Addgene

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Showing: 341 - 360 of 430 results

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Adenoviral Vector Production and Troubleshooting

Type

Blog Post

...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...
Pathways Over Time Plasmids Engage Students in Functional Genomics Research

Type

Blog Post

...students (1, 2), but educators face significant challenges in designing suitable projects. In the O'Connor...
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Type

Blog Post

...codon-optimized cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes a ...
Split-BioID: An Improved Method for Studying Protein-Protein Interactions

Type

Blog Post

... make it difficult to detect proteins with short half-lives. Interested in using Split-BioID with your...
Plasmids for Endogenous Gene Tagging in Human Cells

Type

Blog Post

...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Type

Blog Post

... (budding yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic...
Adapting Toehold Switches to Detect Zika Virus

Type

Blog Post

...symptoms, including the development of fetal microcephaly and Guillain-Barré syndrome. In response, the...
The Materials Science of Optogenetics Experiments

Type

Blog Post

...validation experiments we discussed last week are only half of the battle when using optogentics to answer a...
Experimenting in an Open Source lab: from CRISPR to Cats

Type

Blog Post

... case. Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...
Hot Plasmids: Winter 2025

Type

Blog Post

... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....
The AAVantages of AAV in CRISPR Screens

Type

Blog Post

.... V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J...
Production of Virus in Insect Versus Mammalian Cells

Type

Blog Post

...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...
Antibodies 101: Flow Compensation

Type

Blog Post

...Compensating between your fluorescent markers can be challenging! But we hope this guide can help you get started...
Twenty Years of Addgene Sharing: CRISPR

Type

Blog Post

... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...
Of Myc and Men

Type

Blog Post

...in these species, they found that the N-terminal half of the antigen differs significantly between human...
PCR Applications for Copy Number Variation Assays

Type

Blog Post

...available, ddPCR may be a better option for more challenging cases. Price point If budget is a concern...
Polymerase Chain Reaction Overview and Applications

Type

Blog Post

...standard curve, which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion...
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

Type

Blog Post

...for instance in inherited disorders such as B-thalassemia, Sickle Cells Disease, Duchenne Muscular Dystrophy...
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Type

Blog Post

...access to routinely, and it can be especially challenging when trying to develop antibodies against unstable...
Academic vs. Industry Postdocs

Type

Blog Post

...in-depth discussion about your project might be challenging. In an academic environment, surrounded by lab...

Showing: 341 - 360 of 430 results

Results per page:

Adenoviral Vector Production and Troubleshooting

Pathways Over Time Plasmids Engage Students in Functional Genomics Research

Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Split-BioID: An Improved Method for Studying Protein-Protein Interactions

Plasmids for Endogenous Gene Tagging in Human Cells

Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Adapting Toehold Switches to Detect Zika Virus

The Materials Science of Optogenetics Experiments

Experimenting in an Open Source lab: from CRISPR to Cats

Hot Plasmids: Winter 2025

The AAVantages of AAV in CRISPR Screens

Production of Virus in Insect Versus Mammalian Cells

Antibodies 101: Flow Compensation

Twenty Years of Addgene Sharing: CRISPR

Of Myc and Men

PCR Applications for Copy Number Variation Assays

Polymerase Chain Reaction Overview and Applications

Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Academic vs. Industry Postdocs

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