We narrowed to 376 results for: bre

1. Viral Vectors 101: Preparing Pooled Libraries

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   Blog Post

   ...incubation time, antibiotic conditions, and amount of polybrene. (psst…didn’t get your library pre-made from Addgene...

2. Plasmids 101: Stringent Regulation of Replication

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   Blog Post

   ...strands. Created with BioRender.com. Once DnaA breaks the local hydrogen bonds holding the DNA together...

3. Plasmids 101: Broad Host Range Plasmids

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   Blog Post

   ...Alcaligenes eutrophus, Azotobacter vinelandii, Brevibacterium methylicum, Caulobacter crescentus, Desulfovibrio...

4. Antibodies 101: Reading a Flow Plot

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   Blog Post

   ...first… which can send you in an endless loop. Let’s break out of the loop with all you need to know on how...

5. Analyzing CRISPR Editing Results with ICE from Synthego

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   Blog Post

   ...single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added to the...

6. Antibiotic Resistance: An Old Solution but a New Problem

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   Blog Post

   ..., we can all work together to try and prevent outbreaks of drug resistant bacteria within communities,...

7. The Challenges of Cell Culture

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   Blog Post

   ...2002): 315-319. PubMed PMID: 12001993. 4. Lucey, Brendan P., Walter A. Nelson-Rees, and Grover M. Hutchins...

8. Getting the Most from Your Lentiviral Transduction

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   Blog Post

   ...typically employ the positively charged polycation, polybrene. Interestingly, one study found that alternative...

9. Important Considerations in Optogenetics Behavioral Experiments

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   Blog Post

   ...behavioral effect but the function of specific subregions within the hippocampus were only recently studied...

10. Molecular Biology Reference

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    Guide

    ...bases, adenine, thymine, cytosine, and guanine (abbreviated to A, T, C, and G respectively) that are organized...process of molecular cloning enabled scientists to break chromosomes down to study their genes, marking the...series covers topics ranging from the newest breakthroughs in plasmid technologies and research, to overviews...

11. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post

    ...South Korea, one of the earlier epicenters of the outbreak ultimately flattening the curve, it has become...

12. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post

    ...and CiteAb. The antibody you choose can make or break your experiment, so take advantage of all the available...

13. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post

    ...transfection or viral delivery of a plasmid. RNAi is an umbrella term for a number of different applications that...

14. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    Blog Post

    ...is very efficient at introducing double stranded breaks (DSBs) into genomic DNA in many cell types and ...

15. March for Science

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    Blog Post

    ...cuts (3, 12, 13, 27). In order to achieve the breakthroughs, technologies, and treatments promised by modern...

16. Evolution of Lab Techniques

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    Blog Post

    ...chain-terminating or dideoxy sequencing, was a major breakthrough for determining the order of nucleotides in ...

17. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post

    ...the basics of Gibson assembly as a parody of “Breakfast at Tiffany’s.” Overview of Gibson assembly The...

18. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post

    ...distinct cell populations. To decrease the animal breeding necessary to produce animals with Brainbow transgenes...

19. Viral Vectors 101: AAV Variables That Matter

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    Blog Post

    ...., Hu, H., Lee, Y., D’Azzo, A., Messing, A., & Brenner, M. (2004). Expression specificity of GFAP transgenes...

20. Hot Plasmids: Spring 2025

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    Blog Post

    ...resistance gene makes stable cell line creation a breeze. Modular by design: Key elements (promoter, tag...