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Showing: 341 - 360 of 758 results

  1. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...symposium, a panel of thought leaders in the field--including Shinya Yamanaka, George Q. Daley, Christine Mummery... promising clinical trials for ALS.   I have an uncle with Parkinson's, a half-brother who is a quadriplegic...

  2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...that can influence the HDR/NHEJ decision. Our conclusions reinforce a key scientific principle: that understanding...frequencies of genome cutting and, when donor DNA is included in the nucleofection reaction, extremely high ...

  4. Advice for Scientists Starting a Lab

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    Blog Post
    ... Tom Ellis was offered a start-up package that included a postdoc and a PhD student in his first year.... great resources for Early Career Scientists, including their "Making the Right Moves: A Practical Guide...

  5. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is...in the cell, as many of these experiments are concluded within 48 h of transfection. Of course, it is ...

  6. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...backbone to generate R-mAbs of different subclasses, including subclasses that were not produced by the original...expanded their recombinant affinity reagent tools to include nanobodies. At Addgene, we are excited to be part...

  7. Antibodies 101: Isotypes

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    Blog Post
    ...controls are used in antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry...region length. Now for some essential isotype nomenclature. Antibody heavy chain proteins as well as the...

  8. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...generate antibodies against many types of molecules including proteins, lipids, and carbohydrates, in this blog...2005). This is reflected in the antibody’s cost. Conclusion Whether you choose to use a monoclonal antibody...

  9. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...shares many common features with gamma-retrovirus including genome architecture, host integration, and its...its cargo, in fact its viral life cycle does not include genomic integration. While the expression of adenovirus...

  10. Viral Vectors 101: Viral Applications

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    Blog Post
    ... tools with many applications. These libraries include sgRNAs targeting the entire genome, overexpression...several advantages over traditional vaccines, including 1) cellular responses in addition to antibody ...

  12. Behind the scenes: Addgene’s new search engine and more

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    Blog Post
    ...80,000+ reagents and 700+ educational resources — including blog posts, protocols, guides, and collections...we rolled out multiple changes to our website, including the recent homepage and search tool updates. Now...

  13. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post
    ...The information is organized in wiki format and includes biochemical properties, previously established...to browse entries by the current phylogenetic nomenclature and discover orthologs with similar properties...

  14. Plasmids 101: Inducible Promoters

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    Blog Post
    ...Other examples of chemically induced promoters include positive inducible alcohol and steroid regulated... be induced by heat or cold exposure. Examples include the heat shock-inducible Hsp70 or Hsp90-derived...

  15. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...sociology, behavior, and artificial intelligence (including a team that created a novel form of AI that can...require large data sets. Whether these data sets include videos of behavior, many many images of brain regions...

  17. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...nano-lanterns for use in various applications, including optogenetics, biosensors, and fusion proteins....al. expanded the Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these...

  19. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...expression of other endonucleases, like Csy4, or the inclusion of processing signals, such at tRNAs, to process...its own pre-crRNA arrays eliminates the need to include multiple promoters to drive crRNA expression and...

  20. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ... DNA there, although other restriction sites (including other XbaI sites not preceded by GA or followed...either be difficult to interpret or lead you to conclude your plasmid is not correct! Conversely, enzymes...
Showing: 341 - 360 of 758 results