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Showing: 341 - 360 of 617 results

  3. Of Myc and Men

    Type
    Blog Post
    ... the same target. In the 1980s, Munro and Pelham set out to address this issue by developing generic strategies...

  4. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ... notice for the first meeting so that people can set aside time in their schedules and read the paper ...). This notice can be shortened as the meeting settles in and people get the time recurrently carved out...

  8. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...allow for two sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus...protocol for MMEJ-mediated knock-in of a GFP-Puro cassette into a given locus, just upstream of a stop codon...microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks are necessary for...for knock-in: one on either side of the GFP-Puro cassette and one in between the 5’ and 3’ microhomologies...of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and PITCh-gRNA...creates two DSBs in the PITCh plasmid to release the cassette, and an insertion-locus-specific gRNA cleaves ...and 3’ microhomologies are added to the GFP-Puro cassette via PCR, and this construct is inserted into the...

  10. Weighing Reagents Protocol

    Type
    Protocol
    ...measurement. Hit the tare button (or zero button) to set the balance to zero. Pro-Tip If you’re weighing a...

  11. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...

  13. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...antigen or an antibody depending on how the assay is set up. Then they block the capture reagent to prevent...binding to a primary antibody.  While the precise setup of the method can vary, typically users first coat...

  14. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...facilitates the use of prime editing in pooled settings with hundreds to thousands of pegRNAs. Chromatin-based...primer binding site, and many more. Once we had our datasets with all the different features and editing efficiencies...PRIDICT2.0 (Mathis et al., 2024). The additional datasets used in the training of PRIDICT2.0 make this model...

  15. Hot Plasmids - August 2020

    Type
    Blog Post
    ...be useful for your studies.  Find the plasmids to set up your screening systems at Addgene! Schematic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    ...working on one process at a time, and testing a small set of data each time a new process was added, we progressively...easily shared with others without any complicated setup. We created, tested, and debugged each of our pipeline...

  18. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...mice CRISPRa has largely been limited to in vitro settings due to the large size of Cas9 activators, which...packaging limits (i.e., how many sgRNA expression cassettes will fit). Cre-dependent expression of the SPH...

  19. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...the day of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors you want...research? Do you have innovative tools? A novel dataset? Unique expertise? A novel hypothesis? Part of ...
Showing: 341 - 360 of 617 results