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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 636 results for: erf

Showing: 341 - 360 of 636 results

  1. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...caught the attention of researchers wanting to perform whole-animal imaging, however NLuc’s emission peak...higher the emission from the acceptor, the more powerful the FRET signal. In well-designed FRET-based biosensors...

  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...ever both to engineer specific DNA edits and to perform functional screens to identify genes involved in...chromatin status). No computational prediction is ever perfect, but this can decrease the number of guides one...

  3. A Control for All Seasons

    Type
    Blog Post
    ...extremely bright, then the noise likely will not interfere. If the fluorescence from your target is weak,...fluorescence of the sample, or autofluorescence, also interferes with data interpretation and can be addressed...

  4. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...more.  Conclusion   Optogenetic tools provide a powerful way to study multiple aspects of cellular activity...important to understand the details of how each tool performs and the consequences of off-target activity. Whether...

  5. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... iGluSnFR3 mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation) and...Meghan Rego You asked, we listened! One of the most powerful tools in a scientist's toolkit is the empty plasmid...

  6. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...polymerase is a phage-derived polymerase that can perform whole genome amplification (by a mechanism known...below). This initial transformation should be performed using a highly competent strain that you can use...

  7. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...produce completely transgenic offspring. Although powerful, this technology is cumbersome to use, not that...concept, straightforward to use, and robust in performance. In its natural setting, CRISPR-Cas9 is an acquired...

  8. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... questions one could pursue and experiments to perform. It is tempting to pursue a new line of inquiry... school student. Learn to teach how to design, perform, and interpret experiments. You'll be better prepared...

  9. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...for guiding new deposits into the repository, performing quality control, and providing technical customer...strong sense of purpose, an excellent leader, and perfectly clear lines of communication, but if teammates...

  11. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...link genotype and phenotype. It is also key to perform these validations as translational researchers ...the Addgene Blog Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse ...

  12. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...restriction enzymes (Weber et al., 2011). It can be performed at multiple levels to generate anything from individual... tags, connectors, cDNAs, and more to find the perfect combination. The EcoFlex Kit has a wide array of...

  14. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...these types of experiments easier and faster to perform. Introduction to flow cytometry Flow cytometry ...containing a negative cell is discarded. FACS can be performed aseptically allowing users to culture the cells...

  16. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...quantity is fairly self explanatory and achieved by performing different sized preps, such as miniprep, midiprep...bead- and column-based DNA purification methods perform well in terms of quality and yield. Bead-based ...

  17. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...hypothesized that the adenosine deaminase could also perform cytidine (C) deamination, converting the C base...uracil (U). This is the same chemical reaction performed by CBEs. However, most CBEs include an inhibitor...

  19. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...of viral transgenes in iPSC-derived cells can interfere with their differentiation potential. Finally,...to purify the reprogramming proteins needed to perform these experiments. Assays for iPSCs Characterization...

  20. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...combinations to optimize both fusion proteins. The best performing combination was NLS-dCas9-trCIB1 and NLSx3-CRYPHR-p65...AcrIIA4, this unfolding conformational shift can interfere with activity. The resulting system was coined...
Showing: 341 - 360 of 636 results