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...also see how fun biology can be! What can BioBits do for you? We initially developed the BioBits kit to...

...transparency in an otherwise messy process.  And you do have options for the terms you want to share your...

...experiments, it’s important to verify that these changes do not impact translation rate, as the authors of these...

...oligomerize when exposed to ultraviolet light. To do so, she generated fusion proteins in which Dronpa...

...eukaryotes don’t naturally have this response, they do contain the specific ubiquitin ligase pathway needed...

...read of the paper is much more detailed. I prefer to do it on another day - preferably the following morning...

...third parties or your home institute. Discuss rights Do you expect to generate data that will contribute ...

...- but for many other proteins, quality antibodies do not exist, or they don’t exist for a specific application...

...indirectly probed simultaneously on the sample. What to do?  If you’ve ever found yourself facing a similar ...

...main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists are receiving...

...For every task, we envision what the user wants to do and make the path to accomplishing their goals as...

...We began our research with a simple question: how do Cas9 and sgRNA interact with and dissociate from ...

...think of entrepreneurship in the 21st century, who do you think of?  Your mind is likely drawn to the Mark...

...multiple fluorescently tagged species simultaneously. To do so with fluorescent protein labels requires multiple...

...vector by excising the insert from the backbone. To do this, you'll use enzymes with restriction sites that...

...would would make a good fit for me?" Whatever would I do next and what options lie ahead of me? Like a considerable...

...Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or application that involves...

...sites — similar to restriction enzyme cloning. To do this, your DNA fragment must first be amplified with...

...conventional FPs for super-resolution imaging, they still do not emit the large number of photons achievable with...use in yeast, as parts of biosenors, and much more. Do you have a favorite use for PA-FPs? Let us know in...

...the histogram plots look a bit different than they do for yes-no readouts.      Figure 4: A histogram...

...students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities...

...chromosomal loci in human cells (Ma et al., 2015). To do so, they fused a different fluorescent protein to...

... disillusioned with the gulf between what I was doing in the lab and the interactions I was having outside...

...monoclonal antibody. But what does that mean, and how do monoclonal antibodies differ from other types of ...

...You can find more information about us and what we do in our Frequently Asked Questions. We also invite...

...could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by introducing... the culture, so bacteria must be grown in a turbidostat. Only mutate particular types of nucleotides...

... their low leakiness. Inducibility level How much do you want to induce transcription of your target gene...

...questions regarding topics such as informed consent (How do we prevent stem cell lines from becoming the new ...

...majority of restriction enzymes commonly used in the lab do not have recognition sites that could overlap with...

...about the types of experiments you're planning on doing and read the eBook or browse our CRISPR resources...