Addgene

We narrowed to 410 results for: SUA

Showing: 341 - 360 of 410 results

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Plasmids 101: Multicistronic Vectors

Type

Blog Post

...below. IRES Elements Translation in eukaryotes usually begins at the 5’ cap so that only a single translation...
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Type

Blog Post

... for several different fluorescent proteins. Visualizing fluorescence can be challenging in plants because...
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Type

Blog Post

... the keyboards and displays for control and visualization. Addgene: What do you envision for the future...
MXS Chaining

Type

Blog Post

...these components, one can generate constructs to visualize cell cycle dynamics, titrate inducible transgene...
Viral Vectors 101: The Retroviral Lifecycle

Type

Blog Post

...disease. The gamma-retroviruses have their own unusual sort of virulence as well — namely that they can...
Getting to Know Expansion Microscopy

Type

Blog Post

...Damstra HG, Mohar B, Eddison M, et al (2022) Visualizing cellular and tissue ultrastructure using Ten-...
CRISPR 101: Making a Knock-In Cell Line

Type

Blog Post

...relies on repair from a template molecule, which is usually an endogenously available sister chromatid, though...
AAVs in Retinal Gene Therapy

Type

Blog Post

...characterized and found to suffer from early and severe visual impairment that faithfully recapitulated the human...
SciComm: Taking Science to Elementary and Middle Schools

Type

Blog Post

...they do with their discretionary money. They also usually have a good event-planning base and will know how...
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Type

Blog Post

...we began any sophisticated analyses, we first visualized our data in various ways. Smoothing our plasmid...
Droplet Digital PCR for AAV Quantitation

Type

Blog Post

...range of the assay for a wide range of titers. We usually dilute our samples 1:6 million to 1:25 million....
Simplify Cloning with in vivo Assembly

Type

Blog Post

...sequences are not easy to amplify by PCR, so we usually add DMSO (3%) and Betaine (1 M) to the PCR mix....
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

...require long-term expression of Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may...
Lentiviral Vector Uses and Overview

Type

Blog Post

...transcripts). A different viral envelope protein (Env), usually from VSV-G due to wide infectivity, is often used...
Cpf1: A New Tool for CRISPR Genome Editing

Type

Blog Post

...non-homologous endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due to its proximity to the...
Your Lentiviral Plasmid FAQs Answered

Type

Blog Post

...potential generation of replication competent virus [usually HIV-1]; and 2) the potential for oncogenesis through...
Plasmids 101: Stringent Regulation of Replication

Type

Blog Post

...imposed on the host with a beneficial function (usually antibiotic resistance). In the lab, low copy plasmids...
How to Write a Scientific Review Article

Type

Blog Post

...Finally, you can sound credible and cool when you casually mention “Haery et al., showed that MYC expression...
Transferable Skills Guide: External Collaborations

Type

Blog Post

...are aware of a problem or a need for something, usually someone else has realized this too. Begin with ...
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

Type

Blog Post

...analysis tools have been developed to help parse and visualize expression data in useful ways. Zebrafish mutants...

Showing: 341 - 360 of 410 results

Results per page:

Plasmids 101: Multicistronic Vectors

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

MXS Chaining

Viral Vectors 101: The Retroviral Lifecycle

Getting to Know Expansion Microscopy

CRISPR 101: Making a Knock-In Cell Line

AAVs in Retinal Gene Therapy

SciComm: Taking Science to Elementary and Middle Schools

The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Droplet Digital PCR for AAV Quantitation

Simplify Cloning with in vivo Assembly

CRISPR 101: Ribonucleoprotein (RNP) Delivery

Lentiviral Vector Uses and Overview

Cpf1: A New Tool for CRISPR Genome Editing

Your Lentiviral Plasmid FAQs Answered

Plasmids 101: Stringent Regulation of Replication

How to Write a Scientific Review Article

Transferable Skills Guide: External Collaborations

Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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