We narrowed to 421 results for: SUA

1. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   Blog Post

   ... for several different fluorescent proteins. Visualizing fluorescence can be challenging in plants because...

2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   Blog Post

   ... the keyboards and displays for control and visualization. Addgene: What do you envision for the future...

3. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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   Blog Post

   ...potentially impacting downstream experiments. The usual suspects, such as proteins, phenol, or guanidine...

4. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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   Blog Post

   ...we began any sophisticated analyses, we first visualized our data in various ways. Smoothing our plasmid...

5. Droplet Digital PCR for AAV Quantitation

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   ...range of the assay for a wide range of titers. We usually dilute our samples 1:6 million to 1:25 million....

6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

   ...require long-term expression of Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may...

7. AAVs in Retinal Gene Therapy

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   Blog Post

   ...characterized and found to suffer from early and severe visual impairment that faithfully recapitulated the human...

8. MXS Chaining

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   Blog Post

   ...these components, one can generate constructs to visualize cell cycle dynamics, titrate inducible transgene...

9. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ...they do with their discretionary money. They also usually have a good event-planning base and will know how...

10. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...sequences are not easy to amplify by PCR, so we usually add DMSO (3%) and Betaine (1 M) to the PCR mix....

11. Viral Vectors 101: The Retroviral Lifecycle

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    ...disease. The gamma-retroviruses have their own unusual sort of virulence as well — namely that they can...

12. Getting to Know Expansion Microscopy

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    Blog Post

    ...Damstra HG, Mohar B, Eddison M, et al (2022) Visualizing cellular and tissue ultrastructure using Ten-...

13. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post

    ...relies on repair from a template molecule, which is usually an endogenously available sister chromatid, though...

14. Lentiviral Vector Uses and Overview

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    Blog Post

    ...transcripts). A different viral envelope protein (Env), usually from VSV-G due to wide infectivity, is often used...

15. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ...non-homologous endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due to its proximity to the...

16. Your Lentiviral Plasmid FAQs Answered

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    Blog Post

    ...potential generation of replication competent virus [usually HIV-1]; and 2) the potential for oncogenesis through...

17. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    Blog Post

    ... only around 400 amino acids in size. To give a visual analogy: if Cas9 or Cas12a is a soccer ball, TnpB...

18. QC Sequencing Technologies at Addgene

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    Blog Post

    ...raw data.  Addgene’s de novo assembly pipeline usually generates the correct plasmid sequence. However...

19. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    Blog Post

    ...basics of the process. Also refer to Figure 1 for a visual overview! Figure 1: General overview of ...

20. Plasmids 101: Stringent Regulation of Replication

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    Blog Post

    ...imposed on the host with a beneficial function (usually antibiotic resistance).  In the lab, low copy plasmids...