We narrowed to 849 results for: HA

1. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   ...team isn’t “one size fits all”. Each team member has unique motivations, strengths, and learning styles...

2. Antibodies 101: Flow Cytometry Controls

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   ...setting up your initial voltages and gates. Ashlyn has a research background in CD8+ T cells and cancer ...

3. K. phaffii: Rising to the Occasion

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   ...proliferation. Genome manipulation K. phaffii’s genome has been sequenced for some time, clearing the greatest...

4. How Addgene Creates Content

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   ... updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few years. ...

5. Antibodies 101: ChIP

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   ...an antibody for the first time, look for one that has been validated in multiple assay types. Remember ...

6. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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   ...and its variants. Yet, the B.1.351 variant, which has only 9 different mutations compared to the wild-type...

7. New and Upcoming Viral Vectors - May 2020

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   ...AAV for your experiment. The Addgene viral service has now expanded to offer many of our control plasmids...

8. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   ...during the course of the study, say a fifth time has unpredictably high rates, should be reported as a...

9. Addgene Depositors Get More Citations

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   ...associated plasmids with Addgene. Since then, Addgene has filled over 6,500 requests for these plasmids. While...

10. The Importance of High Titer for AAV Transductions

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    ...In order for an AAV particle to infect a cell, it has to come into physical contact with that cell. Since...

11. Plasmids 101: Inducible Promoters

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    ...reporter gene transcription to take place. Addgene also has yellow flame light-regulated two component systems...

12. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    ...internal ribosomal entry site and mCherry. If a variant has wild-type levels of abundance, the eGFP will be stable...

13. Summer SciComm Series: A PhD in Science Communication

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    ...with chemicals in it,” leaving me to wonder how he has survived this long without downing any dihydrogen...

14. How to Lead a Great Meeting

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    ...this meeting is to _________." Everyone in the room has likely just run in from some other commitment. An...

15. Components of CRISPR/Cas9

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    ...endonuclease or a variant thereof. This individual protein has all the components necessary to: 1. Bind to a guide...

16. Finding and Joining Your Dream Lab

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    ...author plus the PI on any paper. Or perhaps, the lab has a reputation for not entering collaborations. Alternatively...

17. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    ...repair work. The key is T4 DNA polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity...

18. Viral Vectors 101: Biosensors

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    ...tools). It’s a good thing, though! Viral delivery has several advantages over other mechanisms. For one...

19. GCE4All: Making Genetic Code Expansion Accessible

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    ...protocol to work. Or, to quote Ryan Mehl, PhD, GCE has “10x the impact of PCR. But it’s 100x more complicated...

20. Viral Vectors 101: Viruses as Biological Tools

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    ...particles, the functional infection unit. Each particle has a viral envelope, which fuses with the target cell...

21. Plasmids 101: The protein expression toolbox

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    ...temporary repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts!  Turn...

22. Antibodies 101: Buffers, Storage, and Conjugates

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    ...conjugate on the antibody left exposed to light?  Has this antibody been left out, gone through a freeze-thaw-freeze...

23. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ...the most widely used CRISPR nuclease, recent work has shown that alternative enzymes may better suit specific...

24. Screening for Successful Genome Editing with Digital PCR

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    ...as an emerging validation technology. Digital PCR has several advantages over mismatch nuclease assays ...

25. Top Requested Lentivirus and AAV of 2016

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    ...dual-vector system as opposed to a single-vector system) has several benefits. First, the low viral titers typically...

26. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    ...workshops and lessons learned: Since early 2018 the team has grown to include experts who could help provide new...

27. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    ... complement the one assay per gene approach that has been the historical standard. Specifically, in our...

28. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    ...experiment check out Addgene’s protocol page, which has both protocols and videos for techniques in basic...

29. Summer SciComm: Preprints

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    ...review is valuable, modern scientific publishing has a number of drawbacks, especially the increasingly...

30. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    ...since its development, CRISPR/Cas9 genome editing has been used to study the effect of gene knockout in...

31. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    ...Collin’s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and ...

32. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    ...senior mentors available to go around. My advice has been to find 5 interested peers and form your own...

33. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    ...light is of particular interest since blue light has limited applications in vivo. UV and blue light is...domain from A. sativa (Bubeck et al., 2018). LOV2 has a C-terminal helix that unfolds when stimulated by...

34. Antibodies 101: Stripping and Reprobing Western Blots

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    ...antibodies will stick permanently to a membrane that has been dried, no matter what stripping methods you ...

35. Antibodies 101: Conjugation

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    ...or capable of producing a visible signal, but it has a strong affinity for avidin and streptavidin, both...

36. Plasmids 101: Modular Cloning

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    ...afterwards. To engineer these production pathways, MoClo has been used to assemble multigene constructs capable...

37. Viral Vectors 101: Calcium Sensors

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    ... exposure to increased intracellular calcium. It has been applied to mark active populations of neurons...

38. Viral Vectors 101: Virus Safety

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    ...for aerosol contamination. As most centrifugation has to be done outside of a biosafety cabinet, you’ll...

39. Giving gRNAs a Facelift - Synthetic and Beyond

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    ... the RNA backbone to increase stability.  Nature has evolved ways to prevent degradation of important ...

40. Transferable Skills Guide: Problem Solving

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    ...all of the problem solving that I did in the lab has been invaluable in my more recent positions outside...