We narrowed to 767 results for: SCR

...encoded on RNA. The genome includes a reverse transcriptase and an integrase to insert the viral genome ...plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression...cloning and gene expression. Select with puromycin or screen for GFP. David Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...plasmid 12282 for the addition of WRE, a post-transcriptional regulatory element that increases export to...Gamma-Retroviral Packaging Plasmids ID Plasmid Description PI 8449 pUMVC Contains MoMLV gag and pol . Use...Gamma-Retroviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended for the FELIX...

...supporting the tools in our collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub... to learn more about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi,...

...communication between all members of a team. As described here in a 2012 Intuit blog post, a study coming...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...

...expression plasmids. Large collections or kits are described below and not included in this table. For a more...search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes...strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in C. elegans via ...genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing...also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery of Custom Genetic...C. elegans and other nematodes. Wormbuilder - Describes methods to engineer the genome of the genetic ...

...mutations The researchers used the collection to screen individual perturbations in vitro using Ba/F3 and...targeted therapeutic interventions for cancer. This screening platform is an incredible boost to the cancer ...

... interacting partners and perform whole animal screens for small molecules that block the neurodegenerative...and pathways to target in future small molecule screens. He plans to work with a partner at IRCCS Besta...

...released the MORF collection, a comprehensive transcription factor library, in lentiviral vectors. At 3500...conduct robust investigations into the human transcriptome. The MORF library is only one of 22 libraries...

...from the Zenodo dataset.     Figure 2: Screenshot showing reagent entries with associated metadata...others in the IBEX community.   Figure 3: Screenshot showing recent topics in the IBEX discussion ...

...updated! Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene...

...The system consists of dCas9, the mammalian transcriptional repressor Mxi1, and an optogenetic tool that...editing. Using several rapid and cost-effective screening methods, David Liu’s lab identified and validated...

... greater than any fluorescent protein earlier described. “Until our proteins, there were lots of fluorescent...illumination with far-red light. As the researchers described in Nature Communications last year, “The capability...

...These sensors generally follow the same pattern described above - a sensing domain induces changes in a ... such as protein or DNA binding, enzyme or transcriptional activation, conformational changes, translation...

...better. More transcription factor binding sites in a promoter lead to higher transcriptional activation....

...showing the effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists...plasmids are available as a collection, facilitating screening across the transducerome, to gain a more complete...

... that everyone can access data and drafts of manuscripts and grant applications in real-time so they can...figure out are timelines for experiments and manuscripts, who is responsible for which parts of the project...

...called JEDI-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21... 1: Summary of the approach and outcomes of the screen for improved genetically-encoded voltage indicators...

...Lentiviral vector design. B) Strategy for leak-free transcriptional control. C) Iterative transduction workflow...of biosensors could be used in high throughput screening for agrochemical compounds that have the potential...

... and variable spacer sequences. The array is transcribed and processed into crRNAs, each including one...from Addgene!   RNA crRNA: “CRISPR RNAs” are transcribed from the CRISPR array and include a spacer and...

...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...

...find the vector that fits your experiments, I've described below some of the most frequently requested vectors... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of...