We narrowed to 398 results for: nam

1. Cpf1: A New Tool for CRISPR Genome Editing

   Type

   Blog Post

   ...potentially overcome some of Cas9’s shortcomings - namely its blunt double stranded cleavage and G-rich PAM...

2. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   Blog Post

   ...engineered DNA-binding molecules for the first time and named the new technology as engineered DNA-binding molecule-mediated...

3. QC Sequencing Technologies at Addgene

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   Blog Post

   ...synthesis-based sequencing methods (Figure 3). It uses (aptly named) “nanopores” that are attached to the flow cell ...

4. Plasmids 101: Shuttle Vectors

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   Blog Post

   ...aminoglycosides work on different host species: kanamycin targets the prokaryotic ribosome, while G418 (...

5. Antibodies 101: The Four ELISAs and When to Use Them

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   Blog Post

   ...method are similar to those of the direct method. Namely, since the assay requires a single conjugated primary...

6. How to Be an Excellent Trainee

   Type

   Blog Post

   ...can do a lot to contribute to the relationship dynamic and set yourself up for success! Getting the most...

7. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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   Blog Post

   ...hypothetical protein, improving expression, and named this gene set bARGSer, for bacterial ARG from Serratia...

8. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   Blog Post

   ... Table 1: Comparison on Common CRISPR Enzymes Name  Enzymatic Domains Guide RNA Target PAM (PFS) Cleavage...

9. To Codon Optimize or Not: That is the Question

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   Blog Post

   ...2006) and mRNA stability (Hausser et al., 2013) to name a few. This could affect synonymous mutations which...

10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post

    ... of near-PAMless Cas9 variants. These variants, named SpG and SpRY, can target NGN PAMs and NRN and NYN...

11. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post

    ...relying solely on GFP (Katti et al., 2020). They named this reporter “Gene On” or GO. GO works by affecting...

12. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post

    ...modifications to improve their detection system. They named this system SHERLOCKv2 for SHERLOCK version 2. Here...

13. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    Blog Post

    ... networks The last stage is Exponential Growth, named after the growth phase when bacteria in favorable...

14. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ... DNA yielded a new CRISPR effector protein they named Cas7-11. Testing showed that Cas7-11 cut RNA with...

15. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    Blog Post

    ...and how does it work? FLP recombinase, which is named after its ability to invert or ‘flip DNA’, is derived...

16. Supporting Reproducibility with a Connected ELN

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    Blog Post

    ...static and isolated vehicle and becomes part of the dynamic and connected record of research. In RSpace, document...

17. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    Blog Post

    ...researchers to investigate the localization and dynamics of fluorescent protein fusions in live cells in...

18. Important Considerations in Optogenetics Behavioral Experiments

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    Blog Post

    ...methods 9.2 (2012): 159-172.5. Goshen I, et al. Dynamics of retrieval strategies for remote memories. Cell...

19. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post

    ... the human ubiquitin C gene Constitutive  As the name implies, this promoter is ubiquitous. human beta...

20. CRISPR Guide

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    Guide

    ...as Cas9 or Cas12a. These are identified in their names — AcrII proteins inhibit Cas9 (CRISPR Type II) systems...Bxb1 installs the donor sequence. Bxb1 versions named evoBxb1 or eeBxb1 are evolved versions of the recombinase...CRISPR imaging can visualize chromatin and RNA dynamics in live cells. Labeling can be done by fusing ... Fluorescent CRISPR systems have been used for dynamic tracking of repetitive and non-repetitive genomic...Cse1), Cas11 (Cse2), Cas7, Cas5 and Cas6e. As the name implies, this system is harnessed for antiviral ...Weissman, J. S., Qi, L. S., & Huang, B. (2013). Dynamic imaging of genomic loci in living human cells by...