...figures of interest along with the article metadata, legend and user created notes on a “Pinterest for scientists... scientific literature? Very often the data of interest are in 1 or 2 figures from one paper and then ...

... Short segment of DNA which contains several restriction sites allowing for the easy insertion of DNA....“vectors” or “constructs.” To insert a gene of interest into a vector, scientists may utilize one of a... a variety of cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning...

...NEBCutter2: For finding restriction enzyme cut sites Webcutter 2.0: For finding restriction enzyme cut sites ... different types of cloning methods, such as restriction enzyme cloning, Gibson assembly, or In-Fusion...

...plasmid that produces biotin ligase and a protein of interest fused to maltose binding protein (MBP, New England...-Avi-His/BirA to add biotin to your protein of interest Grow fusion protein plasmid in NEB T7 SHuffle... Vanderbilt Eye Institute and is particularly interested in cell signaling.     Additional Resources on...

...several excellent guest posts from our depositors. Interested in writing your own post for the Addgene Blog...(AAVs) are commonly used to deliver a genes of interest to a variety of cell types in-vivo. These are ...customizable functions to your DNA sequence of interest. Potential applications of the system include ...

...collection, you'll likely find some proteins of interest for which you have several antibodies in stock...another, or if you’re looking for a protein of interest while it’s in a transition state or specific configuration... to validate that it recognizes the protein of interest in specific configurations or conditions. In those...

...easiest and clearest ways to determine if your sample has recombined. Choose a restriction enzyme that...one resulting plasmid carries the transgene of interest (aka the viral genome) and the other contains ...

...overcome. Conflict, and its inherent tension, is interesting. It’s what drives a story forward - and this ... the pieces that make you think, “Oh, that’s interesting! How did you solve that problem?” Those are the...your audience in with something that sparks their interest and makes them want to know more. This could be...

...2008 the Quake Lab at Stanford University became interested in exploring biological dark matter – large tracts...like YNP thermal sites - are also of practical interest since they’ve previously yielded valuable enzymes...bacterial RNA-Seq. This enriches for transcripts of interest without an increase in the number of steps in ...

...clone 500-700 bp homology arms for your gene of interest into the FP-SEC vector using Gibson assembly. ...as well as a promoter-FP fusion for the gene of interest. L1/L2 larvae from these strains are then heat...applications, SEC selection could replace other, more restrictive C. elegans selectable marker systems. This novel...

...all viral genes are flanked by EcoRI and BamHI restriction sites - so cloning projects should be fun and...researchers. We have been excited to see so much interest, because it means more scientists will be working...Communications Team at QBI UCSF. They are particularly interested in facilitating collaborative science, and making...

...identified some fly stocks and other reagents of interest, the next question to ask is what to do with them...fly line expressing Gal4 in your tissue type of interest. You can quickly mate your UAS-YFG line with a...different tissue types! Although putting a gene of interest directly downstream of a tissue-specific promoter...

...determine if the fluorescent proteins you are interested in are compatible with your microscope optics...related protein, if you know that your protein of interest has a similar spectrum. For example, here's a ...need to try several proteins in your system of interest. Finally, in this set of proteins the green and...

...the bacteria can easily uptake your plasmid of interest. Scientists can also use electroporation, the ...scientists to introduce different DNA sequences of interest into a bacterial cell or a host’s genome. To do...create both transient cell lines, where a gene of interest is expressed but not integrated into the genome...

...tumorigenesis The conference opened with a very interesting presentation by Professor Guido Kroemer from ...presentation, starvation or the use of caloric restriction mimetics trigger autophagy and, in a mouse model...References: 1. Pietrocola, Federico, et al. "Caloric restriction mimetics enhance anticancer immunosurveillance...

... indels for “functional knockout” of a gene of interest is one of the most common applications of precision...important difference (Figure 3). If you are interested in harnessing the power of digital PCR screening...Postdoctoral Fellow at the University of Alberta. He is interested in everything molecular biology. Follow him on...

...first developed in the 1990s. While traditional restriction enzyme cloning uses short sticky ends to insert.... To start the SLIC cloning process, a gene of interest is PCR amplified to add 5′ and 3′ homology regions...multiple fragments without the need for specific restriction sites or DNA ligase! This post was originally...

...been hampered by the need to tag each protein of interest and, for in vivo work, generate a dedicated transgenic...GFP-nanobody rather than a specific protein of interest. This fusion protein can then be co-expressed ...expressing a GFP-tagged version of a protein of interest. The binding of the GFP nanobody to its target...

...activated B cell that produces the antibody of interest with a myeloma cell. This RNA was then used to...Constructs were then verified by both diagnostic restriction digest and colony PCR.   Fig. 1: (A) ... found that the recognition sequence for the restriction enzyme BciVI was present in the aberrant light...

...efficiency. However, gamma-retrovirus infection is restricted to dividing cells only, so is not suitable for...can target the virus to specific cell types of interest. This is a particularly nice feature as organ-...have an idea which ones could be helpful to you. Interested in learning more about a specific virus? We have...