We narrowed to 665 results for: SIM;

...researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene...classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate into the host...

... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice... from each other. In a direct approach, that is simple - but what about when you need to use an indirect...

... the digital music revolution hit. I predict a similar revolution for antibodies. Recombinant antibody...that may not work well in animals because of similarities to host proteins. After the antibody of interest...

...their parents. Here’s how to cross mice, using a simple example with the goal is to generate mice that ...raised in the same environment and will harbor similar epigenetic modifications. Special breeding cases...

...and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle tissue as it applies to ...

...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability... albino mice ranging from 11% to 100%, a level similar to other approaches.  Nanoblades can also be used...

...cutting most anywhere within the RNA will do. Similarly, if you want to knock out a gene, a frameshift...enzymes   The solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that...

...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...et al. suggest that AsCpf1’s off-target rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9...

...OpenWorm project. The main goal of OpenWorm is to simulate C. elegans as a computer model. When I first heard...genome. While C. elegans are often thought of as simple organisms , they have many levels of complexity...

...transfer plasmid. The good news is there’s a few simple ways to deal with this issue. One way is to grow...cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging PEI transfections...

...insect cells require demanding culture conditions similar to mammalian expression systems. Bacterial expression...expression. Protein expression in bacteria is quite simple; DNA coding for your protein of interest is inserted...

...administered through the tail vein — many researchers will simply refer to these as “tail vein injections.” However...Intranasal instillation Intranasal instillation is very similar to its intratracheal counterpart, as both procedures...

...see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling... tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing...

... helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication. The ...purify your rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of ...

...vectors can be used to tag endogenous genes and simultaneously introduce a selection marker for screening ... CRISPR/Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR...

...Compared to humans and mice, flies have a relatively simple genome. They have four chromosomes: the X (and ... phenotypes, check out the Classical Genetics Simulator website. One interesting thing to know is that...

...after transforming them into your cells, you can simply look for blue colonies to determine which cells...cost yet. However, many fluorescent proteins face similar limitations, and many labs are already using these...

... change across the membrane of cardiac cells. Similarly, neuronal signaling is carried out by action potentials...detailed below. Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to...

... tends to go something like this:   However, as simply as it seems laid out above, there are many places...potential programs and universities stressful or simply not feasible. Through the virtual systems created...

...End point PCR, which is commonly referred to as simply PCR, is used for an array of different molecular... or pathogen identification. Scientists can simultaneously detect several different pathogens in one specimen...