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...clone 500-700 bp homology arms for your gene of interest into the FP-SEC vector using Gibson assembly. ...as well as a promoter-FP fusion for the gene of interest. L1/L2 larvae from these strains are then heat...applications, SEC selection could replace other, more restrictive C. elegans selectable marker systems. This novel...

...identified some fly stocks and other reagents of interest, the next question to ask is what to do with them...fly line expressing Gal4 in your tissue type of interest. You can quickly mate your UAS-YFG line with a...different tissue types! Although putting a gene of interest directly downstream of a tissue-specific promoter...

...all viral genes are flanked by EcoRI and BamHI restriction sites - so cloning projects should be fun and...researchers. We have been excited to see so much interest, because it means more scientists will be working...Communications Team at QBI UCSF. They are particularly interested in facilitating collaborative science, and making...

...determine if the fluorescent proteins you are interested in are compatible with your microscope optics...related protein, if you know that your protein of interest has a similar spectrum. For example, here's a ...need to try several proteins in your system of interest. Finally, in this set of proteins the green and...

...first developed in the 1990s. While traditional restriction enzyme cloning uses short sticky ends to insert.... To start the SLIC cloning process, a gene of interest is PCR amplified to add 5′ and 3′ homology regions...multiple fragments without the need for specific restriction sites or DNA ligase! This post was originally...

...efficiency. However, gamma-retrovirus infection is restricted to dividing cells only, so is not suitable for...can target the virus to specific cell types of interest. This is a particularly nice feature as organ-...have an idea which ones could be helpful to you. Interested in learning more about a specific virus? We have...

...tumorigenesis The conference opened with a very interesting presentation by Professor Guido Kroemer from ...presentation, starvation or the use of caloric restriction mimetics trigger autophagy and, in a mouse model...References: 1. Pietrocola, Federico, et al. "Caloric restriction mimetics enhance anticancer immunosurveillance...

... indels for “functional knockout” of a gene of interest is one of the most common applications of precision...important difference (Figure 3). If you are interested in harnessing the power of digital PCR screening...Postdoctoral Fellow at the University of Alberta. He is interested in everything molecular biology. Follow him on...

...the bacteria can easily uptake your plasmid of interest. Scientists can also use electroporation, the ...scientists to introduce different DNA sequences of interest into a bacterial cell or a host’s genome. To do...create both transient cell lines, where a gene of interest is expressed but not integrated into the genome...

...coli, carrying a plasmid expressing a protein of interest). Mesophilic microbes are highly preferable (and...delighted to engage with potential collaborators and interested audiences. Please visit our team wiki or contact...at the iGEM Competition 2019. He has an active interest in scientific writing and science communication...

...activated B cell that produces the antibody of interest with a myeloma cell. This RNA was then used to...Constructs were then verified by both diagnostic restriction digest and colony PCR.   Fig. 1: (A) ... found that the recognition sequence for the restriction enzyme BciVI was present in the aberrant light...

...been hampered by the need to tag each protein of interest and, for in vivo work, generate a dedicated transgenic...GFP-nanobody rather than a specific protein of interest. This fusion protein can then be co-expressed ...expressing a GFP-tagged version of a protein of interest. The binding of the GFP nanobody to its target...

...Researchers express genes of interest from plasmids in order to study gene function or to engineer cells...fluctuations due to copy number by introducing genes of interest into the chromosome. But this has its own set ...thereby intensifying repression of the gene of interest and keeping its expression level constant. In ...

...Health Science Center at Houston. Prakash became interested in studying TS when he was studying thoracic ... Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in TS when I was a newly independent faculty ...channels in the brain.  “I have a long standing interest in the biology of GPCRs and G proteins, with an...

...doing after the injunction? We are particularly interested in whether institutions planning to raise salaries...our FOIA requests, we are starting to see very interesting data on how much postdocs are actually getting...Executive Director of Future of Research. He is interested in advocating for and with junior scientists,...

...fail. Our laboratory manager would definitely be interested to see which part of her procedures are the most...getting up and running and more time working on interesting biotechnology code.There are valid technical ...Shamloo is a Software Engineer at Addgene and is interested in writing software tools to help scientists....

...them a quick scan for interesting key words and download what seems interesting or relevant. Harness the...keeping up with the literature in your field of interest. Set aside some time each day or week to at least...

...which may be of particular interest to MoClo users!). If you’re interested in complex Golden Gate Assembly...overhang set by uploading the set with the Type IIS restriction enzyme and thermocycler protocol. It will also...

...When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with biotin. ...difficult to detect proteins with short half-lives. Interested in using Split-BioID with your research? Plasmids...

...in plants to conditionally deplete proteins of interest in C. elegans (Zhang et al., 2015). In this system...spatially and temporally knock-down proteins of interest.  Plasmids for cGAL and split cGAL systems The...halves of cGAL can drive expression of your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene...