Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 709 results for: esc

Showing: 341 - 360 of 709 results

  1. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...immediately write a text document with a brief description of the directory contents, at a minimum. This...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...

  6. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...talking about here? Many of the Fab fragments described above can be used for similar applications and...tissue-specific molecules identified by a double-label immunofluorescence technique for monoclonal antibodies.  J Histochem...

  7. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined...

  8. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ..., non-dividing cells.   Figure 2: DNA rescue and transduction of a conventional single-stranded...Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually packaged...

  9. All in a Twist: dsRNA

    Type
    Blog Post
    ...prone to degradation – those are the words that describe RNA! Double stranded? Well, that’s just for DNA... and repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular...

  10. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...simple LED light. Lighting up your gRNA with fluorescent dyes In a scientific world where it often seems...seems like everything glows, why not spread the fluorescence to your gRNAs? Dye labeling the RNA in your ...

  15. CRISPR in the Clinic

    Type
    Blog Post
    ...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...They found that the cataracts phenotype could be rescued by zygote co-injection of Cas9 and a single-guide...

  17. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...visualize proteins in vivo by tagging them with fluorescent proteins to study localization or purify proteins...Expression Systems Recombinant protein expression in Escherichia coli: advances and challenges Production of recombinant...

  20. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...Building on the lab’s previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in... transgenes. It’s important to note that the fluorescence level observed will be dependent on both the...
Showing: 341 - 360 of 709 results