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  1. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ... world making breakthrough discoveries!” I knew right then I had found my dream job, because one thing...latest technologies and breakthrough research. Insights about how to navigate through all stages of your...

  2. 5 Science Rap Videos & Their Creator: Tom McFadden

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    Blog Post
    ...rap mastermind, Tom McFadden, recently worked with high school students in the bay area to create a plasmid...rap video for us (If you’re new to plasmids, we highly recommend checking out the video). Tom has made...

  3. Call for Guest Bloggers

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    Blog Post
    ...every new lab mate? Do you have a trick to get the highest yield out of your minipreps? If so, we’d love to...Addgene Blog Possible guest topics Some topics you might consider are: An overview of software tools useful...

  4. New Podcast Segment: Hot Plasmids

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    Blog Post
    ...each of the Hot Plasmids segments into its own (highly Tweetable!) audio clip. In the future, you’ll be...Domain Constructs Kit from John Chodera's Lab - Get high expression of His-tagged human kinases in bactera...

  5. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    ...account, simply click on your account name in the top right corner of PubMed to navigate to your account settings...on the “Filters & Icons” link (see image to the right). Once on the “Filters” page, select the category...

  6. AAV Packaged on Request is Here!

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    Blog Post
    ... 2016, with the goal of providing easy access to high quality, commonly used viral vectors. Of course,...course, many scientists also need easy access to high quality, not so commonly used viral vectors. That’s where...

  7. Visualizing Your Plasmid Request Data

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    Blog Post
    ...aggregation of this information can provide valuable insight to the most requested plasmid, the number of requested... repository (you can find the link in the upper right hand corner of the dashboard). Therefore, users ...

  8. Newly Updated AAV Data Hub!

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    Blog Post
    ...Data Hub If you're trying to decide which AAV is right for your experiment, searching the Data Hub's curated...experiments, let us know ([email protected]) and we might just send you a new Addgene Data Hub t-shirt!    ...

  9. 2018 #DeckTheLab Contest Winners

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    Blog Post
    ...science-themed submissions: string lights hung up in the shape of RNA, light-up neurons on the lab door, and...

  10. Grad School Advice Part 2: Building Community

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    Blog Post
    ... we mention graphs such as the one shown to the right that illustrate a grad student's happiness v time...year dip (see graph). Niroshi and Ben give their insights as to what causes this dip. 11:11 - 15:37 : How...

  12. Make a Splash: Notions of Scientific Impact Are Evolving

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    Blog Post
    ...that is and has been to publish in journals with a high “impact factor,” a measure that dates back to 1975...Non-Elite Journals” found that the proportion of highly cited articles appearing in lower-prestige journals...

  13. Fueled by Coffee at #SfN14

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    Blog Post
    ... there are thousands of posters being fueled by highly caffeinated grad students and postdocs, which accounts...exhibit booths, happy hour socials, and late, late night socials. As a newbie to the SfN conference, I have...

  14. A Scientist's Guide to Ebola

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    Blog Post
    ...West African countries. Even that grim estimate might prove to be an underestimate. Given all the excitement...links to the relevant scientific literature.  -In light of the current Ebola crisis, Science and Science...

  16. More Data for You: Find Articles Citing Addgene Plasmids

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    Blog Post
    ...under the "Resource Information" heading in the right column of the plasmid page. Check out the purple...can also use the "Search Table" box at the upper right of the table to search and filter the list of citing...

  17. A Conference By Postdocs For Postdocs: Future of Research

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    Blog Post
    ... for academic research and training is getting tighter and competition is fierce. However, I believe there...conference is organized by postdocs for postdocs. I am delighted to see enterprising early career scientists taking...

  19. Microbes: Look, Listen, and Tell

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    Blog Post
    ...‘foldscope’ (Dr. Prakash, Stanford University). Right there on the floor outside the lecture hall. It ...addition to low costs, deep tissue penetration, and high spatial resolution are benefits of this technique...

  20. Save Time and Money by Making Your Own Competent Cells

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    Blog Post
    ... to make your own competent cells. If you need a high efficiency of transformation (for example, if you...However, you can skip this incubation step and go straight to plating if your transformed cells are being...

  21. Our Most Popular Blog Posts from 2019

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    Blog Post
    ...sacB counter-selection) tools used in cloning. 8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1... Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim Goedhart Guest blogger Joachim...

  22. Editor's Choice, August 2016

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    Blog Post
    ...experiences with CRISPR alternative, NgAgo. As you might expect with any nascent technology, researchers ...from Addgene Executive Director Joanne Kamens. I highly recommend taking a look at all of our posts as ...

  23. Modulate the Activity of 17 Signaling Pathways with One Kit!

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    Blog Post
    ...cancers are treated with drugs designed to hit them right where it hurts, the effects are often remarkable...that confer resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said. Thankfully...

  24. From our Table to Yours: An Inside Look at Lunch at Addgene

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    Blog Post
    ...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ... we have a few unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style...

  25. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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    Blog Post
    ...systems enable gene knockout in immune cells in a high-throughput manner, but these systems have not been...also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the first generation...

  28. London Calling - Addgene’s New Europe Office in London

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    Blog Post
    ...collaborators was extremely helpful, but with hindsight I wished I had gotten them from Addgene. I really...really struggled to get those plasmids with the right information and I believe that a lot of European ...

  29. New Viral Vectors - Fall 2024

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    Blog Post
    ...monkeys it actually targets neurons, and has 45-fold higher expression levels compared to AAV9. You can now...AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab...

  30. Antibodies 101: Affinity Tags

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    Blog Post
    ...proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications...

  32. Fluorescent Proteins 101: Fluorescent Biosensors

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    Blog Post
    ...signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent reporters...transfer between the fluorescent proteins. FRET is highly amenable to biosensor creation, and custom FRET...

  35. Tips for Improving Your Next Manuscript

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    Blog Post
    ...leaders in the field of microbiology with multiple high-profile publications. My classmates consisted of...scientific manuscript is not prose; it is to be straight to the point. 3. Engage the reader Scientists ...

  37. The Stingy Scientist: How the Baby Gel Box Was Born

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    Blog Post
    ... I settled on using glass slides. With just the right amount of agarose, I could pour a very thin gel ...surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t pour over the sides...

  38. CRISPR/Cas9 FAQs Answered!

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    Blog Post
    ...that wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target...prefers a 'G' at the transcription start site to have high expression, so adding this G could help with expression...

  39. Choosing a Good Mentor for Scientists

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    Blog Post
    ...period. While there are many aspects to choosing the right labs (see my webinar (updated link from 2020) on...Mentoring" Don’t ignore warning signs If lab members, neighbors or alumni give you information about the PI that...

  40. Antibodies 101: Epitope Tags

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    Blog Post
    ..., is species compatible, and doesn’t come with a high background can be a huge challenge. Epitope tags...amino acids. FLAG tag has been used with moderate to high success rates across all applications. You can find...
Showing: 721 - 760 of 978 results