We narrowed to 498 results for: plasmid dna

... about FLEx vectors Download our Plasmids 101 eBook Read our plasmids blog posts Resources on the Addgene...molecular biology reference Find Cre-lox plasmids at Addgene Browse plasmids at Addgene ...site-specific recombinases such as Cre or Flp that induce DNA recombination at defined recognition sites (i.e.,...Wolf, and R. J. Samulski. "Delivering transgenic DNA exceeding the carrying capacity of AAV vectors." ...

...Kotton Learn How Plasmid Sharing Speeds Science Resources at Addgene.org Order Plasmids from Addgene! ...to engineer microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast...community to collect various soil samples, extract DNA, sequence it, and analyze the data to characterize...

... viability controls such as DNA binding dyes and amine reactive dyes. DNA binding dyes, such as propidium...membranes as is the case with dead or dying cells. DNA binding dyes are not compatible with protocols that...amine reactive dye, such as LIVE/DEAD stain. Like DNA binding dyes, amine reactive dyes can only pass through...Visit the Fluorescent Protein Guide Find antibody plasmids for your experiments ...

...for verifying plasmids like this is "plasmid fingerprinting", where you cut the plasmid into 3-8 pieces...Digest You may also like... Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis Introduction Restriction... to selectively move a specific piece of DNA from one plasmid to another. A diagnostic restriction enzyme...enzymes cleave DNA at specific sequences called restrictions sites. Often, the size of the plasmid insert and...used to verify your plasmid. The goal of a diagnostic digest is to cut your plasmid into specific sized...restriction enzyme or enzymes that cut your plasmid. Many DNA analysis tools, including Addgene’s Sequence... Pipetman Pipet tips Reagents Liquid DNA aliquot of your plasmid of interest (see below for recommended...

...in a 1-plasmid system, in which Cas9 is included on the gRNA-containing plasmid, or a 2-plasmid system...contain thousands of gRNA plasmids, discerned only by a unique barcode on each plasmid. As such, sequencing...gRNA generates a double-strand break in the target DNA, which, when repaired through error-prone non-homologous...are composed of many different gRNA-containing plasmids, each with a different genomic target. The goal...sequences and ease of generating gRNA-containing plasmids. CRISPR genome wide-screening experiments commonly...other types of experiments, check out our CRISPR Plasmids and Resources Page. References McDade JR, Waxmonsky...Lentivirus Get Tips on Titering Your Lentivirus Plasmids 101: NGS Quality Control for Pooled Libraries ...

...subfields.  Plasmid requests correlate with paper publication The dataset includes 11,426 plasmid requests...paper has between 1–32 plasmids and a total of 2–2,590 requests. We received plasmid requests from 56 countries...Spikes in plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests...number of plasmids ordered for each paper. Rough estimation of COVID-19 effect on plasmid requests ...majority of our plasmid orders come from the U.S. and Europe, we see a corresponding dip in plasmid request activity...of how plasmid orders were affected by the COVID-19 pandemic, we compared the number of plasmid orders...Learn more about plasmid sharing during COVID-19 Resources on Addgene.org Find plasmids from David Liu's...

...transcription factors – to specific sequences of DNA has become commonplace. This ability has opened up...and improve, Abil says they will make those new plasmids available through Addgene, and they hope others...

...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic... genes from specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced...interactions between DNA and protein. Micrococcal nuclease digestion is used to shear the DNA in these assays... uses antibodies to capture protein:DNA complexes. After capture, the DNA is released from the complex...suitable for DNA:histone modifier interactions, which have strong binding between the protein and DNA. Some ...immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals make...genetic code inserted into the plasmid. This produces the least amount of variation between antibodies, allows...

...Addgene.org Find plasmids from the Bethune Lab Find Plasmids from the Roux Lab Find Plasmids for FRET ...Learn about plasmids for in vivo biotinylation of bacterial fusions proteins Study Protein-DNA Interactions...BirA* fusion proteins are expressed in cells via plasmids or by the creation of stable cell lines and these...Interested in using Split-BioID with your research? Plasmids are available from Addgene! References Schopp... Central PMCID: 5467174. Find the Split-BioID Plasmids Here! Munter, S.D., Görnemann, J., Derua, R.,...site than in Schopp et al. Find these Split-BioID Plasmids Here!   Burke, B., Kim, D.I., Roux, K.J., & Raida...PubMed Central PMCID: 3308701. Find the BioID Plasmids Here! Macneill, S., & Varnaitė, R. (2016). Meet...

...Promega Plasmid Collection Promega Plasmid Collection Ordering & Availability All Plasmids Plasmid Technology...agar stab 20 µg purified DNA in Tris buffer (500 ng/µL ± 25 ng/µL) All plasmids in the collection are available...our help center . Plasmid Collection Browse and sort the table below to find plasmids in the collection...a new window) on Promega.com. ID Plasmid Description Industry Plasmid Technology Overview This collection...Browse Addgene's Luciferase Plasmid Collection Explore other Promega plasmids available at Addgene Find ...operational complexity. This unique collection contains plasmids developed by Promega scientists and designed to...HaloTag, SmBiT, and LgBiT. Ordering & Availability Plasmids from the Promega collection are available in two...

...Recovering Plasmid DNA from Bacterial Culture Restriction Digest of Plasmid DNA Background Information During...your DNA sample concentration and purity using Addgene’s DNA Quantification Protocol. Protocols...spectrophotometer using the solution the DNA is resuspended in, but with no DNA added. 'Blanking' measures the ... Protocols DNA Quantification...Quantification DNA Quantification You may also like... Inoculating a Liquid Bacterial Culture Recovering...important to get a quick and accurate reading of DNA concentration and purity. This can be done with a... maximally absorbs. In the case of nucleic acid (DNA and RNA), the maximal absorbance is at 260nm. Protein...

...stocks of their plasmids. This way, when you want to make more plasmid DNA, the plasmid will already be... for long-term storage of plasmids. Although you can store your plasmid DNA at -20°C, many labs also create... Storage of Plasmids Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids You may also... like... Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Background Information...able to start an overnight culture for plasmid DNA prep the following day. Tips and FAQ The optimal concentration...glycerol stock the same time you prepare your plasmid DNA. In the morning, when you retrieve your liquid... and growth temperature, can be found on your plasmid's information page. The next day you will be able...

...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles...barcoding Addgene’s own AAV6 and AAV11 packaging plasmids Mapping anterograde synaptic connections with ...experiments and see the difference! Find mGold2 plasmids here! Lee, J., Lai, S., et al. (2025). Bright...Effector nucleases are shown bound to the target DNA, recruited by either the ωRNA (TnpB) or a gRNA (Cas9... to the broad host range of TRV. Find TRV-TnpB plasmids here! Weiss, T., et al. (2025). Viral delivery...j.stem.2025.01.012.  New AAV6 and AAV11 packaging plasmids from Addgene By Meghan Rego Attention AAV users...delivery toolkit, Addgene now offers packaging plasmids for AAV6 and AAV11! Why these serotypes? AAV6 ...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...Hutchinson Cancer Research Center developed a pooled plasmid library encompassing all possible amino acid mutations...anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes the mutated spike protein and a barcode...head over to our CRISPR Plasmids and Resources page.  A new system uses light to deactivate photocleavable... accumulation of indels to measure time. These plasmids with different promoters driving expression of...

...consisted of three plasmids: the packaging, envelope, and transfer plasmids. The packaging plasmid carried a mutated... packaging and envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles with...Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: ...itself due to a few missing proteins. The envelope plasmid contained a viral envelope to dictate the tropism...the vector could infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 ...genome integration. After co-transfection of these plasmids, 293T cells released transgene-containing lentiviral...recombination between the transfer and packaging plasmids does exist. To further reduce this possibility...

...-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids ...uses non-homologous end joining (NHEJ) to perform DNA double strand break repair instead of homologous ...the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the...

... Some researchers have used fluorescently tagged DNA-binding proteins to label certain loci, but this ...24360272. PubMed Central PMCID: PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui...25713381. PubMed Central PMCID: PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui...27088723. PubMed Central PMCID: PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira...26344044. PubMed Central PMCID: PMC4666719. Find plasmids from this publication at Addgene. Additional ...Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones...

...interaction due to mismatches between the guide and the DNA target. CFD scores are then modified (“transformed...sequence, one by one, from the PAM outwards so that the DNA sequence is altered but the protein sequence they...software Resources on Addgene.org Find CRISPR plasmids and resources Download the CRISPR eBook Read our...

...reconstituted after integration; and a sequence of stuffer DNA after the gRNA target site. Donor B contains: 400...Since this approach is dependent on double-strand DNA break repair, if non-homologous end joining (NHEJ...2013) Oversized AAV Transductifon Is Mediated via a DNA-PKcs-independent, Rad51C-dependent Repair Pathway...therapy Learn about the parts of an AAV transfer plasmid Resources on Addgene.org: Check out the Addgene...

...You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation Background ...stranded DNA template strands. The DNA polymerase is also stable enough to now bind to the primer DNA sequence...which would denature DNA polymerases from other species. In addition to the template DNA and the Taq polymerase...several micrograms of target DNA from just a few nanograms of template DNA through several cycles of denaturation...this step enables the DNA polymerase to synthesize and elongate the new target DNA strand accurately and... are using the same template DNA for all your reactions, the Template DNA can be added to the master mix...the number of nanomoles of DNA times 10. For example, if your lyophilized DNA is 38.5nm, add 385µl of water...