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Showing: 341 - 360 of 612 results

  3. Of Myc and Men

    Type
    Blog Post
    ... the same target. In the 1980s, Munro and Pelham set out to address this issue by developing generic strategies...

  6. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...allow for two sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus...protocol for MMEJ-mediated knock-in of a GFP-Puro cassette into a given locus, just upstream of a stop codon...microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks are necessary for...for knock-in: one on either side of the GFP-Puro cassette and one in between the 5’ and 3’ microhomologies...of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and PITCh-gRNA...creates two DSBs in the PITCh plasmid to release the cassette, and an insertion-locus-specific gRNA cleaves ...and 3’ microhomologies are added to the GFP-Puro cassette via PCR, and this construct is inserted into the...

  8. Weighing Reagents Protocol

    Type
    Protocol
    ...measurement. Hit the tare button (or zero button) to set the balance to zero. Pro-Tip If you’re weighing a...

  9. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...mice CRISPRa has largely been limited to in vitro settings due to the large size of Cas9 activators, which...packaging limits (i.e., how many sgRNA expression cassettes will fit). Cre-dependent expression of the SPH...

  10. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...the day of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors you want...research? Do you have innovative tools? A novel dataset? Unique expertise? A novel hypothesis? Part of ...

  11. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...have become a powerful tool to address a diverse set of biological inquiries. References Ogura, Teru...death. The team used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed...for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct repeats which can be ... selects for those cells that have removed the cassette through a second recombination event between the...

  14. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... used successfully in preclinical and clinical settings for ocular conditions. However, AdV was abandoned...responsible for an autosomal recessive, severe, childhood-onset form of blindness name Leber’s Congenital Amaurosis... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...

  15. Hot Plasmids - August 2020

    Type
    Blog Post
    ...be useful for your studies.  Find the plasmids to set up your screening systems at Addgene! Schematic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    ...working on one process at a time, and testing a small set of data each time a new process was added, we progressively...easily shared with others without any complicated setup. We created, tested, and debugged each of our pipeline...

  18. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ... the use of a given timer in many experimental settings. Monomeric fluorescent protein timers Since the... simplifies cloning and makes your experimental setup less cumbersome. 3. Portability across multiple ...

  19. MXS Chaining

    Type
    Blog Post
    ...sequences of the genetic elements may differ (e.g., a set of various mammalian promoters), the same cloning...sequences. Intermediate chaining products (called “cassettes”) can be readily reused in the assembly of any...any later construct (i.e.,functional cassettes are recyclable) Can only work if the restriction sites used...

  20. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...
Showing: 341 - 360 of 612 results