We narrowed to 445 results for: hal

...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

...finding that uninterrupted time to be creative is challenging,” says Susan.  Another difficulty is that some...

... with Cas9 guided by different promoters. The challenge in building genetic circuits is getting enough...

...in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology,...

...in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. ...

...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...

...students (1, 2), but educators face significant challenges in designing suitable projects. In the O'Connor...

...codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes a ...

... make it difficult to detect proteins with short half-lives. Interested in using Split-BioID with your...

...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...

...pronucleus of the zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades...

...the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects...

... is only ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is in part due to the fact...

...Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church ...

...that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. ...

...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

... (budding yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic...

...symptoms, including the development of fetal microcephaly and Guillain-Barré syndrome. In response, the...

...validation experiments we discussed last week are only half of the battle when using optogentics to answer a...

... case.   Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...