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  2. Lab Automation at Addgene

    Type
    Blog Post
    ...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...

  4. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...mammalian cell lines, Drosophila cells, and C. elegans. In addition to generating fluorescent protein .../Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional Resources...

  5. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...Mice are a common model organism used to understand mammalian traits and genetically engineered mouse...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor alpha (Esr1...

  6. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...

  7. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...Green. Zika background First identified in 1947 in Uganda, the Zika virus had received little attention and...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...

  9. Transferable Skills: Negotiation

    Type
    Blog Post
    ...with your colleagues within and outside of your organization. In my non-academic roles, some examples of ...development analyst, I negotiate with global organizations that partner with Addgene. What are the most...

  14. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc., which will...target protein and an E3 ligase through interacting ligands. After an E3 ligase facilitates ubiquitination ...

  15. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ...3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal cords...

  17. Which Fluorescent Protein Should I Use?

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    Blog Post
    ...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...

  18. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...figure. Some follow the same figure by figure organization with a different person responsible for each...journal clubs should be well planned and carefully organized. Most importantly, they should be regularly assessed...

  20. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ..., which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene... Percent of Neurons Transduced   Dorsal Root Ganglion (DRG) AAV.9 (parental) 46 +/- 0.7% AAV.PHP.S...
Showing: 341 - 360 of 607 results