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We narrowed to 605 results for: She

Showing: 341 - 360 of 605 results

  2. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...structure and could also cause your target to be washed away. Furthermore, these chemicals can also alter...: Always always always look at your antibody datasheet to see if it has been tested in IF and if a protocol...

  5. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...wired into circuits through synaptic connections (Shepherd, 2004). Understanding the structure and function...25915120. PubMed Central PMCID: PMC4573404. 2. Shepherd, G.M. (2004). In "The synaptic organization of...

  6. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...implementation, and now must focus on managing the established partnership. If you’re willing to do similar ...meetup or recruit volunteers for your local animal shelter. There are many opportunities so just pick the ...

  8. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...combined all the domains into a single protein, shedding the complexity of its ancestors. They classified...CRISPR 101: RNA Editing With Cas13  CRISPR Cheat Sheet Resources at Addgene.org CRISPR Guide...

  9. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...strategy Figure 4 shows a gating strategy from a published experiment (Barlow-Anacker et al., 2017). This...Neonatal immune response to rhinovirus A16 has diminished dendritic cell function and increased B cell ...

  10. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...For more details, check out this review article published in Reviews in Medical Virology (Li et a., 2018...doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL, Jolly DJ, Dubensky ...

  11. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...and the replication control machinery (Need a refresher on the Origin of Replication? See our Plasmids....2015.03.007 Pubmed PMID: 25889267 Ebersbach G, Sherratt DJ, Gerdes K (2005) Partition-associated incompatibility...

  12. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...Small’ data produced by the research group in spreadsheets, Word docs, and the like is likely stored in... documents, but also to the associated data (spreadsheets, images, sequencing data, etc.) used and/or ...

  13. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...continual production of MazE, the toxin becomes active. Shen Yang’s lab made use of mazF as a counterselectable...PMID: 12718874. Yang, Junjie, Weihong Jiang, and Sheng Yang. "mazF as a counter-selectable marker for unmarked...

  14. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...conditions different from those specified on its data sheet?” or “Do you have additional data regarding the ...that matter. Therefore, if there is a bit of a Sherlock Holmes in you – a technical support role is the...

  15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ... (Wu et al., 2004). Over time, the expression diminishes as the episomes are lost through cycles of cell...K., Schmidt, M., von Kalle, C., Gaspar, H. B., Thrasher, A. J. (2008). Insertional mutagenesis combined...

  16. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...have a good mixture of useful tasks that can be accomplished quickly and things that will take longer. Completing...Throughout the day, I cross items off as they are accomplished or I add to the list as the need arises.Why ...

  17. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the... including the development of crops with longer shelf life and the development of disease-resistant crops...

  18. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...offended. I felt terrible when the host told me and wished I had been more careful. 3. Tell a story We are...thinking “What happens next?”  Describe how you pushed to a solution or overcame obstacles. Take time ...

  19. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...enough T-cells could be reached to ablate a well-established viral reservoir. A second challenge for CRISPR...developing resistance. Shortly after Kaminski et al. published their results, Wang et al. showed that HIV-1 could...

  20. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ... treat disease postnatally. Now, three papers published concurrently in Science have shown CRISPR can ...McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal genome editing...
Showing: 341 - 360 of 605 results