... quick zap of the proper color light, you can do just that.  Photosensitizing dyes and proteins have been...injecting adult fish with the dye. Embryos exposed to just 12 minutes of illumination had fewer mCer3-labeled...KillerRed reaching greater than 75% photobleaching after just 1 minute of exposure. MG-2I-dL5** experienced minor...

...in-depth research is applicable to a wide range of industries and is a highly desirable quality sought after...skills include: Science Reporter Scientific Illustrator Research and Development Scientific Advisor..., including: Livestock Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist...

... cells.) Using this system, virus is produced in just a few days. Another variant, Phoenix-gp, contains... from an internal promoter. An internal promoter must be included as the wildtype LTR promoter is no longer...started by 5' LTR by the addition of a poly A tract just after the R sequence. This post was written ...

...pseudotyped to alter infectivity. Next, the virus must assemble and have appropriate factors displayed ...Adenoviruses have quite a bit in common with AAV, just like lenti and gamma-retroviruses have a lot in ...is a linear, dsDNA virus naturally. It has ITRs, just like AAV, which encompass the expression construct...

...in your gene of interest, for example. Some customization is required, but many viral constructs have ...elements simultaneously in your system instead of just one at a time. Many of these libraries are packaged...of each variable being manipulated. Thus, the adjustable nature of viral libraries makes them the ideal...

... in August 2017.  This flexible teaching space offers STEM programming, workshops, and customized programs...resulted in a new BioBuilder book chapter and an illustrated, downloadable classroom poster — with both of...

...recombination. Heavy chain constant genes are clustered together downstream of the variable region gene...they are the first constant domain genes in the cluster. However, when B cells switch to expressing a different... genes in the heavy-chain constant domain gene cluster. DNA recombination can delete intervening heavy-chain...

...cannot offer. Furthermore, companies across most industries tend to hire their former interns to fill entry-level...Consequently it’s best to draft a cover letter that is customized for each internship. Lastly, have your resume...interview prep Prepare for your virtual interview just as you would for an in-person interview. Formulate...

...,850 SARS-CoV-2 plasmids to help academic and industrial labs accelerate research and to help find a faster...research more visibility.    Figure 1: Illustration of SARS-CoV-2 from Philippa Steinberg for the...March. The first set of 383 constructs went online just three weeks following initial receipt on site.  ...

...interpret and analyze at a glance. For a quick look at just how much things have improved, check out the example...sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this can be found on the...BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using...

...affected by methylation. This table is by no means exhaustive, so you may want to consult the REBASE database...site blocked by methylation), the figure below illustrates how sequence visualization software (we used ...altering your choice of bacteria. For example, if you must use a restriction site that will be blocked by Dam...

...insertions, it’s possible to use the same technique just using a large primer known as a megaprimer. Piecing...144 kb from a bacterial artificial chromosome in just one reaction. Using other methods, such “BAC shaving...potential pitfall of REPLACR-mutagenesis is that you must PCR amplify the whole plasmid, which could introduce...

...yeast cells. To be useful in the lab, the vectors must contain a yeast-specific origin of replication (...Integrating plasmids (YIp): These plasmids lack an ORI and must be integrated directly into the host chromosome ...known auxotrophic strain/ selection element pairs must be utilized or a new combination needs to be created...

... 3 hour breakfast once a month. All participants must commit to making the meetings a scheduling priority...meeting.  Scientists do better with process, so don’t just get together and chat. Have an agenda and get everyone...potluck dinner. Invite guests from a lab, career or industry of interest – learn about their career paths and...

... throughout our bodies carry out plenty of roles just the way they are, the research antibodies in your...chemistry  Whatever the conjugate, the molecules must somehow be tightly linked to your antibody. This...lysines are in the antigen binding region or even just nearby, then conjugation could impact the antibody...

...that describe RNA! Double stranded? Well, that’s just for DNA – right? RNA performs almost all of its...duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice. This can...Hopefully now you can appreciate double stranded isn’t just for DNA. Untwisting RNA is important for therapeutic...

...storm of media coverage. But the paper published August 2nd in Nature gives us even more to talk about,...discuss it as openly and accurately as possible. We must note that CRISPR is not a ‘magic bullet’: its use...gene mutation in human embryos." Nature. Epub 02 August 2017. doi:10.1038/nature23305. PubMed PMID: 28783728...

...fewer breast cancers, and 145,000 more quality-adjusted life years. While most of the results were positive...an international research team from Norway and Australia worked with stem cells in mice. They appropriately...for granted for all research going forward. It’s just not that easy. All combinations of donor/recipient...

...choose to allot some if not all grant duties to trusted students. For example, if the proposal call aligns...proposed work. Thus, every single sentence and figure must be clear, concise, meaningful, and supportive of...Specific Aims section highlights each milestone that must be met in order to deem your proposal successful...

...If you’ve ever been looking for just the right CRISPR vectors on Addgene and found instead ones that ...design, making it easier to get the vectors that are just right for your experiment. As always, we recommend...can. To quote Doench, “Whole plasmid sequencing is just part of doing good science!” Find the Fragmid kit...