We narrowed to 526 results for: sequence

...plasmid encoding the insert of interest as well as sequences necessary to encapsulate that insert into a viral...plasmid and the packaging plasmid caused the Psi sequence to be placed on the packaging plasmid forming ...composed of the wild-type 5’ and 3’ LTRs, the ψ sequence, a part of the env gene containing the rev response...

...[2]. Delivering dsRNA matching a specific mRNA sequence triggers a cascade of events that results in the...genes not in this collection, they may clone DNA sequences for creating riboprobes and dsRNAs into PR-T4P...

...fortunate to hand-deliver the model, the following sequence of events plays itself out: The researcher breaks...along the double-stranded DNA, recognize a PAM sequence, allow the complementary base pairing between ...

...by gel electrophoresis and/or next-generation sequencing more appropriate? If you already have an end...and sgRNA efficacy by cloning in genome target sequence into EGFP reporter. Target DNA reporter system...

... to ship plasmids or viral vectors containing sequences from rabies and all other lyssa genus members ...

... string of amino acids that preserves the sequence of your gene and of the fusion protein. Read more...backbone for cloning and expression of new shRNA sequences Zeocin®/Bleo Mammalian lenti sgRNA(MS2)_zeo backbone...

...iteratively generated 3D models and amino acid sequences that got closer and closer to achieving it. Once...

... blog post, the team developed a viral genome sequencing strategy to confirm the identity and purity of...

...the PCR product should contain the edited BRAF sequence), and PCR-amplify the BRAF locus from control ... PCR product should contain the wild-type BRAF sequence). Then, mix the PCR products and use temperature... was challenging because you’re limited by the sequence of the human genome. I used the UCSC genome browser...

...more closely about the wealth of high-throughput sequencing (HTS) data we generate and maintain. It became...begin! Figure 1: Discussing next-generation sequencing at the Addgene bioinformatics journal club! ...

...plasmid. Of course, other follow up methods like sequencing are more definitive, but visual identification...optimization programs to engineer 14 chromoprotein sequences into E. coli BioBrick plasmids (meffRed, eforRed...

...itself and the degron off at the protease cleavage sequence. The tag is then shuttled to the proteasome due...the protein around, leaving a minimal additional sequence (7 kDa). This system also requires less genetic...

...EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these sequences are not copied...

...ensuring the cells used are meant for repetitive sequences (such as STBL4 or Endura Duo), growing your bacteria... pCMV-Rep 78/68 SSM Library Amplification and Sequencing Protocol (DOCX, 32 KB) Screening Rinehart...

... engineered genetic sequences; but it’s only the promises and associated sequence information. Addgene...

...worm ( C. elegans, C. briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene...

...fluorescent signal. As for linker sequences, the amino acid sequences RSIAT and RPACKIPNDLKQKVMNH have ...

...Droplets that fluoresce contain the amplified target sequence. After all of the droplets have been read, the...reason, it can be used in the detection of rare sequences and single cell analysis. It is also being used...

...positive or negative selection). Next-generation sequencing (NGS) to identify genes of interest. ...Kampmann, M. (2023). CRISPR screening by AAV episome-sequencing (CrAAVe-seq) is a highly scalable cell type-specificin...

...cytometric antibodies, downstream of the TUJ1 coding sequence. ES cells that stably express the dCas9-Suntag...cells were then isolated and their genomic DNA sequenced. Guide RNAs targeting several known and some unknown...