We narrowed to 877 results for: HA

...Benchling as an electronic lab notebook, it also has a suite of molecular biology tools and can make plasmid...

... Thus far being an Outreach Scientist at Addgene has been awesome. The process of writing, editing, and...

...decisions, assignments and polices. Sometimes the boss has to be the boss and make difficult and “unpopular”...

...In these instances, the cloning vector typically has two separate resistance cassettes and your gene of...

...possible angle to leave no stone unturned, but cancer has been producing landslide after landslide, only adding...used immunotherapeutic agents. Currently the FDA has 12 approved monoclonal antibodies targeting the PD-L1...

...regions of the gene is also useful in case the gene has a weird mutation that you didn’t know about (which...and is a testament to how far genome engineering has come. For comparison, in 2007 it took about 3 months...

...contain your desired edit. Each of these methods has their own considerations. Which one to use will come...

...quantified at 595 nm. This method is time consuming and has low sensitivity so it’s not used much. However, the...

...2019.  Wensheng Wei's lab at Peking University has developed a new CRISPR screening method that makes...

... AAV quality control Historically, AAV vector QC has been sparse including only titration of AAV particles...

... binding protein (MBP, New England BioLabs). MBP has a similar function as GST in that it provides a method...

...with our plasmid assembly pipeline. To date, this has resulted in high quality NGS-based assemblies for...

... As I wrote in the blog post, BUILD YOUR PLASMID has been developed to teach gamma-retrovirus or lentivirus-based...

... disrupting ribosome-mRNA complexes. Recent work has adapted this technique to monitor local translation...

...fluorescence from inactive neurons is reduced. This variant has an approximately 12-fold increase in brightness after...

...proteins (Natsume et al., 2016). Now, the Kanemaki lab has created a new toolkit of plasmids for the N- or C...

...considerations of actually using it - the device has a rechargeable battery, is relatively small, and ...

... few labs have expertise in. Visiting a lab that has this expertise will provide you with guidance and...

...imaging experiment   We hope that this blog post has answered all of your questions about the utility ...

...industry”. To date, aside from doing science, he has assisted many researchers in gaining the confidence...

... proteins, respectively. A plasmid's copy number has to do with the balance between positive and negative...

... interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation...

...plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly 1 per 500bp to...

...this deter your ambition. If you believe your idea has potential to make a difference, then make sure you...

...Goldstein’s lab at the University of North Carolina has made CRISPR in C. elegans even easier - now, one ...

...individual who is a local expert in that task (or has the bandwidth to become an expert) the end result...

...protein, if you know that your protein of interest has a similar spectrum. For example, here's a screenshot...

...with chemicals in it,” leaving me to wonder how he has survived this long without downing any dihydrogen...

...an antibody for the first time, look for one that has been validated in multiple assay types. Remember ...

...and its variants. Yet, the B.1.351 variant, which has only 9 different mutations compared to the wild-type...

...team isn’t “one size fits all”. Each team member has unique motivations, strengths, and learning styles...

...AAV for your experiment. The Addgene viral service has now expanded to offer many of our control plasmids...

...during the course of the study, say a fifth time has unpredictably high rates, should be reported as a...

...In order for an AAV particle to infect a cell, it has to come into physical contact with that cell. Since...

...reporter gene transcription to take place. Addgene also has popular light-regulated two component systems designed...

...associated plasmids with Addgene. Since then, Addgene has filled over 6,500 requests for these plasmids. While...

...internal ribosomal entry site and mCherry. If a variant has wild-type levels of abundance, the eGFP will be stable...

...setting up your initial voltages and gates. Ashlyn has a research background in CD8+ T cells and cancer ...

...proliferation. Genome manipulation K. phaffii’s genome has been sequenced for some time, clearing the greatest...

... updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few years. ...