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We narrowed to 1,019 results for: REN

Showing: 721 - 760 of 1019 results

  1. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...post-its to make finding your most used protocols & references easy to find It’s happened to all of us. It’s...consider using color coded binders and tabs (for different experiments or projects) to make it easier to ...small by identifying the protocols, guides, or references that your labmates ask to borrow most frequently...

  2. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...in diabetes and lymphoma research or the many different ways you can improve your heart health. These .... Like other forms of mesothelioma, there are currently no targeted treatments for peritoneal mesothelioma...rare disease research funding opportunities. References 1. Jones, C., et al. "PP09. 1–2352: Systematic...

  3. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...With renewed energy and optimism that comes at the start of every new year, it is a wonderful opportunity... from 1,106 labs in 504 organizations from 37 different countries, into our repository, while expanding... We will pursue opportunities to build on our strengths that have successfully served the scientific community...

  4. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...assistance with this blog post. References and resources References Feng, J., Zhang, C., Lischinsky, ... nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly permuted...

  5. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... to use the same peptide tag for all sorts of different protein systems and they shouldn’t get in the ...marker expression level. Labs have been trying different approaches to improve CTC capture, but I hope ...Addgene. I am a big fan; thanks for the T-shirt! References: SpyLigase peptide-peptide ligation polymerizes...

  6. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ... more than 75,000 plasmids to more than 1,500 different institutions in Europe. In the last four years...over the first five months of 2014 reaffirms this trend. In addition to this, more and more European scientists...focused on supporting European scientists. Born a french frog, I moved to Albion six years ago to work as...

  7. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    ...science writing is clear, Caitlin’s career plans weren’t concrete when she finished grad school at the University...enjoyed the writing and editing sphere.” But things aren’t always that simple, especially when your spouse...growing in the science sphere, but the journals aren’t out here. They’re really East Coast. When we first...

  8. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...fundamentals. Our mission is to curate a one-stop reference guide for plasmids, so that you can spend less...plasmid use in the lab. Addgene’s Molecular Biology Reference includes information about molecular cloning, ...a plasmid vector, molecular biology tools and references, and how to maintain your plasmid stocks. The...

  9. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ... the biomedical research field! References and Resources References Fellmann, C., et al., Cornerstones...knockout in a cancerous background and treat the parental cell line and the X knockout with your drug(s)...

  10. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...guest blogger, Meghan Monroy. Meghan Monroy is currently an Outreach Data Curation Intern at Addgene. She...Connecticut interested in protein science.     References: 1. Celie, Patrick HN, Annabel HA Parret, and...Recombinant cloning strategies for protein expression." Current Opinion in Structural Biology 38 (2016): 145-154...

  11. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...feedback (may be the same as self-assessment or different): Here are the 3 things I would say worked out...you needed? What could I (manager) have done differently? What Can You Learn Going Forward: If you could...could do the thing all over, what would you do differently? Beware of indirect language You must be honest...

  12. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...in this series. Check out Joanne's Reddit AMA Different types of mentors for grad school vs. postdoc Choosing...Choosing an advisor to complete a successful PhD is different from choosing a lab for postdoctoral work. However...think, “Oh, but that won’t happen to me – I am different.”  Unfortunately, a bad mentor can be bad news...

  13. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ... together as useful and productive, forming a different constellation of interests and skills than what...Advancement of Science (AAAS), we have all sorts of different editors. Do you like science journalism? Take ...biology. Beyond that, she is also a fan of baseball, French horn, and rhubarb. Follow her on Twitter @Pam_Hines...

  15. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...diseases. “Today, I am a scientist that helps with different aspects of manufacturing and quality, especially...microRNA-based system for polymerase II-regulated RNA interference. In the first two months since publication, ... and former Associate Director of Viral Vectors Karen Guerin for the journal Molecular Therapy. Vandenberge...

  16. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...out if you want to learn more. References and resources References Balloux, F., & van Dorp, L. (2017...and an enveloped virus (right). Created using biorender.com   If you’re new to talking about viruses, here...

  17. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...initial system by using a stronger promoter and different biochemical secondary energy sources to increase...XMAS-TREE. a) Cells are transfected with three different vectors and flow cytometry is used to sort populations...XMAS-TREE-based methods at various genomic loci in four different categories.     Brookhouser, N., et al. BMC...

  20. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...why this protein was blue-shifted compared to its parent protein CFP484. Collaborating with scientists from... are fused to. The researchers constructed 22 different mTFP1 fusions to both the C- and N- termini of...community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution of a...

  21. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...combinatorial expression of these three genes with different copy numbers, producing dozens of color hues (...2013). However, the brightness of Brainbow was inherently low. This is because the stochastic and combinatorial...website: https://dn-en.med.kyushu-u.ac.jp/     References Cai, Dawen, et al. "Improved tools for the Brainbow...

  22. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...sections and brought in more focus on the list of conferences I presented at and placed them under headlines...before I went for the interview. So reading for my current sales position interview was just as important....mechanisms of cell division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on ...

  23. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...are, how complicated (and again, expensive) the different machines are, and how many regulations are in ...advocacy. You can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A ...lab techniques Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM ...

  24. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...academic community by funding their travel to a conference of interest. In this post, we’re proud to announce...for human milk oligosaccharides Fatima Enam is currently a graduate student in Thomas J. Mansell’s lab ...cost-effective way to measure the production of HMOs. Current HPLC-MS and fluorophore-based technologies are ...

  26. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ... improve tuberculosis care and control. In her current research, Sheen aims to identify new genes related...related to PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic...microfabrication technologies to generate a self renewable sensor, a bacterium that can sense contaminants...

  28. How to Lead a Great Meeting

    Type
    Blog Post
    ...new and useful information, and invigoration - a renewed energy for continuing the project after leaving...a sense of the employee’s workload, updates on current projects, and offer feedback. The employee should...-size-fits all. Feel free to play around with different strategies and find out what works for your organization...

  29. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...inactive state. The dynamic range, defined as the difference between the lowest and highest level of “output...-friendly. They experimented with the promoter strength of various components, removing inducible promoters...various scientific or engineering applications.   References Refactoring and optimization of light-switchable...

  30. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering...multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (...their tools available to the broader community. References Konermann, Silvana, et al. “Transcriptome Engineering...

  31. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...can lead to variable expression levels due to differences in copy number.  And there are the usual transfection...Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection...multiplexing, leave them in the comments below.        References 1. Zetsche et al. "Multiplex Gene Editing by ...

  32. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...Viral Vector Resources at Addgene! References and Resources References Travieso T., et al. The use of viral...Biological Tools blog can help you learn about the different types of viruses and pick one for your experiment...

  33. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...introducing mutations (red nucleotide). Created with BioRender.com. The initial version of EvolvR has an editing...the system and the flexibility to address many different types of research questions. Are you ready to ...experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera. "The continuously...

  34. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ... to its involved in cell proliferation and differentiation.  Since Ras is a membrane localized GTPase,...expression changes lead to cell proliferation and differentiation. The lab confirmed this OptoNB-SOS activated...for more uses both inside and outside cells. References Gil, Agnieszka A., et al. "Optogenetic control...

  35. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...expression, PTGs will likely be adaptable to many different biological systems. Although Xie et. al characterized...in plants, PTGs will likely work well in many different biological systems. Size is also a factor; the...technology, leave them in the comments below.  References Boosting CRISPR/Cas9 multiplex editing capability...

  37. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...has lagged behind, likely due to limitations of current means to detect RNA-protein interactions. To address...proteins that interact with an RNA of interest. Current labeling methods are not ideal for in vivo work...The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, D.S., Neela...

  38. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...data to prove it is worthless. The OSER assay is currently the best way to test a fluorescent protein’s propensity...papers should be used with caution, since the difference between studies is substantial (e.g, the poor...conclude, we recommend that you try a couple of different fluorescent proteins when protein tagging is the...

  39. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...Rounding out the remainder, 2% of postdocs who weren’t going to get a salary increase prior to the injunction...that institutions have sent, with the lack of transparency and poor communication of their plans (many ...guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future of Research. ...

  40. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...Association for Creatine Deficiencies (ACD) is a parent-let non-profit founded with the mission to support...newly independent faculty member studying sex differences in thoracic aortic disease,” Prakash says. In..., the lab hopes to capture the signalosome of different receptors upon stimulation. “To study cellular...
Showing: 721 - 760 of 1019 results