We narrowed to 457 results for: nsf

1. RaPID Detection of RNA-protein Interactions

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   Blog Post

   ... include both scrambled RNA motifs and cells transfected with BirA* protein alone (i.e. no RNA bait). ...

2. Human Germline Editing Using CRISPR

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   ...off-target effects, but this advantage likely won’t transfer to all genes we’d like to target. In addition,...

3. What's New in CRISPR - November 2021

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   ...They further develop a version of SEND for gene transfer with pseudotyped viral particles, expanding options...

4. Giving gRNAs a Facelift - Synthetic and Beyond

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   ...targeting experiments allows you to visualize transfection efficiency and even track localization in cellular...

5. Antibodies 101: Affinity Reagents

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   ...bonds, monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used...

6. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   ...on the Addgene blog: Check out this guide on Transferable Skills: External Collaborations Learn about ...

7. Celebrating 15 Years of Scientific Sharing

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   Blog Post

   ...lauded by another set of stakeholders - the tech transfer community. In a recent law review article we received...

8. Developing Lab Management Software for Biology

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   ...t have a system through which she could easily transfer her instructions for dealing with the samples ...

9. Addgene's Tips for Plasmid Quality Control

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   Blog Post

   ...production and storage, we grow a little extra and transfer 100 µl of glycerol stock to each of 2 96-well ...

10. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    ... in their 293 cell culture system. In cells cotransfected with GFP and the GBPs, GFP linked the two GBPs...

11. The Future of Research Symposium Boston 2015

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    ...PMCID: PMC3342243. 4. Mathur, Ambika, et al. "Transforming training to reflect the workforce." Science ...

12. Making CRISPR Plasmids Using Fragmid

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    ...Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system...

13. CRISPR in the Clinic

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    ...CRISPR targets — Sickle Cell Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which...

14. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...mixing with the liver paste, these E. coli are transformed with the appropriate plasmid and dsRNA expression...

15. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...Stoica L, Ahmed SS, Gao G, Sena-Esteves M. Gene transfer to the CNS using recombinant adeno-associated ...

16. Hot Plasmids - October 2020

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    ...lentiviral CRISPR library that targets human glycosyltransferase genes and other proteins related to cellular...

17. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging chromatin structure...

18. How to Prepare for an Industry Interview

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    Blog Post

    ... an opportunity to demonstrate technical and transferable skills that make you qualified for the position...

19. Hot Plasmids and Viral Preps - March 2021

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    Blog Post

    ...cell surface. When the cells interact, GFP is transferred to the receiver cell, and the receiver cell turns...

20. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    Blog Post

    ...their mutations corrected, and the T-cells were transferred back into the infants. Almost all patients have...