We narrowed to 660 results for: epo

...may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either... well of your cells. Regardless, the amount of reporter protein activity correlates to both the amount...

...SynBio Genome Editing Gene Silencing Reporters Addgene’s repository contains many plasmid tools that were...Return to top Reporter Plasmids The table below highlights a selection of popular reporter plasmids that...plant-optimized plasmids for cloning, gene expression, reporter assays, genome editing, gene silencing, and more...from genome engineering and gene expression to reporter systems. Addgene’s collection includes plasmids...The following plasmid kits contain a variety of reporter plasmids for the study of protein–protein interactions... Golden Gate (MoClo) Benjamin Field Additional Reporter Plasmid Resources Visit our Fluorescent Protein...

...impressive on a larger scale, too. The first paper reporting genome editing by CRISPR in mammalian cells appeared...reviewed in Lander, 2016). The first base editor was reported three years later in 2016 (Komor et al., 2016)...

...interest with an additional protein module that can report on location. This approach enables the study of...9759496.  3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence imaging." Current opinion in ...

...discovery of RNAi in the worm actually rewarded depositors Andrew Fire and Craig Mello a Nobel Prize in ...important gene co‑expression modules." Biomedical Reports 7.2 (2017): 153-158. PubMed PMID: 28804628. PubMed...

... the Confetti mouse (Snippert et al., 2010), a reporter mouse that can be used to track any cell type ...XFP expression in the Ubow mouse Another murine reporter animal, the Ubow mouse (Ghigo et al., 2013), was...

... where two proteins are fused to complementary reporter proteins that can only assemble when in close ... ascorbate peroxidase as a genetically encoded reporter for electron microscopy." Nature biotechnology...

...great point when I then ask “and who has deposited to the repository?” When most or all of the hands go down...

...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with SARS-CoV-...44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase...Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin... pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection. Dapeng...

...luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid...expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and ...for viral vector delivery, genome modification, reporter assays, shRNA expression, transgenics, and more...page for more information Genome Modifications, Reporter Assays, mRNA Regulation, and More Find empty backbones...backbones for other uses such as genome modification, reporter assays, shRNA expression, transgenics, and more...TALEN array for genomic engineering Luciferase Reporter pGL3 Basic Vector - Firefly luciferase vector...genes can be inserted into this firefly luciferase reporter to test for their effects on protein production...

...Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45 - Reagent Development - Steven...of reproducibility for preclinical studies, as reported by drug companies attempting to apply the findings...

... it a single nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with... R (2013) One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated...

...distributed throughout the genome. We encourage you to deposit your piggyBac™ transposon vectors with us to help... up on Everything CRISPR with Our CRISPR Guide Deposit Your piggyBac™ Vectors! ...

...piggyBac plasmids    Tol2  Tol2 was the first reported active DNA transposon in vertebrates. It was discovered...mutations. In these screens, the transposons encode reporter genes, mutagenic cassettes, or barcodes. When ...

...gene (e.g., channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing...recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed PMID: 29743652. PubMed...

... unbiased methods, high background BLESS Not reported Cell-based (in vitro) Can be used on tissue from...senstive to time since cell fixing HTGTS Not reported Cell-based Identifies translocations Limited by...

... aren't in the repository? Help us widen our resources for the community by depositing your useful tools...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial...bacteria, and more. Synthetic Biology : Browse our deposited tools and resources, or read our guide on assembly... reference page for CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast...Genome Engineering blog post to see how Addgene depositors are making this possible in a range of organisms...

... self-labeling tools: Tetracysteine: The first reported chemical labeling tag, from Roger Tsien’s lab ...collection. CLIP-tag: Also in 2008, Kai Johnsson’s lab reported another AGT-based tag named CLIP-tag, which reacts...

...transgenic mice expressing fluorescent protein reporter constructs in the immune lineage. By specifically...using 2-photon microscopy as this hasn't been well reported for many fluorescent proteins. Additional fluorescent...

...The Eric Mendenhall and Richard Myers labs have deposited plasmids for a CRISPR-based system to add a tag...homology arms and selection marker. The first deposited plasmids in this CRISPR-Cas tagging system were... Tagging System Recently, Yannick Doyon's lab deposited a plasmid which introduces an N- or C- terminal...PITCh Tagging System The Takashi Yamamoto lab has deposited the PITCh system plasmids for CRISPR-based knock-in...microhomologous sequences and gRNA target sites. The first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ...