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM samples can be damaged...and structure determination LEA proteins protect samples during cryo-EM grid freezing Engineered and evolved...antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment and structure determination By Mike ...enrichment streamlines the purification and minimizes sample loss while also supporting imaging, making it easy...cryo-EM grid.     Figure 1: MagIC-cryo-EM for sample enrichment and structure determination. A) Schematic...beads for scarce macromolecules in heterogeneous samples. bioRxiv 2024.01.21.576499; doi: https://doi.org...

...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...function like your average AAVs — and have many of the same restrictions, like limited cargo space (~4.7 kb)...systems, which use two or more AAVs to transduce the same cell, can accommodate larger, modular genetic payloads...efficient AAV-PHP.eB, developed and deposited by the same lab. AAV-PHP.eB is the most commonly requested systemic...off-target organs. AAV.CAP-B22 was developed through the same process as AAV.CAP-B10 and shows high transduction...

...systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR type I-E. Created with...using Cas9, two or more gRNAs must be used along the same chromosome. During DNA repair, there is a chance...

...of concept for further development. Figure 2: Mechanisms of deaminase-based adenine transversion...instead of human AAG (remember, MPG and AAG are the same protein!) (Chen et al., 2024). They called their...different teams succeeded with this approach around the same time (He et al., 2024; Tong et al., 2024; Ye et ...hypoxanthine from DNA, creating an abasic site. The same protein (or its homologs) has more than one name...

...that view (Callahan et al., 2022).     Fig. 2: Students in an MDH CURE replaced select residues ...happened in the lab, and he wanted to provide the same kinds of opportunities for his students. But he ...

...would help them grasp some of the key concepts (Fig. 2). One thing I will likely change for next time is ...essentially background slides getting everyone on the same page in terms of the vocabulary--DNA, protein, plasmid...

... Methods 2009; 6, 343-345. PubMed PMID: 19363495. 2. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey... mix of enzymes is that they can all work at the same temperature, so the entire reaction takes an hour... to complete at 50 °C. After an hour or so, the sample is immediately ready to transform into competent... generate wild type and mutant constructs at the same time, rather than sequentially.   How does Gibson...

...mazF gene and a gene for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct... introduce multiple genetic modifications in the same strain. MazF expression is under control of the ...

...PMID: 18478103. PubMed Central PMCID: PMC2364656. 2. Dittmar, Kimberly A., et al. "Selective charging ...producing additional tRNAs is that you can use the same expression system for many different genes without...

...) or the American Chemical Society (ACS) are just 2 examples of organizations that hold large, annual ...stand out from the hundreds of others that did the same thing? Make an effort to meet people. Attend smaller...

...the necessary steps to perform:      Fig. 2: Quick-stepping to a FASTA file   With our outline...knew we could rely on the containers to produce the same output regardless of who or which computer ran the...

...Single-chain variable fragment (ScFv) Figure 2: Comparison between the IgG antibody and scFv. ... exact amount of protein in your sample, you’ll compare your samples to a standard curve. This curve is...the known samples is used to calculate the concentration of protein in the experimental samples. Get more...multiple proteins on the same cell simultaneously as well as compare between samples. Learn more about flow...of different isotypes can be used together in the same experiment because the reagents used to detect the... population of antibodies that all recognize the same epitope of the target protein. Most commonly these...expression in cell lysates, whole cells, or tissue samples. Many experiments that use antibodies use a primary...

...positive cells (Martin et al., 2019). Figure 2: Editing efficiencies using base editing reporters...by canonical base excision repair enzymes. At the same time Cas9n cleaves the opposing DNA strain which...

...to the break room for a sip of water.  Fig 2: Blugene likes to spark creativity in out on the open...you and you will arrive at unique solutions to the same problem. If your lab/company/team is still working...

...gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) General protein expression...-deficient; allows for cloning and expression in same strain F- recA1 hsdR(rK12- mK12+) (DE3) (RifR) ...