Of Myc and Men
Type
Blog Post
...endoplasmic reticulum localization, Munro and Pelham casually introduced the Myc-tag (Munro, et al. 1986; Munro...
We narrowed to 424 results for: SUA
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Genome-wide Screening Using CRISPR
TypeBlog Post...error-prone non-homologous end joining (NHEJ), usually results in a loss-of-function mutation within the... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...tissues transparent are enabling microscopes to visualize the cellular architecture of whole tissues in ... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...below. IRES Elements Translation in eukaryotes usually begins at the 5’ cap so that only a single translation... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post... for several different fluorescent proteins. Visualizing fluorescence can be challenging in plants because... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post... the keyboards and displays for control and visualization. Addgene: What do you envision for the future... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...potentially impacting downstream experiments. The usual suspects, such as proteins, phenol, or guanidine... -
Lentiviral Vector Uses and Overview
TypeBlog Post...transcripts). A different viral envelope protein (Env), usually from VSV-G due to wide infectivity, is often used... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...we began any sophisticated analyses, we first visualized our data in various ways. Smoothing our plasmid... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post...range of the assay for a wide range of titers. We usually dilute our samples 1:6 million to 1:25 million.... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...sequences are not easy to amplify by PCR, so we usually add DMSO (3%) and Betaine (1 M) to the PCR mix.... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...require long-term expression of Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may... -
AAVs in Retinal Gene Therapy
TypeBlog Post...characterized and found to suffer from early and severe visual impairment that faithfully recapitulated the human... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...they do with their discretionary money. They also usually have a good event-planning base and will know how... -
MXS Chaining
TypeBlog Post...these components, one can generate constructs to visualize cell cycle dynamics, titrate inducible transgene... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...disease. The gamma-retroviruses have their own unusual sort of virulence as well — namely that they can... -
Getting to Know Expansion Microscopy
TypeBlog Post...Damstra HG, Mohar B, Eddison M, et al (2022) Visualizing cellular and tissue ultrastructure using Ten-... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...relies on repair from a template molecule, which is usually an endogenously available sister chromatid, though... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...non-homologous endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due to its proximity to the... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post...potential generation of replication competent virus [usually HIV-1]; and 2) the potential for oncogenesis through...