We narrowed to 738 results for: Sis

...number. First you generate four unique kanamycin-resistant plasmids, each containing a different gRNA target... all but one of your gRNAs into spectinomycin-resistant plasmids pX330S-2 to pX330S-(last gRNA number).... Yin, Linlin, et al. “Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs...

...engages the prime editor protein. The prime editor consists of a Cas9 nickase fused to a reverse transcriptase... domain for optimization. They developed phage-assisted evolution strategies to evolve more effective ... J., Osborn, M. J., & Liu, D. R. (2023). Phage-assisted evolution and protein engineering yield compact...

...creation of improved fluorescent proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent...guest, Joachim Goεdhart! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

...organisms belong to a group called dicots — think Arabidopsis thaliana. However, many of the world’s food crops...induced by one of several ligands. Plants showed persistent coloration over a month after treatment. Several...

...career landscape and there is insufficient (or no) emphasis on developing transferable skills to enable pursuit...recent discussions of this apparently worsening crisis recently appeared in eLife: "A Fair Deal for PhD...

...you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to bacteria, ...culture without antibiotics to give the antibiotic resistance gene time to be expressed.  However, you can ...

...Saftic) to educational gifs created by the Amoeba Sisters. Here’s one example of how they “bridge the jargon...jargon gap using gifs”: https://amoebasisters.tumblr.com/post/139944465237/lactose-around-we-can-make-...

...lectures targeting three broad concepts: the genetic basis of disease, cancer, and viral infections. An interactive...science, but they didn’t seem to mind! Attendees consistently mentioned how pleased they were to learn about...

...perturbations that may play significant roles in cancer pathogenesis. Their work culminated in the first functional...lentiviral constructs using the High-Throughput Mutagenesis and Molecular Barcoding (HiTMMoB) technique (...

...genes as drivers of peritoneal mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids...peritoneal mesothelioma cells lines and assess their tumorigenesis. These experiments will serve to validate these...

...they also went in search of genes involved in resistance to the cancer drug vemurafenib. Enriched libraries... screen for genes involved in bacterial toxin resistance, identifying 4 previously unknown genes.  “With...

...  Joining the Addgene crew!  This pattern was consistent: everyone I spoke with at Addgene, no matter ...presentation.  My first time in the lab! I got to assist production on a protocol video. Don't worry - no...

...Optogenetic Tools Like pDawn  Making cassava plants resistant to cassava mosaic virus Dr. Fiona Robertson, University... cry genes from the soil bacterium Bacillus thuringiensis and analysing the insecticidal potential of ...

...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...

... evolution took over (with a little mutagenic assistance). Quicker CAST activity allowed certain mutated...evoCAST to the Liu lab’s own PASSIGE (Prime-Editing-Assisted Site-Specific Integrase Gene Editing), which combines...

...microtubules. Tanenbaum et al. then tested their hypothesis that the lower expression levels of SunTag constructs...suitability in your experiments on a protein-by-protein basis. However, since many of Addgene’s SunTag plasmids...

...detection of the tagged protein. These peptides consist of as little as 8 or as many as several hundred...10.1007/s00253-002-1158-6 Baker, M., Reproducibility crisis: Blame it on the antibodies. Nature (2015) 521,...

...Instead, it targets a specific audience - giving consistency, purpose, structure, and intent to the messaging...your audience, and their expectations of you, consistent and clear.   And now that we’re all on the same...

...We believe that this simplified sequencing and analysis approach can be easily incorporated into standard... A Rapid and Robust Assay for Batch-to-Batch Consistency Evaluation of AAV Preparations. Molecular Therapy...

..., 2020. Now that we know all about antibiotic resistance genes, let’s consider another basic element of...for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12...