We narrowed to 447 results for: SUP

1. Addgene's Tips for Plasmid Quality Control

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   Blog Post

   ...growth instructions (temperature, strain, media supplements), suggested sequencing primers, or mammalian ...

2. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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   ...to all results in the relation to how they are supporting the conclusions. Look at the figures and write...

3. Working with Nuclear Receptors

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   ...transcription factors found in metazoans. The superfamily is comprised of 48 human and 49 murine members...

4. Early Career Researcher Toolbox: Finding Relevant Papers

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   ...about publications in the fields of research they support. Monitoring these types of accounts can help you...

5. Engaging with science and society at pgEd

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   ... experiment, the case of Henrietta Lacks, the Havasupai incident, and the sterilization of women in marginalized...

6. Overcoming the Challenges of Lentiviral Production

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   ...cells are incubated and after several days the supernatant is collected, filtered, and titered. A major ...

7. Popular Retroviral Vectors and Their Uses in Scientific Research

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   ...Perturbation Platform, to produce their shRNA library to support functional investigations of the mammalian genome...

8. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ...different Arg-mutant enzymes could be rescued by supplementation with guanidine hydrochloride (GdnHCl). Rescue...

9. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   ...Proceedings of the National Academy of Sciences 100.suppl 1 (2003): 11861-11865. PubMed PMID: 12917490. PubMed...

10. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    ...ura3 mutants and cannot grow without uracil supplementation (6). Expression of MET/Met genes from the pYES2.1...

11. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...basic research and other ongoing clinical trials support the utility of AAV as a broadly applicable gene...

12. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...required. On the other hand, lentiviral vectors are superior packaging vectors for larger genes - they can ...

13. Open Resources and Plasmid Tools For Studying C. elegans

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    ...biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed PMID: 17986455. PubMed ...

14. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ...time. Antibodies made from animals are limited in supply and producing antibodies from plasmids is much ...

15. Antibodies 101: Choosing the Right Isotype

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    ...available secondary antibodies and found that across suppliers there was a detection bias of IgG2a>IgG2b>IgG1...

16. Plasmids 101: Modular Cloning

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    ...phenotype, translational output, etc., which can support a larger cellular engineering goal, several of ...

17. Gaining Leadership Skills Volunteering at a Professional Organization

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    ...invited to spend a weekend together, funded by supporters such as the Burroughs Wellcome Trust. These “...

18. Experimenting in an Open Source lab: from CRISPR to Cats

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    ...contributes each month - with only occasional supplementation with grant funds, money is always tight. Of...

19. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...They are isolated mostly from abnormal and/or supernumerary preimplantation embryos that are produced during...

20. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post

    ...PEG precipitation of the HEK293 cell culture supernatant because it’s time consuming to prepare the 40%...