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We narrowed to 779 results for: mos

Showing: 341 - 360 of 779 results

  1. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...the world encountered COVID-19 at different times. Most notably, China was affected by COVID-19 earlier ...placing orders per day across regions, with Europe most impacted and China least impacted. The inferred ... China COVID-19 date range is, they could be the most impacted or least impacted. These results highlight...

  2. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...response. At the time, adenoviral vectors were the most commonly used viral vectors for gene delivery, and...limitation is the small size of its DNA cargo - 4.8 kb at most - which means that a gene larger than 3 kb cannot... is back, better and safer. It is now one of the most active and exciting areas of science. This is hopefully...

  3. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... we’ll break down the mechanism of action of the most common antibiotic resistance genes and which applications...bacteria that express the Tn5 element, but thankfully, most competent cells do not express this factor. Zeocin...?  Works for multiple organisms? Compatible with most bacteria? No satellite colonies? Ampicillin Fair...

  4. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus...packaging and envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles with the...of research impact and safety. Many of Addgene’s most requested plasmids are used with lentiviral expression...

  5. CRISPR Antimicrobials

    Type
    Blog Post
    ....com.   The problem of antibiotic resistance Most antibiotics target one of three essential processes...are easy to see. As discussed in this blog post, most bacteria have poor non-homologous end joining machinery...specificity - rather than targeting a process essential to most bacteria, custom CRISPR antimicrobials can target...

  6. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...engineering tool. It is easy to use, functional in most species, and has many applications. That said, SpCas9...terms of structure, Cas13a shares no homology to the most commonly used CRISPR enzymes - Cas13a contains two...specifically targeted RNAs. Finally (and perhaps most interesting), Cas13a’s propensity to cleave any ...

  7. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...fluorescent visualization techniques for decades, most notably for fluorescent in situ hybridization (FISH...readout may render this approach less sensitive in most applications. Learn About Fluorescent Biosensors...Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc. Natl. Acad. Sci. ...

  8. How to Write a Scientific Review Article

    Type
    Blog Post
    ...block of time was. Eventually, I learned that I was most productive in short bursts (even twenty minutes ...minutes was enough to make some progress). For most researchers, it’s not always possible to pull a Thoreau ...useful data Typically lists and pictures are the most useful parts of reviews. These could be in the form...

  9. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ... exist for reducing Cas9 off-target effects, but most studies have focused on the off-target cutting of...not associated with ricin regulation, including almost all genes involved in the production of a cell ...fail to identify some known ricin-regulators, but most of these genes are also essential for growth and...

  10. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...much easier to map these neuronal networks. For almost three decades, recombinant Adeno-associated virus...IHC) experiments with tag-specific antibodies. The most famous ones are influenza hemagglutinin (HA), myelocytomatosis... microscopes, but they can also be used with the most cutting-edge imaging technologies such as array ...

  11. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...markers are set up sequentially, from broadest to most specific marker, and are represented as a series...quadrant will often be too clean for real-world data. Most plots will have a shape, like a box, ellipse, or...  As the gates progress from broadest markers to most specific, the first step is to separate cells from...

  12. How to Be an Excellent Trainee

    Type
    Blog Post
    ...your best to show up as your most prepared, can-do self and make the most of it! So, what can you do ...dynamic and set yourself up for success! Getting the most out of the training experience In research there...

  13. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...Planning makes perfect Our first tip, and arguably the most important, is to take some time to sit down, plan...assemblies in silico before you start doing your cloning. Most sequence analysis software includes Golden Gate ...kit has just arrived! MoClo kits from Addgene will most likely arrive in your laboratory as plates of frozen...

  14. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...adenine transversion editors Uracil excision is the most common type of base excision repair. But mammals... retained considerable A → G editing activity at most sites, in addition to A → C and A → T activity. ... base from DNA to create an abasic site. Like in most other base editor designs, the Cas9 nicks the non-edited...

  16. Lab to Office Culture Shock

    Type
    Blog Post
    ... just creating, which for many scientists is the most enjoyable aspect of their chosen career. You can...’t know what it’s like to be properly onboarded. Most work environments outside of academia provide a ...skills you pick up wherever you work, and you’ve almost certainly developed some in the lab whether you...

  17. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ... is reinforcing, the animal will prefer to spend most of its time in the chamber in which it receives ...conversely, if the circuit is aversive, it will spend most of its times in the opposite chamber) [2]. Laser...standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this species. However...

  18. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ... the company. I’d identified the ways to get the most out the resources we have, and we developed a great...But there were still many restrictions in place, most importantly, masks.  Besides the muffled voices ....  Test: Our first official shoot went off with almost no hitches. The wheeled stand was a bit cumbersome...

  19. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...types and often results in biallelic modifications. Most commonly, DSBs are repaired by the nonhomologous...associated with dementia, such as APP and PSEN1, in most cases the intended HDR events were corrupted by ...’t get what we wanted. The Indels were gone from most HDR reads that we studied by deep sequencing, but...

  20. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...Microscopy (University of Amsterdam). GFP is the most popular, most widely used genetically encoded fluorescent...maturation to functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add...
Showing: 341 - 360 of 779 results