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We narrowed to 797 results for: Tec

Showing: 341 - 360 of 797 results

  1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...their expression to individual cells. With this technique, a Neuroscientist can see the full dendritic arbor... able to distribute materials containing FLEx technology. Read the genOway press release for more information...Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62. PubMed PMID: 9661200.  3. Cardin...at the Cellular Level in the Mouse.” Nature Biotechnology 21(5): 562–65. PubMed PMID: 12665802. 9. Villa...

  2. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... induced gene silencing (VIGS) is a transient technique that uses a virus and the plant's post-transcriptional...surface) of the leaf. This is a simple and easy technique to implement. Success might vary between experimenters...Boston, MA. He obtained his BSc and MSc in Plant Biotechnology from Leibniz Universität Hannover (Germany),...PMID: 16739580. Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation...

  3. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...Therapy Consortium. Leveraging their combined technical expertise to develop a viable therapeutic, the... and Feng Zhang, PhD, is pursuing CRISPR/Cas9 technology for precise correction of Rett syndrome mutations...three neuronal subtypes of the hippocampus. This technology has formed the basis of the Rett syndrome program...co-founded by Gail Mandel. There are numerous technical challenges to overcome for both of these approaches...

  4. Hot Plasmids - August 2020

    Type
    Blog Post
    ... Mateo Sanchez and Alice Ting have used one biotechnology tool to optimize another. The authors have created...some notable improvements. Directed evolution techniques mimic natural selection of sequences that evolve... proteins at small and large scale.  This new technique involves selection in the cytosol (instead of ...purification required! The team used other molecular techniques to introduce time modulation of the TEV cleavage...

  5. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...characterize, and develop new CRISPR systems and technologies, the AbuGoot lab often tests samples of new ...positions defined by the target:spacer duplex without detectable non-specific activity. Its major advantage is...same comparisons, the AbuGoot lab reported no detectable effect on cell viability – meaning there was ... genes. In comparison with other large insert techniques like HITI or HDR, which require specific repair...

  6. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...into target genomes and a 10-fold improvement in detection efficiency over the original CROPseq. Find CROPseq-multi...the protein La (a small RNA-binding exonuclease protection factor) promotes editing (Yan, J et al., 2024...stabilizing the 3’ end of the pegRNA, which is not well protected by Cas9. This is similar to the proposed mechanism... effort to fill a void in available tools for detecting SARS-CoV-2 (Terry et al., 2021). Check out their...

  7. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... the screening strategies outlined below, the technique can be used as a preliminary validation on your...Decomposition (TIDE) (Brinkman et al., NAR). This technique uses the trace file generated in Sanger sequencing...Sequencing Options for CRISPR Genotyping NGS and detecting off target effects  While pricier, NGS can be ...analysis. If NGS is not an option, you can still detect off-target effects with Sanger sequencing, provided...

  8. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...modifications feasible and even easy. This targeted-break technology coupled with HDR is a powerful site-specific ...the genome, resulting in off-target effects. A technique that pushes the size limitations of ssODNs is ...principles driving CRISPRs and genome editing technology. Links: Zhang Lab’s CRISPR FAQs CRISPR forum...asymmetric donor DNA. Richardson CD, et al. Nat Biotech. 34(3); (2016). PubMed. Efficient homology-directed...

  9. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... illuminates the passing cell with a laser and detects the light scattered by that cell at a low angle...laser beam and causes it to scatter (FSC/SSC), the detected photons can be represented through a graph of ... only enter dead cells, while living cells can protect themselves from it. So here, I actually want to...— describing the intensity and duration of the detected signal. Using a practical example of lymphocytes...

  10. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... events and training courses on technologies and laboratory techniques related to antibody discovery. ...) either produced de novo using yeast display technology or produced from hybridoma antibodies developed...synthetic rAbs, developed using yeast display technology, are directed to integrins that bind the Arginine-Glycine-Aspartate...

  11. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...antibodies: the power of in vitro display technologies. Nat Biotechnol 29:245–254 . https://doi.org/10.1038...of Milstein and Köhler who developed hybridoma technology. But what about single chain fragment variables...living cells. The HA frankenbody is an HA tag detection probe. It contains an HA-binding scFv that’s fused...

  12. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...: Čelešnik, Helena, et al. "Biosafety of biotechnologically important microalgae: intrinsic suicide switch..., Xuechen Zhu, and Charles E. Melançon III. "Detection and Quantification of Ribosome Inhibition by Aminoglycoside...fusions reported in the literature include RNA detection, RNA cleavage, and translational repression or...Genetically Encodable System for Sequence‐Specific Detection of RNAs in Two Colors."ChemBioChem 17.10 (2016...

  13. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...with antiviral therapies such that the virus is undetectable in the blood. But the virus doesn’t completely...respond. Latently infected cells evade immune detection because they produce very little or no viral protein...reinfection. Importantly, no off-target effects were detected. Kaminski et al. observed no negative effects ... field, the lessons learned in pursuing one technological application can benefit many others and accelerate...

  14. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ... IMP USA-Europe not only provide the specific technical and logistic support to increase the chances of...of success, but also nurture a supportive and protective environment where individuals are empowered to...represented a diverse group of individuals with technical and career path needs, and came from the universities...Harvard University, Massachusetts Institute of Technology, Boston University, Johns Hopkins University,...

  15. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Table 1: AAV Serotypes and Tropism Experiment  Detection Method CNS AAV1, AAV2, AAV4, AAV5, AAV8, AAV9...purposes. The University of Pennsylvania and the biotechnology firm Biogen have recently collaborated to develop...is their ability to be used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene... fourth generation of neuroanatomical tracing techniques: exploiting the offspring of genetic engineering...

  16. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...and smaller engineered antibody fragments. One technique we haven’t discussed in depth is antibody directed...large mutant libraries, high-throughput screening techniques such as ELISA and cell surface display are common... in learning more about antibody engineering technologies, the Institute of Protein Innovation (IPI) provides...fragments and the rise of single domains. Nature Biotechnology, 23(9), 1126–1136. https://doi.org/10.1038/nbt1142...

  17. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...scientific team came as an undergrad at Worcester Polytechnic Institute. Though some WPI students chose to ... physical kits are made and shipped; Chiara, the tech transfer expert who understands the somewhat complicated...repository, performing quality control, and providing technical customer service. Team members are highly trained...a biostatistician, and that one golden-handed technician who can do a certain difficult assay with her...

  18. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...HaloTag or SNAP-tag construct can help you better detect dim structures or low levels of expression with...performance and use super-resolution or single-molecule techniques.   Figure 2: Two-color super-resolution...proteins with small molecules in vivo. Nature Biotechnology, 21(1), 1. https://doi.org/10.1038/nbt765  Lavis... V. (2008). HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis. ACS Chemical...

  19. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... label it with finesse using tRNA suppression technology (Fig. 1) (Dumas, Lercher, Spicer, & Davis, 2015... from depositing labs that have mastered this technique so it might be worth a try, especially since the...References 1. Degorce, François, et al. "HTRF: a technology tailored for drug discovery–a review of theoretical...Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic Concept." PloS one 8.5 (2013...

  20. The Twelve Days of CRISPR

    Type
    Blog Post
    ...Addgene gave to me: SHERLOCK and DETECTR, two CRISPR-based methods to detect nucleic acids.  On the third ... CRISPR, Addgene gave to me: five methods for detecting SARS-CoV-2 using CRISPR.  On the sixth day of ...
Showing: 341 - 360 of 797 results