...continual production of MazE, the toxin becomes active. Shen Yang’s lab made use of mazF as a counterselectable...PMID: 12718874. Yang, Junjie, Weihong Jiang, and Sheng Yang. "mazF as a counter-selectable marker for unmarked...

...28474669. Pubmed Central PMCID: PMC5424143. 2. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling...Cas9 Review the basics of CRISPR with this cheat sheet Read our Quick Guide to All Things Lentivirus Resources...

...conditions different from those specified on its data sheet?” or “Do you have additional data regarding the ...that matter. Therefore, if there is a bit of a Sherlock Holmes in you – a technical support role is the...

...wired into circuits through synaptic connections (Shepherd, 2004). Understanding the structure and function...25915120. PubMed Central PMCID: PMC4573404. 2. Shepherd, G.M. (2004). In "The synaptic organization of...

...implementation, and now must focus on managing the established partnership. If you’re willing to do similar ...meetup or recruit volunteers for your local animal shelter. There are many opportunities so just pick the ...

...strategy Figure 4 shows a gating strategy from a published experiment (Barlow-Anacker et al., 2017). This...Neonatal immune response to rhinovirus A16 has diminished dendritic cell function and increased B cell ...

...Andreas Rett, who tried to raise awareness and published his observations in a German medical journal. ...in mice is possible (Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the...

...combined all the domains into a single protein, shedding the complexity of its ancestors. They classified...CRISPR 101: RNA Editing With Cas13  CRISPR Cheat Sheet Resources at Addgene.org CRISPR Guide...

...For more details, check out this review article published in Reviews in Medical Virology (Li et a., 2018...doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL, Jolly DJ, Dubensky ...

...and the replication control machinery (Need a refresher on the Origin of Replication? See our Plasmids....2015.03.007 Pubmed PMID: 25889267 Ebersbach G, Sherratt DJ, Gerdes K (2005) Partition-associated incompatibility...

...infection and selection G. Safety H. References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence...Do not use a 0.2 μm filter, as this is likely to shear the envelope of your virus. o. Virus may be stored...Biosafety Guide . Back to Top H. References H.1. Published Articles Functional siRNAs and miRNAs exhibit ...

...exposure. Read the reagent’s material safety data sheet (MSDS) to determine if this is best. Check that ...biohazardous. Check your lab’s material safety data sheets (MSDS) to understand how to properly dispose of...

... (Wu et al., 2004). Over time, the expression diminishes as the episomes are lost through cycles of cell...K., Schmidt, M., von Kalle, C., Gaspar, H. B., Thrasher, A. J. (2008). Insertional mutagenesis combined...

...offended. I felt terrible when the host told me and wished I had been more careful. 3. Tell a story We are...thinking “What happens next?”  Describe how you pushed to a solution or overcame obstacles. Take time ...

...enough T-cells could be reached to ablate a well-established viral reservoir. A second challenge for CRISPR...developing resistance. Shortly after Kaminski et al. published their results, Wang et al. showed that HIV-1 could...

...have a good mixture of useful tasks that can be accomplished quickly and things that will take longer. Completing...Throughout the day, I cross items off as they are accomplished or I add to the list as the need arises.Why ...

...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the... including the development of crops with longer shelf life and the development of disease-resistant crops...

... treat disease postnatally. Now, three papers published concurrently in Science have shown CRISPR can ...McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal genome editing...

...enhanced random integration efficiencies that are accomplished with transfections and are therefore virus-free...enhance gene editing? Gene editing can now be accomplished in a more cost-effective and efficient manner...

...read a paper and saw an interesting construct you wished to use, you could either make it yourself or you...Intracellular Calcium Sensor Yang, J. et al. (7) wished to trigger intracellular Ca++ release with an optogenetic...