We narrowed to 622 results for: erf

... the software space but generally rely on less powerful algorithms to detect off-targets. The few offline...offline tool, but comes with a graphical user interface. Best strategy for labs: Define what you need ...

...most machines have a protocol that automatically performs the normalization step.  Machines Most general-use...the results are correct. Primers and probes can interfere with amplification reactions, and reagents can...

...combinations to optimize both fusion proteins. The best performing combination was NLS-dCas9-trCIB1 and NLSx3-CRYPHR-p65...AcrIIA4, this unfolding conformational shift can interfere with activity. The resulting system was coined...

...of viral transgenes in iPSC-derived cells can interfere with their differentiation potential. Finally,...to purify the reprogramming proteins needed to perform these experiments. Assays for iPSCs Characterization...

...different than the validation assays, we recommend performing your own validation assays. If you’re using previously...interest is not available in a pure form, you can perform relative quantification, which measures protein...

...antibiotics (no penicillin or streptomycin). Day 2: b. Perform the transfection in the late afternoon because ...

...thought to yourself, “Oh! This plasmid would be perfect for my next experiment!” but were unable to find...

... day of CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth...

...CRISPR, Addgene gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd...

...deposits from Arlene Sharpe’s lab. X-Chime and Performing In Vivo CRISPR Screens Using the FITS Approach...

...Kuhlman and Dantas et al., 2003). That made it a powerful proof of concept that this kind of protein design...

...our brand new Addgene Videos page! Videos are a powerful tool for communicating scientific knowledge. On...

...VSV-G envelope protein under the control of the powerful mammalian CMV promoter. Envelope proteins are ...

...unloading of the column and give some tips for performing this purification.  Check out the AAV Purification...

...Falcon tube trees to giant paper mache Krampus performing routine lab maintenance tasks. Hot tip: make ...

... what type of tissue culture dish you will be performing the transfection in: 15 cm dishes OR CF5 stacks...

... available from Addgene, he knew it would be a perfect fit for the streak plate challenge. Chromoproteins...

...had our yearly Halloween party (see all of our wonderful costumes in the pictures below, yours truly is...

... biology students about the functions of these powerful tools and to use them as platforms for the construction...

...characteristic allows monoclonal antibodies to be used as a powerful reagent in the laboratory.   The DSHB focuses ...