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We narrowed to 665 results for: SIM;

Showing: 341 - 360 of 665 results
  1. Viral Vectors 101: Virus Safety

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    Blog Post
    ...researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene...classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate into the host...
  2. Antibodies 101: Choosing the Right Isotype

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    Blog Post
    ... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice... from each other. In a direct approach, that is simple - but what about when you need to use an indirect...
  3. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post
    ...their parents. Here’s how to cross mice, using a simple example with the goal is to generate mice that ...raised in the same environment and will harbor similar epigenetic modifications. Special breeding cases...
  4. Progress Towards a PAM-Free CRISPR

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    Blog Post
    ...cutting most anywhere within the RNA will do. Similarly, if you want to knock out a gene, a frameshift...enzymes   The solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that...
  5. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post
    ...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...et al. suggest that AsCpf1’s off-target rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9...
  6. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post
    ...OpenWorm project. The main goal of OpenWorm is to simulate C. elegans as a computer model. When I first heard...genome. While C. elegans are often thought of as simple organisms , they have many levels of complexity...
  7. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post
    ...transfer plasmid. The good news is there’s a few simple ways to deal with this issue. One way is to grow...cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging PEI transfections...
  8. Plasmids 101: Protein Expression

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    Blog Post
    ...insect cells require demanding culture conditions similar to mammalian expression systems. Bacterial expression...expression. Protein expression in bacteria is quite simple; DNA coding for your protein of interest is inserted...
  9. Common Injection Routes in Mice

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    Blog Post
    ...administered through the tail vein — many researchers will simply refer to these as “tail vein injections.” However...Intranasal instillation Intranasal instillation is very similar to its intratracheal counterpart, as both procedures...
  10. Magnetic Control of Proteins: More than a Dream

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    Blog Post
    ...see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling... tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing...
  11. Viral Vectors 101: Producing Your rAAV

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    Blog Post
    ... helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication. The ...purify your rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of ...
  12. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post
    ...vectors can be used to tag endogenous genes and simultaneously introduce a selection marker for screening ... CRISPR/Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR...
  13. Viral Vectors 101: Voltage Indicators

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    Blog Post
    ... change across the membrane of cardiac cells. Similarly, neuronal signaling is carried out by action potentials...detailed below. Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to...
  14. PhD Applications After COVID

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    Blog Post
    ... tends to go something like this:   However, as simply as it seems laid out above, there are many places...potential programs and universities stressful or simply not feasible. Through the virtual systems created...
  15. Polymerase Chain Reaction Overview and Applications

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    Blog Post
    ...End point PCR, which is commonly referred to as simply PCR, is used for an array of different molecular... or pathogen identification. Scientists can simultaneously detect several different pathogens in one specimen...
Showing: 341 - 360 of 665 results