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We narrowed to 731 results for: Sis;

Showing: 341 - 360 of 731 results

  1. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...as a control. DNA was analyzed by PAGE. The basis of the Surveyor assay is that Surveyor nuclease ...edit. Using Surveyor nuclease followed by gel electrophoresis, these mismatches are indicated by the presence...Figure 4). 5. In theory you could use densitometric analysis to quantitate the intensity of the bands on your...

  2. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide RNAs (sgRNAs), are time-consuming...reagent preparation in the lab. Figure 3: csRNP-assisted epitope tagging is scalable to 96-well format...., Gogolok S et al. 2017. Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic...

  3. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...undesirable side products generated during chemical synthesis of these molecules. To make large amounts of pure...applications, but there are more! Engineering disease resistance through gene stacking, increasing production ...Generation of 8× Nicotiana Benthamiana and 12× Arabidopsis Mutants.” The Plant Journal, vol. 106, no. 1,...

  4. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...depends on it. Deanna McLafferty is an editorial assistant and activist. Washington, DC, USA 38.9072°N 77.0369...scientists – including global warming skeptics – insisted that more data and research funding are critical...trying turn a few exoplanets into a successful PhD thesis. We use the same atmospheric physics to understand...

  5. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...number. First you generate four unique kanamycin-resistant plasmids, each containing a different gRNA target... all but one of your gRNAs into spectinomycin-resistant plasmids pX330S-2 to pX330S-(last gRNA number).... Yin, Linlin, et al. “Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs...

  6. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...engages the prime editor protein. The prime editor consists of a Cas9 nickase fused to a reverse transcriptase... domain for optimization. They developed phage-assisted evolution strategies to evolve more effective ... J., Osborn, M. J., & Liu, D. R. (2023). Phage-assisted evolution and protein engineering yield compact...

  8. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...career landscape and there is insufficient (or no) emphasis on developing transferable skills to enable pursuit...recent discussions of this apparently worsening crisis recently appeared in eLife: "A Fair Deal for PhD...

  9. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...Saftic) to educational gifs created by the Amoeba Sisters. Here’s one example of how they “bridge the jargon...jargon gap using gifs”: https://amoebasisters.tumblr.com/post/139944465237/lactose-around-we-can-make-...

  10. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...lectures targeting three broad concepts: the genetic basis of disease, cancer, and viral infections. An interactive...science, but they didn’t seem to mind! Attendees consistently mentioned how pleased they were to learn about...

  11. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to bacteria, ...culture without antibiotics to give the antibiotic resistance gene time to be expressed.  However, you can ...

  13. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...genes as drivers of peritoneal mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids...peritoneal mesothelioma cells lines and assess their tumorigenesis. These experiments will serve to validate these...

  16. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...microtubules. Tanenbaum et al. then tested their hypothesis that the lower expression levels of SunTag constructs...suitability in your experiments on a protein-by-protein basis. However, since many of Addgene’s SunTag plasmids...

  17. An Addgene Summer Internship

    Type
    Blog Post
    ...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...

  19. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ... evolution took over (with a little mutagenic assistance). Quicker CAST activity allowed certain mutated...evoCAST to the Liu lab’s own PASSIGE (Prime-Editing-Assisted Site-Specific Integrase Gene Editing), which combines...

  20. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ..., 2020. Now that we know all about antibiotic resistance genes, let’s consider another basic element of...for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12...
Showing: 341 - 360 of 731 results