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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 648 results for: ato

Showing: 341 - 360 of 648 results

  1. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...nicking/cleaving of DNA occurs, not for deadCas activator/repressor experiments where only localization ...RNA-seq would be an appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would...

  2. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using...10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender...

  5. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...DNA transposons are most commonly used in the laboratory for genome manipulation. When transposons are...2020) Jump around: transposons in and out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research...

  6. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, also expressed by your transfer vector...

  7. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...

  8. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ... unbiased discovery. With these screens, the investigator generates a cell population where all genes ...contexts. Most of the immune cells we study are migratory, and their exact localization is important for...

  9. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...absorbed by matter, they promote electrons within atoms to a higher energy state. This process is called.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

  10. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...environment? If these are pressing issues in your laboratory, then FRET experiments are a viable biophysical...and purification is a snap using thin layer chromatography (Fessenden, 2009). However, these reagents ...

  11. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... electrophysiology data too but that’s what collaborators are for). For example, if you study neural pathways...projection-specific neuronal populations using combinatorial viral approaches. Current protocols in neuroscience...

  13. Zinc Finger Consortium Reagents

    Type
    Collection
    ...proteins for distribution to academic and nonprofit laboratories for research and educational use. Links to additional...

  14. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...any applications. DNA quantity is fairly self explanatory and achieved by performing different sized preps...about!) are a significant source of waste in laboratories. To help combat this, some manufacturers have...

  15. KLF Research Plasmids

    Type
    Collection
    ...family of at least 17 DNA binding transcriptional regulators with diverse roles in processes such as cell ...

  17. Fluorescent Protein Guide

    Type
    Collection
    ...signals, cells, or groups of cells with either actuators or sensors. Chemogenetics Plasmids | Guide Use...

  18. AAV Viral Preps

    Type
    Collection
    ...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation, CRISPR...
Showing: 341 - 360 of 648 results