We narrowed to 712 results for: STO

1. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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   ...tagged HA frankenbody with an mCherry, 4xHA tagged histone protein (H2B) in U2OS cells. They then showed that...frankenbody probe could also bind a 1xHA tagged histone protein as well as a mitochondrial protein tagged...

2. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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   ..., 2015). At its very core, peer-review is a touchstone of the scientific method, ensuring that published... and Records: The Royal Society Journal of the History of Science 69.3 (2015): 337-352. PubMed PMID: 26495581...

3. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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   ...endocytosis inhibitor sucrose. Previous work by Stoneham et al. demonstrated that the entry of AAV2 into...157.1 (2012): 228-236. PubMed PMID: 22154564. 6. Stoneham, Charlotte A., Michael Hollinshead, and Amin Hajitou...

4. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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   ...line equipped with photodissociable EB1 could be stopped in areas with blue light - a finding which opens...New tools for developing new FPs - the mCarmine story Article contributed by Caroline LaManna  Listen ...

5. CRISPR Challenges: Standardization and Homology Directed Repair

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   ...Blugene and I represented Addgene at the recent Keystone meeting on Precision Genome Editing with Programmable...publications CRISPOR gRNA design tool Researchers at the Keystone meeting had great things to say about CRISPOR,...

6. In Vivo Biotinylation of Bacterial Fusion Proteins

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   ...after subtracting out non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins...protein folding (tested using real science). Make a stock solution of 2.5 mM biotin (50X) because 5 mM is ...

7. Science Careers: Unruly Interests Feed Many Paths

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   ...network. Which planet will you visit? Where will you stop for refreshment along the way? What ecosystem makes...developmental programs unfold, absorbing the stochasticity of molecular interactions and embedding individual...

8. Plasmids 101: TOPO Cloning

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   ...this sequence. The energy from this breakage is stored as a covalent bond between the cleaved 3′ DNA strand...are planning to transform right away OR you can store the reaction at -20C overnight. 4. Transform TOPO...

9. Plasmid Cloning by PCR

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   ... want to clone from the start codon (ATG) to the stop codon (TGA, in this example). Assuming you are amplifying...taking the final 18 bases of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding...

10. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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    ...large conference room puts butterflies in your stomach, the virtual approach might be less stressful. ...meant that the posters could be viewed publicly and stored in one place if the participants used the conference...

11. Plasmids 101: Gram Negative and Positive Bacteria

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    ...classification system, including its history, biology, and usages in the lab. History In 1884, while studying infected...

12. Improving Transduction Efficiency with Exosome AAVs

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    ...different cellular environments such as retina or glioblastoma). They also found that exo-AAVs were more resistant...brain tumor stromal cells in a mouse model of glioblastoma (Volak et al. 2018). The structure of exosome...

13. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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    ...Julio Ernesto Valdivia Silva, Universidad de Ingeniería y Tecnología (UTEC), Peru Julio Ernesto Valdivia...

14. The Importance of High Titer for AAV Transductions

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    ... mechanism for this function is not yet fully understood, we can imagine that this transport is probably... possible, use low-bind or siliconized tubes to store samples as it is more difficult for viral particles...

15. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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    ...were many excellent applicants but Xin and Fatima stood out for their creativity in the use of fluorescent...shutting off the enzyme. Later exposure to cyan light stopped Dronpa oligomerization and turned the enzymes back...

16. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    ...first post here. Once you’ve identified some fly stocks and other reagents of interest, the next question...regulated by any chosen promoter, and Bloomington’s Stock Center has fly lines expressing Gal4 under a variety...

17. Addgene Depositors Get More Citations

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    ... these plasmids. While clearly an outlier, this story had us wondering: is there a larger trend here? ...also get many more citations This isn’t the whole story, though. It would also be great to know how much...

18. Science Career Options

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    ...are needed in a variety of roles, including: Livestock Breeder Scientific Illustrator Plant Breeder ...hobbies and volunteer experiences, as well as work history can really show that you have some of the abilities...

19. With Our New Viral Service, We're Taking CRISPR Further!

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    ...format. Finally, while we can’t currently produce custom gRNA-containing lentivirus, we are providing lentiviral...packaging plasmids to create your own lentivirus stocks. Our research scientists have provided their standard...

20. Cultivating Community Science at BosLab

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    ...sets. Kellen Andrilenas is a Ph.D. grad student at Boston University studying mammalian immunology, and in... get involved with DIY bio If you’re not in the Boston area but are inspired to get involved, check out...