...from countless rounds of optimization. Dozens of variants exist with unique cutting features and evolved...engineering endeavors. For more information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme... detection tool for rare, often hard to detect variants such as single nucleotide polymorphisms. Fast...

... is explained in more detail here. There are a variety of methods that can be used to measure your fluorescent...filtration (Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay ... Of course, you want the ‘latest and greatest’ variants with the best properties for your research. But...

...different fluorescence imaging strategies use widely varying amounts of excitation power (Shaner et al, 2008... An example of a photostability measurement of various cyan fluorescent proteins is depicted in figure...higher excitation power, the photostability of the variants is comparable (lower panel of figure 1). To avoid...

... application. Consider also that there may be variations within an application. Take immunohistochemistry...might find that while the website highlights a variation of your application, a publication successfully...this is the case, you can narrow them down in a variety of ways. First, review the citations. An antibody...

...editing in both bacteria and human cells using various delivery methods, including plasmid, ribonucleoprotein...efficiency of prime editing to correct mutations in a variety of target genes and cell types. Their Nature Biotechnology... Various Addgenies...

...Filip Tomaska and Tim Hanson, are characterizing variants of iGluSnFR3 in vivo and have used it to detect...these cells and the functional contribution to a variety of behaviors related to addiction and pain. Once...biosensors He Tian (goes by “Tian”) is a postdoc at Harvard University where she engineers genetically encoded...

...model, ubi:zebrabow. In this system, cells express various fluorescent proteins controlled by Cre recombination... that recombine independently and results in a variety of colors depending on the combination of fluorescent... Various Addgenies...

...RNA-protein interactions. To address this need, the Khavari lab at Stanford created the RNA-protein interaction...disease. RaPID can also be used to test how RNA variants associated with human disease affect protein binding...Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction detection...

...for oxidizing environments to photoconvertible variants, it seems like GFP is always learning new things...lab researcher Jonathan Tang wanted to express a variety of proteins in single cell types in the mouse, ...proteins with a modular structure, constructing split variants should be relatively straightforward, but it is...

... and some, such as varenicline, are already approved for clinical use. Varenicline has been further modified...promoters, AAV serotypes, and the ever-growing variety of recombinase systems. Picking Ligands Picking...

...originally described by Theodor Förster in 1948 as a variation of the more commonly observed light emission by...several excellent reviews have collated data from various papers to make the decision easier. 1. Müller, ... because the ratio of acceptor to donor fusions varies with transfection efficiency and any unpaired fluorescent...

...Polyclonal antibodies produced in animals may be variable among animals and bleed dates and even clonal ...CRISPR community has created hundreds of useful variants of Cas9. By providing access to the plasmids encoding...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080...

...noticeable influence on the outcome and cause variability in the testing group. For example, if an experimenter...distracted) from the ones tested at the beginning. Variability source Standardization parameters Animal Strain...This time window between injection and testing will vary depending on whether it is an overexpression gene...

...protocol [3] provides, in exquisite detail, the various steps required to produce your fiber optic implant...materials and methods of a paper that uses the variant you wish to use to determine the ideal excitation... be controlled. As discussed above, each opsin variant has slightly different activation/inactivation ...

...movement and is part of a bigger movement in a variety of research sectors to provide open source technologies...engineers, and biologists working together on a variety of projects including the OpenWorm project. The...model, and the biologists can get involved a in variety of aspects from the molecular biology to learning...

...the level of autofluorescence across various tissue types varies, it is critical to include an unstained... to avoid unmixing issues from batch-to-batch variation. Tandem dyes (two fluorescent molecules conjugated...

...versatility of Cas effectors allows for a wide variety of screens, including interference, activation,...using VSV-G. In contrast, AAV can be packaged in a variety of capsids. To date, at least 12 different capsids...the amount of cloning, another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog...

...The VSD components have historically come from a variety of sources – voltage-gated potassium channels found...Chemigenetic Sensors These types of sensors can have a variety of different components, but all contain a FRET...upon host infection. Packaging viruses also have various serotypes – the surface proteins that confer affinity...

...biology in our Plasmids 101 eBook! Researchers use various techniques to control protein expression for experimental...protein tags and separated from contaminants using a variety of purification methods. Deciding which expression...expression of recombinant proteins. Many cells from various types of plants such as maize, tobacco, rice, sugarcane...

...because different CAPTURE cell lines will have varying levels of dCas9 and gRNA expression. Additionally...CRISPR users Learn how to Choose the Best Cas9 Variant for Your Next Experiment Learn More About Designing...Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions of Interest Here...