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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 538 results for: sequence

Showing: 341 - 360 of 538 results

  2. Tetracycline Inducible Expression

    Type
    Collection
    ...protein (TetR) and operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions, the...called Ptet) have been designed by combining tet O sequences with elements like the H1 RNA promoter. And, most...or other species by codon optimization of their sequence and eliminating interactions with endogenous factors...Bujard, H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent transcriptional...

  3. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...positive or negative selection). Next-generation sequencing (NGS) to identify genes of interest. ...Kampmann, M. (2023). CRISPR screening by AAV episome-sequencing (CrAAVe-seq) is a highly scalable cell type-specificin...

  5. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...circuits. Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that...of proteins that are highly similar in terms of sequence and thus very difficult to distinguish using standard...

  6. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...cytometric antibodies, downstream of the TUJ1 coding sequence. ES cells that stably express the dCas9-Suntag...cells were then isolated and their genomic DNA sequenced. Guide RNAs targeting several known and some unknown...

  7. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional and lab...to the associated data (spreadsheets, images, sequencing data, etc.) used and/or referenced in the experiment...

  9. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...Droplets that fluoresce contain the amplified target sequence. After all of the droplets have been read, the...reason, it can be used in the detection of rare sequences and single cell analysis. It is also being used...

  10. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ... still able to bind to the upstream activation sequence (UAS) of its target gene, in this case a reporter...downstream of a promoter containing the target sequence of the DBD. The DBD-POI fusion (commonly referred...

  14. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...than BE3, but APOBEC3G displayed less predictable sequence preferences.  The Liu lab also used natural and...develop five new base editors with distinct PAM sequences, expanding the number of available target sites...evolution efforts yielded an editor with more flexible sequence preferences, evoAPOBEC1-BE4max, which is significantly...editing, has allowed scientists to replace entire sequences of varying lengths. Base editing publication ...

  15. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...testing, and sterility testing, plus viral genomic sequencing to confirm the identity of the packaged DNA and...

  16. Editor's Choice, October 2016

    Type
    Blog Post
    ...science and more! All Posts from October 2016: Sequencing Options for CRISPR Genotyping Grad School Advice...

  18. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...the University of California, Santa Cruz, offers sequence data on five strains of Ebola representing the...

  19. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...generates a double-strand break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are...
Showing: 341 - 360 of 538 results