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  1. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...phrase gram negative or positive being used to describe a species of bacteria, but what does it actually...What is a plasmid? Plasmids 101: Blue-white screeningPlasmids 101: Common lab E. Coli strains  ...

  2. Hot Plasmids - January 2023

    Type
    Blog Post
    ... knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm presents an attractive...! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol...

  3. Four Factors that Differentiate the Stem Cell Field

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    Blog Post
    ...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...scientific communities, but usually I only get to scratch the surface of different fields. This meeting has...

  4. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...that lab notebook from 2 years ago. The free subscription to Lab Archives allots for 25 MB of space per...only need to skim the alphabet on the side of the screen to find the sequence for your enzyme of interest...

  5. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact that coiled R-...both lower and higher pH. We initially used this screen to find R body mutants that couldn’t extend at ...

  6. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...mammalian plasmid components, it may be useful to describe the means of introducing genetic material (such...the plasmid in the same way. Selection methods, described below, are typically employed when creating stable...

  8. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants. Several... integration of oligonucleotide libraries of a discrete size at the target site. The use of such libraries...

  9. Viral Vectors 101: Viral Vector Elements

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    Blog Post
    ...Pol, the last component, contains the reverse transcriptase and integrase components to convert the cargo...the primary viral polymerase. E4/E1A activate transcription of other relevant viral genes (including the...

  10. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...single-stranded RNA viruses that use reverse transcriptase to synthesize complementary DNA copies of their... such as a heterologous envelope or a post-transcriptional regulatory element can be used for either type...

  11. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...trillion connections (synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it..., 2022) and substantia nigra (Tervo, 2016). Discrepancies in transduction efficiency can have a major ...

  14. CRISPR 101: Non-Homologous End Joining

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    Blog Post
    ...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...Genome-Wide Control for Large-Scale Functional Screens        ...

  18. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...

  19. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined...

  20. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...
Showing: 341 - 360 of 776 results