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...Resources at Addgene.org Find careers at Addgene Watch out career videos Sign up for the Addgene quarterly...making this transition. In this installment of the Addgene career guide, we’ll think about what causes this...job candidate for a senior scientist position at Addgene, the skeptical applicant asked if she would partake...of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list of things...the years that one of the things I enjoy about Addgene is that I’ve never been bored. There is always ...someone who coordinates cross-team projects at Addgene, I find that my vocabulary improves on a weekly...fix them, and take a look at these tips from the Addgene blog. You’ll have the opportunity to discover...

...Introduction to Antibodies Resources on Addgene.org  Browse Addgene's recombinant antibody plasmid collection...Addgene has recently announced the launch of an open-access recombinant antibody resource called NABOR...expressed from plasmids that have been deposited with Addgene. Here, we take a closer look at the construction...that will be the first antibodies distributed by Addgene.  The plasmids used to generate these antibodies...sequence. These plasmids have been deposited with Addgene for distribution to the scientific community with...affinity reagent tools to include nanobodies. At Addgene, we are excited to be part of this initiative and...References and Resources Additional Resources on the Addgene blog:  Plasmid-Based Recombinant Antibodies  Plasmids...

... available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids...been functionally tested by Ginkgo Bioworks or Addgene. If you used plasmids from this collection in your...cerevisiae expression vectors are available at Addgene. A set of mammalian expression vectors containing...COVID-19 Plasmid Collection Quality control at Addgene in the midst of COVID-19 We received Ginkgo’s first... our standard full plasmid sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids...screening and the use of high throughput platforms, Addgene and Ginkgo are striking a balance by modifying ...978-1-59745-181-9_23 Additional resources on the Addgene blog Read all of our COVID-19 blog posts Learn...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...Additional Resources from the Addgene Blog Visit our CRISPR featured topic page Check out our Hot Plasmids... addgene podcast...synthetic biology researcher Tom Ellis Resources at Addgene.org Find CRISPR Plasmids Find Synthetic Biology ...

... field. Resources at Addgene Browse Addgene's RNAi resources Browse Addgene's CRISPR resources Read ...countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic Acids Forum 2015 from Joanne...conference invited me to attend and speak about Addgene’s experience helping scientists share plasmids, ... talk to so many scientists who were potential Addgene requestors and depositors. China is in one of the...conference was kicked off by Nobel Laureate (and Addgene depositor) Craig Mello. Taking his groundbreaking...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...research and to keep useful discoveries coming. At Addgene, we’re huge supporters of science communication...Additional Resources on the Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about...

... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...but browsing the 49 new preps available in the Addgene viral vector repository with a nice cup of hot ...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...different serotypes. Not sure what to request? Addgene's Data Hub can help, with over 100 user-submitted... the link below to keep up with the latest in Addgene's viral vector services and resources!   This blog...

...contain thousands or millions of different books. Addgene’s pooled libraries are a little different: each ...are currently 390 pooled libraries available at Addgene, with more added to the collection all the time...technologies multiply, so do CRISPR pooled libraries! Addgene’s collection also includes a base editing sensor...we whetted your appetite for pooled libraries? Addgene’s pooled library collection page features hundreds...are offered. Figure 4: Screenshot of the Addgene website. Let us know which pooled libraries...comments! Resources Additional resources on the Addgene blog Viral Vectors 101: Preparing Pooled Libraries... Pooled Library Amplifications Resources on Addgene.org Browse all pooled libraries Guide to Plasmid...

... autophagy biosensors currently available from Addgene. Rosella autophagy biosensor Rosella is a dual-...-targeted and mitochondria-targeted Rosella at Addgene! Keima autophagy biosensor Keima is a mammalian...Find the mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy... red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe that releases... flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J., Ana Maria Cuervo...PMCID: PMC5618514. Additional resources on the Addgene blog Beware of fluorescent protein oligomerization... sheet fluorescence microscopy Resources on Addgene.org Fluorescent protein plasmids and resources Fluorescent...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Plasmid Rap Plasmid rap video made with support from Addgene.      Check out more science education tools...Additional Resources on the Addgene Blog Get Advice on How to Build a Grad Student Community Check out All...Plasmids 101 Series Additional Resources on Addgene.org Check out Our Protocol Pages for New Protocol...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Along these lines, Darrell recently joined the Addgene Board of Directors where he hopes to help keep ...Chapter 12: Darrell's goals as a member of the Addgene board of directors - increasing open source sharing...Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the...

...Resources on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s CRISPR 101 eBook...end goal. CasPEDIA also features useful links to Addgene resources throughout the site. In the Experimental...each enzyme page, you'll find a link to search Addgene's collection for plasmids containing that enzyme... from the article if they're available through Addgene. So whether you're looking for the exact Cas9 plasmid... or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered!     ... eBook More resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment...

...NBK19423/ Resources on Addgene.org Addgene's most popular lentiviral plasmids Addgene's most popular retroviral...retroviral plasmids Addgene's collection of AAV plasmids Addgene’s collection of adenovirus plasmids Resources...of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many...components of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for ...Resources on the Addgene blog Parts of the AAV packaging plasmid Overview of Lentiviral Packaging Plasmids...

...they grow. The top 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral...components over the years on their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes...2A-Puro (PX459) V2.0 was made available through Addgene in 2015, making this the most recently available...fact: published in 2003, this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd...shRNA sequences. For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more...projects in the years to come.  Resources on the Addgene blog Browse our CRISPR posts Read our viral vector...posts Read our lentivirus guide Resources on the Addgene website Browse our CRISPR collection Find plasmids...

... Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View our ...the best things about sharing plasmids through Addgene is that we provide an added level of confidence... plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid we distribute...physical sample and its associated information are at Addgene, we are ready to sequence verify the plasmids. ...plasmid you are working with, but, in most cases at Addgene, we check what we call the “insert,” (the gene ...comments section below.  Additional Resources on the Addgene Blog Get 6 Tips for Analyzing and Troubleshooting...

...Marillonnet share with the scientific community via Addgene, the struggles of plant scientists and how they...Everybody's favorite parts for plant synthetic biology Addgene: Tell me about the Golden Gate Modular Cloning ...elements, which has not been available for plants. Addgene: Will the MoClo tools work in any plant? Patron... it a good laboratory workhorse. Tips & tricks Addgene: Do you have any particular tips for those looking...start and go ahead. I can see science speeding up. Addgene: Are there other tools you are working on for plants...interaction work and we’ve gotten some tools back from Addgene recently to explore their use in plants, including...technologies in the near future. Science on Twitter Addgene: You are active on twitter @nicolabiologist, where...

...about the ClonTracr Library Resources on Addgene.org Addgene's most popular lentiviral plasmids Overview...lentiviruses can infect dividing and non-dividing cells.  Addgene has an extensive collection of lentiviral plasmids...lentiviral questions. This useful resource from Addgene Depositor (and lentiviral expert) Didier Trono'...gritty and answer the questions those of us at Addgene get asked most often: Q1: Are retroviruses and ...and 3rd-generation transfer vectors apart? A3: Addgene defines 2nd- or 3rd-generation transfer vectors... competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are replication-deficient meaning...Trono Lab: Lentivectors Toolbox Resources on the Addgene Blog: Download our eBook: Plasmids 101 Learn about...

... Resources on Addgene.org Find plasmids for your research Deposit plasmids at Addgene  ...Addgene recently moved to our brand new location, with more space to grow and help scientists share resources...Gene names! This is not surprising for Addgene. But the names we chose were not just any gene names. Now,...embryonic development The largest conference room at Addgene is named Sonic Hedgehog but this is not a direct...divide properly into the right and left hemisphere. Addgene has several plasmids that contain SHH, you can ...the stomachs of different animals. Check out the Addgene website for plasmids that contain tinman or the...mammalian homolog Nkx2.5. We also have one plasmid, Addgene plasmid 34260, that contains bagpipe from the Deplancke...

...Golden Gate Cloning Additional resources on addgene.org Addgene's MoClo Guide and Kits MoClo Toolkit MoClo...chosen one of the MoClo kits available through Addgene that suits your experimental purposes. But what...park.  Before you begin, be sure to check out Addgene’s MoClo guide for all the basics of the process....coding sequence of interestCDS Level 0 part from an Addgene MoClo kit, to construct a Level 1 vector with a...the plasmid sequence files provided with every Addgene MoClo kit through their online catalog pages. We...your MoClo kit has just arrived! MoClo kits from Addgene will most likely arrive in your laboratory as plates...recommend retrieving plasmids from your original Addgene kit plate as few times as possible and always under...

...month including a first of its kind for the Addgene blog - Addgene Software Engineer Kris Shamloo wrote a ... Read All Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the...constructs. The second post was contributed by Addgene Scientist Mary Gearing and discusses a newer, CRISPR-based...advice on how to give a great science talk from Addgene Executive Director Joanne Kamens. I highly recommend...