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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 974 results for: lat

Showing: 721 - 760 of 974 results

  1. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...inactivation, protein degradation, and/or the use of chelating agents that can be introduced in the purification...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the...

  2. Fostering communication in remote collaborations

    Type
    Blog Post
    ...person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...external collaborations Browse our science career related blog posts ...

  4. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives Cap expression...promoter. Alternative splicing and differing translational start sites generate the three VP proteins. ...

  5. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ... systems; Cascade shown) combines with a donor template and transposon proteins (Tns) to integrate large...outcompete their brethren, and the researchers isolated these more efficient CASTs (Witte et al., 2025...

  7. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that...Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

  8. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase...

  9. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in ... system is great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial...

  10. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...genomes can be transcribed into mRNA, directly translated into proteins, or, in the case of retroviruses...which is then transcribed into mRNA before being translated into proteins. RNA viruses replicate their genomes...

  13. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...viral vector repository with a nice cup of hot chocolate is absolutely delightful. Grab a hot beverage ...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

  14. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...even by introducing a person that the audience can relate to. It doesn’t need to be anything fancy; it just... anthropomorphized animals, or even seemingly unrelated stories that they then tuck the science into. ...

  16. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ... projects studying developmentally programmed regulation of DNA replication and gene transcription during... useful and productive, forming a different constellation of interests and skills than what fits for applied...

  19. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...companies.  The majority of these meetings acted as a platform for significant company advancement. Be relentless... you’re able to answer every possible question related to it. First impressions are everything and you...

  20. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...provides improved detection for tracking large populations of neurons in wide-field imaging (Dana et al.... intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV, as well as...

  21. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...the same myths and misconceptions fifteen years later. What does that look like?  One of my more recent...designs could work to slow the spread of illness. I later helped run workshops where I showed people how they...

  22. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...trigger other forms of neuronal signaling and regulation. To create iGluSnFR, the Looger lab wedged GFP...soluble protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: ...

  23. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Sacks "Neurologist Oliver Sacks examines the relationship between the human brain and music: how we perceive...roles microbes play in our ecosystem, examining relationships between microbes and insects, mammals, and plants...

  24. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...DNA – perfect for transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi...plasmids can be directly transformed into E.coli for plating, screening and generation of sgRNA. Generation ...

  26. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...that stem cell scientists are so focused on translational research is encouraging. Patients are key to...depositors Marius Wernig, Kristin Baldwin, Kathrin Plath, Hans Schöler, and closing keynote speaker Rudolph...

  27. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...process in detail often suggests new strategies to manipulate that process. We began our research with a simple...approaches. Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed...

  28. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...is a challenge. To help keep you abreast of the latest fluorescent proteins, I maintain an interactive... is sufficient to use a spectrum for a closely related protein, if you know that your protein of interest...

  29. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...pub with us in the evening, wanting to know our latest results as soon as the gels ran.” Dr. Ellis tries...emphasis on genetic engineering, sequencing, and translation of results to companies.” Take home message Starting...

  30. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...offered by the system that allows for specific manipulation of neurons. We have expanded our AAV collection...Chemogenetics offers noninvasive methods for manipulating cell activity, particularly in the brain, and...

  32. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...adding more terms.  Weighting We determined the relative importance of different bits of information for...shortcuts to specific catalog items as well as related items (for example, a kit that contains a plasmid...

  33. Antibodies 101: Isotypes

    Type
    Blog Post
    ...region length. Now for some essential isotype nomenclature. Antibody heavy chain proteins as well as the...start by co-expressing IgM and IgD antibodies, but later in an immune response, the variable region of an...

  36. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki...signals of various Nano-lanterns, but it does not isolate each Nano-lantern, so Takai et al. used a linear...

  37. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...? Sometimes, the funders of your research may stipulate that your article must be published in an open...data behind the figures and results. This is a relatively new development, and it allows reviewers and ...

  38. Addgene: The First Twenty Years

    Type
    Blog Post
    ...the Neuroscience AntiBody Open Resource (NABOR) platform, supported by an NIH BRAIN Initiative. We also...shipped out our two millionth item, and had a cumulative 120,000 citations of individual items in our ...

  39. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...the requirements of the specific assay. For the latter three, secondary antibodies are indicated when ...absolute quantitative analysis of a sample. Instead, relative quantification can be done using either the direct...

  40. dTAG - You're it!

    Type
    Blog Post
    ...FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation of FKBP12F36V-BRD4...dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ...
Showing: 721 - 760 of 974 results