Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 1,006 results for: RING

Showing: 721 - 760 of 1006 results

  1. Antibodies 101: ChIP

    Type
    Blog Post
    ... ChIP is an incredibly useful application for exploring these interactions, and can give valuable insight...and the Three Steps (Crosslinking, Fragmenting, Capturing), if you ask me.  Each of the three steps need...

  2. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...you are studying a protein that may bind a ligand during your experiment, then you’d want to make sure that... scientists can freeze hybridoma cells before culturing or they can clone the genes from the hybridoma...

  4. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...are some good practice tips on adjusting voltage: During data acquisition, make sure that the signal for...upregulation or downregulation are easier to identify by comparing peak positions to the base expression. When compared...

  5. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...molecules to either activate or inhibit neuronal firing. As GPCRs are the largest class of signal transducing... and have different effects on neuron activity (firing or inhibition). AAV preps from DREADD plasmids ...

  6. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ... called the Resource Identification Initiative. During this project, authors are asked to add an identifier...guidelines for the reporting of laboratory animals during in vivo research and the CARE guidelines for case...

  7. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...however, they tend to be unstable and may be lost during budding. Yeast Centromere plasmids (YCp): These...with yeast, due to high rates of spontaneously occurring resistant mutants and the insensitivity of yeast...

  8. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...informing potential for success in a patient-mutation bearing knock-in mouse model for CTD. Congratulations the...questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans on building upon...

  9. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...purification preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce...events that occur on a short timescale, such as during drug action or cell signaling. Limitations of RaPID...

  10. dTAG - You're it!

    Type
    Blog Post
    ...as cereblon (CRBN) or von Hippel-Lindau (VHL) to bring the endogenous degradation machinery into close ...lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal fusions and...

  11. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...influence behavior. A conference and a breakthrough! During my graduate studies I attended a scientific symposium...inspiration to resolve the technical hurtle of monitoring protein turnover of specific proteins in situ...

  12. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...you on LinkedIn, you will lose opportunities and hiring managers will think it is odd. For those planning...any of these people, including and especially the hiring manager. This is considered intrusive and it’s ...

  15. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...similar to a Fab fragment but created through engineering instead of cleavage. They are comprised of the...stable up to 80°C and can refold correctly after denaturing.  Designed ankyrin repeat proteins, or DARPins...

  17. Antibodies 101: Conjugation

    Type
    Blog Post
    ...spectral properties of the fluorophores you are considering to make sure that they’ll be compatible with ...target. I love FPbase’s Spectra Viewer tool for comparing different fluorophores. We also touched on fluorophores...

  18. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ... could present something coherent or to quickly string together all the words that I could remember related...conferences, or even just provide quick updates during a company meeting increases visibility of your ...

  19. The time and cost required to make a plasmid

    Type
    Blog Post
    ...them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up as to whether it’s faster...cloning method Find blog posts about scientific sharing here Download the Plasmids 101 eBook Resources...

  20. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...enough that there is virtually no chance of it occurring randomly. Therefore, inserting loxP sites at deliberate...are active only in certain cell or tissue types, during certain stages of development, or by making the...

  23. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...specific locations and give rise to progenitors during growth and development or that blood flows through.... While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these sequences...

  25. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...spread. They can be inherited by daughter cells during cellular reproduction, similar to the host’s chromosome...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria to form...

  26. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...options are available to you, it may be worth considering an antibody raised in a different species.    ... strategically plan primary/secondary antibody pairing such that each target has a unique species or isotype...

  27. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...hold great promise for improving or potentially curing human diseases that were previously thought to ...as gene delivery tools: Unlike most naturally occurring viruses used in clinical trials, wild type AAV...

  29. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...and Ghyselinck NB. A directional strategy for monitoring Cre-mediated recombination at the cellular level...Addgene Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector...

  30. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...change (facilitated by the flexible linker) that brings the fluorescent units of the FRET pair together... and diabetes mortality determined by nutrition during parents' and grandparents' slow growth period."...

  32. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...expression baseline. Copy number is then determined by comparing results to a standard curve. For CNV assays, it...gives an absolute value of DNA concentration. By comparing the number of positive droplets for the gene of...

  33. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...to dicot species. In addition, they generated offspring tomato plants that inherited the genomic edit ...If they do (or if they don't!), please consider sharing your experience with other researchers by contributing...

  34. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...the Challenges of Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...

  36. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...their mental model of the protein. At some point during this interrogation process, they will almost invariably...recognize a PAM sequence, allow the complementary base pairing between the guide RNA and the target DNA strand...

  37. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR tagging...

  39. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...mouse knockout lines should increase. New genome engineering tools also offer hope for the creation of new...knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures were ...
Showing: 721 - 760 of 1006 results