We narrowed to 763 results for: SCR

...allows you to fuse proteins of interest to transcriptional regulatory elements that will turn on gene ...and an engineered strain for a B2H system used to screen the activity of arrays of zinc finger nucleases...Propionate Escherichia coli Jay Keasling 113634 pSCrhaB2 PrhaB Rhamnose Burkholderia cenocepacia Miguel...nm) Escherichia coli Christopher Voigt 164226 pSCrhaB2plus PrhaBAD Rhamnose Burkholderia sp. Silvia Cardona...selection of plasmids using tetracycline-regulated transcriptional activation or repression. The BIOFAB plasmid...that element. When this activation requires a transcription factor to bind to a small molecule, the reporter...DNA-binding specificity of proteins such as transcription factors. Addgene Blog Luciferase Technologies...

...mutations The researchers used the collection to screen individual perturbations in vitro using Ba/F3 and...targeted therapeutic interventions for cancer. This screening platform is an incredible boost to the cancer ...

...The system consists of dCas9, the mammalian transcriptional repressor Mxi1, and an optogenetic tool that...editing. Using several rapid and cost-effective screening methods, David Liu’s lab identified and validated...

... interacting partners and perform whole animal screens for small molecules that block the neurodegenerative...and pathways to target in future small molecule screens. He plans to work with a partner at IRCCS Besta...

...released the MORF collection, a comprehensive transcription factor library, in lentiviral vectors. At 3500...conduct robust investigations into the human transcriptome. The MORF library is only one of 22 libraries...

...from the Zenodo dataset.     Figure 2: Screenshot showing reagent entries with associated metadata...others in the IBEX community.   Figure 3: Screenshot showing recent topics in the IBEX discussion ...

...updated! Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene...

... greater than any fluorescent protein earlier described. “Until our proteins, there were lots of fluorescent...illumination with far-red light. As the researchers described in Nature Communications last year, “The capability...

...showing the effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists...plasmids are available as a collection, facilitating screening across the transducerome, to gain a more complete...

... that everyone can access data and drafts of manuscripts and grant applications in real-time so they can...figure out are timelines for experiments and manuscripts, who is responsible for which parts of the project...

...called JEDI-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21... 1: Summary of the approach and outcomes of the screen for improved genetically-encoded voltage indicators...

...Lentiviral vector design. B) Strategy for leak-free transcriptional control. C) Iterative transduction workflow...of biosensors could be used in high throughput screening for agrochemical compounds that have the potential...

...These sensors generally follow the same pattern described above - a sensing domain induces changes in a ... such as protein or DNA binding, enzyme or transcriptional activation, conformational changes, translation...

...better. More transcription factor binding sites in a promoter lead to higher transcriptional activation....

... and variable spacer sequences. The array is transcribed and processed into crRNAs, each including one...from Addgene!   RNA crRNA: “CRISPR RNAs” are transcribed from the CRISPR array and include a spacer and...

...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the entire series...

...find the vector that fits your experiments, I've described below some of the most frequently requested vectors... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of...

...generally refer to them as the “interested public.” Subscribers to Scientific America would be considered the...scientists in (roughly) the same field, often through manuscripts, seminars, or posters. It is important to note...

...ready-to-use viral preps   Improved cell-free transcription/translation system to examine biosynthetic pathways...pathways  By Gabrielle Clouse Recently, cell-free transcription/translation systems (also referred to as TX-...

...DNA repair (with bonus gRNA design resources.) Subscribe to CRISPR updates on our blog to keep current ...Chris Richardson and members of Jacob Corn’s lab describe how a better understanding of Cas9 biophysics ...