We narrowed to 729 results for: STO

...the fundamentals. Our mission is to curate a one-stop reference guide for plasmids, so that you can spend...and references, and how to maintain your plasmid stocks. The guide also contains multiple protocols and...

...collapse E. coli re-express arg1 gas vesicles and restore their ultrasound signal. Using acoustic reporter...range of  the ~1011 commensal bacteria per gram of stool for humans. Bourdaeu et al also used arg1 to in ...

...the gene, we can easily annotate it by making a custom feature in Snapgene. This saves us time as we are...restriction enzymes, but you will also be able to create custom annotations and design additional experiments like...

...sharing services, as well as #happydepositors who stopped by to say hello. Needless to say we all got very...the conference and talked to some scientists who stopped by the booth about amazing work already represented...

...network. Which planet will you visit? Where will you stop for refreshment along the way? What ecosystem makes...developmental programs unfold, absorbing the stochasticity of molecular interactions and embedding individual...

...colony and do not subculture directly from glycerol stocks, agar stabs, or liquid cultures. Freshly transformed...plasmid yield that colonies streaked from a glycerol stock. Incubation for 12-16 hours tends to give higher...

...selectively remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is ...

...tagged HA frankenbody with an mCherry, 4xHA tagged histone protein (H2B) in U2OS cells. They then showed that...frankenbody probe could also bind a 1xHA tagged histone protein as well as a mitochondrial protein tagged...

..., 2015). At its very core, peer-review is a touchstone of the scientific method, ensuring that published... and Records: The Royal Society Journal of the History of Science 69.3 (2015): 337-352. PubMed PMID: 26495581...

...interest for which you have several antibodies in stock. Why are there so many options for a single target...samples. If, however, you are performing an immunohistochemistry assay, you may want to rule out monoclonal...

...endocytosis inhibitor sucrose. Previous work by Stoneham et al. demonstrated that the entry of AAV2 into...157.1 (2012): 228-236. PubMed PMID: 22154564. 6. Stoneham, Charlotte A., Michael Hollinshead, and Amin Hajitou...

...line equipped with photodissociable EB1 could be stopped in areas with blue light - a finding which opens...New tools for developing new FPs - the mCarmine story Article contributed by Caroline LaManna  Listen ...

...Blugene and I represented Addgene at the recent Keystone meeting on Precision Genome Editing with Programmable...publications CRISPOR gRNA design tool Researchers at the Keystone meeting had great things to say about CRISPOR,...

...after subtracting out non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins...protein folding (tested using real science). Make a stock solution of 2.5 mM biotin (50X) because 5 mM is ...

...this sequence. The energy from this breakage is stored as a covalent bond between the cleaved 3′ DNA strand...are planning to transform right away OR you can store the reaction at -20C overnight. 4. Transform TOPO...

... want to clone from the start codon (ATG) to the stop codon (TGA, in this example). Assuming you are amplifying...taking the final 18 bases of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding...

...sets. Kellen Andrilenas is a Ph.D. grad student at Boston University studying mammalian immunology, and in... get involved with DIY bio If you’re not in the Boston area but are inspired to get involved, check out...

...were many excellent applicants but Xin and Fatima stood out for their creativity in the use of fluorescent...shutting off the enzyme. Later exposure to cyan light stopped Dronpa oligomerization and turned the enzymes back...

...first post here. Once you’ve identified some fly stocks and other reagents of interest, the next question...regulated by any chosen promoter, and Bloomington’s Stock Center has fly lines expressing Gal4 under a variety...

... these plasmids. While clearly an outlier, this story had us wondering: is there a larger trend here? ...also get many more citations This isn’t the whole story, though. It would also be great to know how much...