...6).   Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing...

...Gluconacetobacter xylinus, Gluconobacter spp., Hyphomicrobium spp., Klebsiella aerogenes, K. pneumoniae, ...

... promoter. For example, neurons treated with a phorbol ester activator gave fluorescent signal in the ...

...but rather because of the correct folding and fluorophore formation by most, if not all, of the FP fusions...

...Probably the easiest way to do this is to leave your phone in your bag on “Do Not Disturb” during the times...

...440 or 1000bp overlaps to deliver the alkaline phosphatase gene to airway epithelial cells in mice. This...

...cleaves a nearby RNA sequence that contains a fluorophore that is quenched when intact and released when...

... my applications all by snail mail and only had phone interviews. We didn't have Skype in 2004. I also...

...supposed to be done. They will start looking at their phones. Speaking longer just makes you seem unprepared...

...plasmids for the mutation of interest. Gel electrophoresis diagrams of the anticipated restriction analysis...

...Khalili K. Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing. Sci Rep. 2016...

...possible, this even includes clocks, timers, and phones.  As for reagents and consumables (such as pipet...

...help scientists problem solve experiments over the phone or explain how various plasmids work. Most people...

...Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment...

...edit. Using Surveyor nuclease followed by gel electrophoresis, these mismatches are indicated by the presence...

... The results are then visualized using gel electrophoresis. Surveyor™ and T7E1 have been widely adopted...

.... Many plasmid elements such as promoters or fluorophores are interchangeable across organisms. When using...

...431098 Sodium phosphate monobasic monohydrate, Millipore Sigma 71507-250G Sodium phosphate dibasic, Millipore...sterilize 1 M of sodium phosphate buffer, pH 7.0 390 mL of sterile sodium phosphate monobasic monohydrate...binding buffer (20 mM sodium phosphate buffer, pH 7.0) 20 mL 1M sodium phosphate buffer 980 mL of deionized...compatible with 50 mL conical tubes NanoDrop spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform...RT) before use. Reagent Preparation 1 M sodium phosphate monobasic monohydrate (NaH 2 PO 4 ∙H 2 O), pH ... 7.0 Autoclave or filter sterilize 1 M sodium phosphate dibasic (NaH 2 PO 4 ), pH 7.0 142 g of NaH 2 PO... (NaH 2 PO 4 ∙H 2 O) 610 mL of sterile sodium phosphate dibasic (NaH 2 PO 4 ) Store up to 1 month at 4...

...with a phosphatase before performing the ligation reaction (phosphatase removes the 5' phosphate and therefore...was completely digested and if phosphatase treated, that the phosphatase treatment worked. This control... ligase catalyzes the formation of covalent phosphodiester linkages, which permanently join the nucleotides... vector re-circularization - most useful for phosphatase treated vector Insert or water + Any colonies...

...purity. This can be done with a spectrophotometer. A spectrophotometer uses the absorbance/transmission... instructions for the spectrophotometer specific to your lab. Spectrophotometer Tips Before measuring ...labs have a NanoDrop, which is a very small spectrophotometer that can accurately read DNA concentration...measuring any samples, be sure to ‘blank’ the spectrophotometer using the solution the DNA is resuspended in, ...NanoDrop. It is a good idea to re-zero any spectrophotometer between samples if they are expected to vary...