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We narrowed to 616 results for: Set;

Showing: 341 - 360 of 616 results
  1. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ... notice for the first meeting so that people can set aside time in their schedules and read the paper ...). This notice can be shortened as the meeting settles in and people get the time recurrently carved out...
  2. Of Myc and Men

    Type
    Blog Post
    ... the same target. In the 1980s, Munro and Pelham set out to address this issue by developing generic strategies...
  3. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...allow for two sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus...protocol for MMEJ-mediated knock-in of a GFP-Puro cassette into a given locus, just upstream of a stop codon...microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks are necessary for...for knock-in: one on either side of the GFP-Puro cassette and one in between the 5’ and 3’ microhomologies...of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and PITCh-gRNA...creates two DSBs in the PITCh plasmid to release the cassette, and an insertion-locus-specific gRNA cleaves ...and 3’ microhomologies are added to the GFP-Puro cassette via PCR, and this construct is inserted into the...
  4. Weighing Reagents Protocol

    Type
    Protocol
    ...measurement. Hit the tare button (or zero button) to set the balance to zero. Pro-Tip If you’re weighing a...
  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ... the use of a given timer in many experimental settings. Monomeric fluorescent protein timers Since the... simplifies cloning and makes your experimental setup less cumbersome. 3. Portability across multiple ...
  6. MXS Chaining

    Type
    Blog Post
    ...sequences of the genetic elements may differ (e.g., a set of various mammalian promoters), the same cloning...sequences. Intermediate chaining products (called “cassettes”) can be readily reused in the assembly of any...any later construct (i.e.,functional cassettes are recyclable) Can only work if the restriction sites used...
  7. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... used successfully in preclinical and clinical settings for ocular conditions. However, AdV was abandoned...responsible for an autosomal recessive, severe, childhood-onset form of blindness name Leber’s Congenital Amaurosis... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...
  8. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...
  9. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...antigen or an antibody depending on how the assay is set up. Then they block the capture reagent to prevent...binding to a primary antibody.  While the precise setup of the method can vary, typically users first coat...
  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...facilitates the use of prime editing in pooled settings with hundreds to thousands of pegRNAs. Chromatin-based...primer binding site, and many more. Once we had our datasets with all the different features and editing efficiencies...PRIDICT2.0 (Mathis et al., 2024). The additional datasets used in the training of PRIDICT2.0 make this model...
Showing: 341 - 360 of 616 results