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We narrowed to 850 results for: met

Showing: 341 - 360 of 850 results

  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...concentration. Three methods are broadly used for virus titration: the p24 ELISA dosing method detects the expression...expression of viral capsid antigen p24, the FACS dosing method correlates viral titre to the number of cells transduced... uses qPCR to quantify viral RNA. All of these methods have been described in a previous Addgene blog ...with the virus in order to aid viral binding. This method, called spinoculation, has been found very effective...

  2. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...commentary about the new work published in Cell Metabolism. But, they note, there is plenty more to explore...be important to identify the key proteins and metabolites that are dynamically secreted by muscle during... Skeletal muscle PGC-1α1 modulates kynurenine metabolism and mediates resilience to stress-induced depression...Muscle over mind. Moon HY, van Praag H. doi: 0.1016/j.cmet.2014.09.012. PMID: 25295784 Related Materials:...

  3. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...breakthrough in cloning technology with the Golden Gate method. For the first time, it was possible to quickly...tedious and time-consuming traditional cloning methods, Lohmann said. The key component of Golden Gate...their lab learned the hard way after the cloning method, which had been humming along at 99 percent efficiency... et al. "A One Pot, One Step, Precision Cloning Method with High Throughput Capability." PLoS ONE. 3(11...

  4. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...protein labeling with trimethoprim conjugates: A flexible chemical tag. Nature Methods, 2(4), Article 4. ... Cohen lab used HaloTag for their "ticker tape" method for intracellular recordings, switching between...H., Vogel, H., & Johnsson, K. (2003). A general method for the covalent labeling of fusion proteins with.... https://doi.org/10.1038/nmeth749  Strebinger, D., Frangieh, C. J., Friedrich, M. J., Faure, G., Macrae...

  5. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nature Methods, 14(10), ...technically cell-permeable, its use in live cells can sometimes result in cellular toxicity due to the permeation...guide to small-molecule fluorescent labels. Nature Methods, 19(2), 149–158. https://doi.org/10.1038/s41592..., 987–994. https://doi.org/10.1038/nmeth.4403  Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H.,...

  6. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...In complex metazoans, rapid cell division and large scale cell mobility are essential processes during...specificity or from the thymus to the lymph nodes. These methods gave researchers much more information regarding...allows imaging of living tissue up to about one millimeter in depth. Two low-energy photons (typically from... between several different immune cells. These methods allowed immunologists to develop a spatiotemporal...absorption properties of fluorescent proteins." Nature methods 8.5 (2011): 393-399. PubMed PMID: 21527931. PubMed...

  7. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...common method for checking both quantity and quality of your plasmid prep is by spectrophotometry. Achieving...one you are most familiar with. And, hey — if something is working for you, then there is no reason to...  Both bead- and column-based DNA purification methods perform well in terms of quality and yield. Bead-based...purification kit options Manufacturer DNA Isolation Method Kit Sizes Available Standard Miniprep Cost Reagents...

  8. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...world of academia often emphasizes structured methodologies and rigorous research plans. However, amidst...in nature or visiting museums can be excellent methods to detach from the constant stream of thoughts ...PhD studies, where every aspect of research is meticulously planned, it's easy to get stuck in your everyday...of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with...

  9. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...analysis in Drosophila. Nature Methods 9:47–55 . https://doi.org/10.1038/nmeth.1800 Isaacman-Beck J, Paik ...Maimon G, Wilson RI, Clandinin TR (2019) SPARC: a method to genetically manipulate precise proportions of...-wide reconstruction of single neurons. Nature Methods 15:1033–1036 . https://doi.org/10.1038/s41592-018...

  10. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...relevant metadata and the screen results. Screenshots updated 2024. Importantly, metadata for each...to screen result interpretation. The analytical method for score assignment and significance thresholds...gene hits for each screen are also captured as metadata.   Screen results are reported as the original...

  11. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...hormones strigolactones By Fabienne Furt Current methods used to detect strigolactones (SLs) are costly,...that of the internal control (Fig 1). Because this method relies on the fluorescence ratio between the 2 ...approach was also used to build and validate a ratiometric fluorescent biosensor based on the parasitic ...CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene.  CRISPR-Act3.0, ...

  12. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...sense dopamine. Methods for measuring Dopamine In vivo microdialysis The gold standard method of measuring... liquid for quantitation. The advantage of this method is that it can be performed in a live awake animal...the probe into a specific brain region. While the method is powerful and offers immediate measurements in...

  13. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...that was developed by Li, et al., in 2011. This method is ligation independent, it does not require purification...and then combine the products and digest with methylation sensitive restriction enzyme DpnI to remove the... Note—templates must have been isolated from methylation competent E. coli for this procedure to work ..., sequence-and ligation-independent PCR cloning method." BMC biotechnology 11.1 (2011): 1. PubMed PMID...

  15. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ... substrates inside the lysosome. Historically, methods for studying autophagy focused on counting the ...Daniel J., Ana Maria Cuervo, and Per O. Seglen. "Methods for monitoring autophagy from yeast to human." ..., Noboru, Tamotsu Yoshimori, and Beth Levine. "Methods in mammalian autophagy research." Cell 140.3 (2010...quantitative Pho8Δ60 assay of nonspecific autophagy." Methods in enzymology 451 (2008): 33-42. PubMed PMID: 19185711...

  16. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...your tables and figures. Now it's time to write. Sometimes, it's hard to get those words out and onto the..., you can take a longer 15-30 minute break. The method is named for the tomato or “Pomodoro” kitchen timer...manuscript they might be found, i.e. introduction, methods, results; or phrases are categorized by the goal...This podcast talks more about using the Pomodoro method in grad school Additional resources on the Addgene...

  17. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ... a high throughput manner. She helped develop a method that measures the effects of sequence variants ... the body’s response to warfarin. “These novel methods will be instrumental in interpreting human variation...sensor can also kinetically outperform current methods. Unger plans to use the sensor to answer the question...Pantoea agglomerans interaction. But the shape and metabolism of leaves can be changed by light and temperature...

  18. Hot Plasmids - January 2023

    Type
    Blog Post
    ...through our hot plasmids articles. Think we missed something smokin'? You can pitch a Hot Plasmids section ...of our most commonly requested antibodies. But sometimes, one antibody per target just isn’t enough and...agents. Adapted from Fig. 2 Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C.,...fluorescent proteins for advanced microscopy. Nat Methods 19, 1612–1621 (2022). https://doi.org/10.1038/s41592...

  19. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ... or good - they're just exist and sometimes we benefit and sometimes we don't."     - Jennifer Tsang, ...age) fight in the Napoleonic Wars and against something even more sinister. 1000 pages long. Incredibly...International Finance. A book club could lead to smart, sometimes contentious, discussions. I presented the first...

  20. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...taken up the plasmid in the same way. Selection methods, described below, are typically employed when creating...direct test for your gene of interest may be another method to assess transfection success. GFP is often used... a problem depending on the duration or other parameters of your experiment. Mammalian cells double at..., but still want to use transient transfection methods. Since there are no "natural" mammalian ORIs, scientists...
Showing: 341 - 360 of 850 results