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We narrowed to 772 results for: Vars;

Showing: 341 - 360 of 772 results

  1. To Each HIS Own

    Type
    Blog Post
    ...tens of thousands of proteins functioning in a variety of ways. How do scientists focus on the activity...acid sequence, or tag, on the protein. While a variety of methods can be used to successfully isolate ...tools for studying proteins and are used for a variety of assays in the lab (for a refresher see Antibodies...

  2. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ... is explained in more detail here. There are a variety of methods that can be used to measure your fluorescent...filtration (Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay ... Of course, you want the ‘latest and greatest’ variants with the best properties for your research. But...

  3. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...different fluorescence imaging strategies use widely varying amounts of excitation power (Shaner et al, 2008... An example of a photostability measurement of various cyan fluorescent proteins is depicted in figure...higher excitation power, the photostability of the variants is comparable (lower panel of figure 1). To avoid...

  4. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...from countless rounds of optimization. Dozens of variants exist with unique cutting features and evolved...engineering endeavors. For more information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme... detection tool for rare, often hard to detect variants such as single nucleotide polymorphisms. Fast...

  5. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...RNA-protein interactions. To address this need, the Khavari lab at Stanford created the RNA-protein interaction...disease. RaPID can also be used to test how RNA variants associated with human disease affect protein binding...Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction detection...

  6. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...for oxidizing environments to photoconvertible variants, it seems like GFP is always learning new things...lab researcher Jonathan Tang wanted to express a variety of proteins in single cell types in the mouse, ...proteins with a modular structure, constructing split variants should be relatively straightforward, but it is...

  9. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... and some, such as varenicline, are already approved for clinical use. Varenicline has been further modified...promoters, AAV serotypes, and the ever-growing variety of recombinase systems. Picking Ligands Picking...

  10. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...originally described by Theodor Förster in 1948 as a variation of the more commonly observed light emission by...several excellent reviews have collated data from various papers to make the decision easier. 1. Müller, ... because the ratio of acceptor to donor fusions varies with transfection efficiency and any unpaired fluorescent...

  11. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...protocol [3] provides, in exquisite detail, the various steps required to produce your fiber optic implant...materials and methods of a paper that uses the variant you wish to use to determine the ideal excitation... be controlled. As discussed above, each opsin variant has slightly different activation/inactivation ...

  12. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...movement and is part of a bigger movement in a variety of research sectors to provide open source technologies...engineers, and biologists working together on a variety of projects including the OpenWorm project. The...model, and the biologists can get involved a in variety of aspects from the molecular biology to learning...

  13. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...because different CAPTURE cell lines will have varying levels of dCas9 and gRNA expression. Additionally...CRISPR users Learn how to Choose the Best Cas9 Variant for Your Next Experiment Learn More About Designing...Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions of Interest Here...

  14. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...biology in our Plasmids 101 eBook! Researchers use various techniques to control protein expression for experimental...protein tags and separated from contaminants using a variety of purification methods. Deciding which expression...expression of recombinant proteins. Many cells from various types of plants such as maize, tobacco, rice, sugarcane...

  15. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...The VSD components have historically come from a variety of sources – voltage-gated potassium channels found...Chemigenetic Sensors These types of sensors can have a variety of different components, but all contain a FRET...upon host infection. Packaging viruses also have various serotypes – the surface proteins that confer affinity...

  16. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...the level of autofluorescence across various tissue types varies, it is critical to include an unstained... to avoid unmixing issues from batch-to-batch variation. Tandem dyes (two fluorescent molecules conjugated...

  17. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...versatility of Cas effectors allows for a wide variety of screens, including interference, activation,...using VSV-G. In contrast, AAV can be packaged in a variety of capsids. To date, at least 12 different capsids...the amount of cloning, another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog...

  18. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...volume = more cells = more plasmid. Plasmids can vary in copy number, the number of plasmid copies supported...plasmid DNA yield. Different plasmids and strains will vary in their optimal growth conditions. For many high...cell density or by harvesting and miniprepping at various time points. Measuring the optical density of the...

  19. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...discovered that there are many ways to get a taste of a variety of careers while still working towards your degree...graduate office arranged opportunities to meet with various professionals, and there were multiple avenues ... Find Plasmids for Your Research Learn About A Variety of Different Fields on Our Collections Pages ...

  20. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...Technology Development Fellow at the Wyss Institute and Harvard Medical School. Scientists making transgenic organisms...seeks to keep the organisms in the laboratory. It varies by organism, but your local biosafety officer should... cas9 gene). An immunizing reversal drive is a variant that also spreads through the wild population and...
Showing: 341 - 360 of 772 results