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We narrowed to 609 results for: She

Showing: 341 - 360 of 609 results

  1. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits...

  3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...structure and could also cause your target to be washed away. Furthermore, these chemicals can also alter...: Always always always look at your antibody datasheet to see if it has been tested in IF and if a protocol...

  6. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...continual production of MazE, the toxin becomes active. Shen Yang’s lab made use of mazF as a counterselectable...PMID: 12718874. Yang, Junjie, Weihong Jiang, and Sheng Yang. "mazF as a counter-selectable marker for unmarked...

  8. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...wired into circuits through synaptic connections (Shepherd, 2004). Understanding the structure and function...25915120. PubMed Central PMCID: PMC4573404. 2. Shepherd, G.M. (2004). In "The synaptic organization of...

  9. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...implementation, and now must focus on managing the established partnership. If you’re willing to do similar ...meetup or recruit volunteers for your local animal shelter. There are many opportunities so just pick the ...

  11. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...combined all the domains into a single protein, shedding the complexity of its ancestors. They classified...CRISPR 101: RNA Editing With Cas13  CRISPR Cheat Sheet Resources at Addgene.org CRISPR Guide...

  12. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...strategy Figure 4 shows a gating strategy from a published experiment (Barlow-Anacker et al., 2017). This...Neonatal immune response to rhinovirus A16 has diminished dendritic cell function and increased B cell ...

  13. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...conditions different from those specified on its data sheet?” or “Do you have additional data regarding the ...that matter. Therefore, if there is a bit of a Sherlock Holmes in you – a technical support role is the...

  14. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...For more details, check out this review article published in Reviews in Medical Virology (Li et a., 2018...doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL, Jolly DJ, Dubensky ...

  15. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...and the replication control machinery (Need a refresher on the Origin of Replication? See our Plasmids....2015.03.007 Pubmed PMID: 25889267 Ebersbach G, Sherratt DJ, Gerdes K (2005) Partition-associated incompatibility...

  16. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...Small’ data produced by the research group in spreadsheets, Word docs, and the like is likely stored in... documents, but also to the associated data (spreadsheets, images, sequencing data, etc.) used and/or ...

  17. Pipetting Protocol

    Type
    Protocol
    ...pipette, and the tip ejector ring at the bottom which pushes the pipette tip off of the pipette. This side view...rate into the pipette tip. Once the liquid has finished drawing into the pipette tip, lift the pipette...

  18. Transfection for Recombinant Antibodies

    Type
    Protocol
    ... system, 500 mL, VWR 430770 Trypan Blue, Thermo Fisher T10282 Valproic acid sodium salt, Sigma Aldrich... Grade, VWR 75800-188 10% Poloxamer 188, Thermo Fisher 24040032 Glutagro, Corning 25-015-CI BalanCD HEK293...

  19. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ... (Wu et al., 2004). Over time, the expression diminishes as the episomes are lost through cycles of cell...K., Schmidt, M., von Kalle, C., Gaspar, H. B., Thrasher, A. J. (2008). Insertional mutagenesis combined...

  20. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...have a good mixture of useful tasks that can be accomplished quickly and things that will take longer. Completing...Throughout the day, I cross items off as they are accomplished or I add to the list as the need arises.Why ...
Showing: 341 - 360 of 609 results