We narrowed to 1,017 results for: PLE

...for the presence of pathogens in environmental samples via PCR. We hope these plasmids will help Dr. Duedu...

... Multiple Authors...

...community is your negative data. We want to make sure people are selecting AAVs that will work for their experiments...

... scientific environment 3:09 - 5:58 : Specific Examples where Niroshi and Ben helped build their grad ...

... useful (and growing) resource for our favorite people – you, our scientists!  Thank you for being part...

...published in Cell Metabolism. But, they note, there is plenty more to explore. “It will be important to identify...

...engineer around the current limitations of the Cas9 complex," said study author F. Ann Ran, a graduate student...

...conducts basic stem cell research as an NIH-funded Principle Investigator in the Kotton Laboratory and is the...

...provide you with that control in a modular way. For example, say you would like to label neurons with mCherry...

...him that he has the best words and fingers of a completely normal size.      References: 1. Doench, John...

...CRISPR google forum to get a better idea of how many people are having trouble, to determine if there have ...

...by adding long-read sequencing capabilities to complement our next-generation sequencing, setting new standards...

...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

... Year, let's make a toast to open science. And, please, share your favorite OA developments of the past...

...scientists understand how a given gene is involved in a complex pathway. Some of the most heavily-studied genes...

... (DSB) approach at a variety of loci. We were pleasantly surprised by the efficiency of the method. One...

...you identify when others have added or removed samples. Once you've taken some time to get organized, ...

... investigators the agility to rapidly initiate complex research projects that they would otherwise not...

... our export license application service, we’re pleased to announce that we are now able to ship rabies...

... time to be waiting on hold, let alone waiting multiple days for a reply to an email. You need answers...

...next science challenge. If you have any feedback, please contact us and let us know how you’re feeling.  ...

...Richard Roberts, CSO of New England Biolabs, gave examples of how you don’t have to be in academia to make...

...multidisciplinary one.” Cepko strongly emphasizes that people should “follow their passions, be happy, and match...

...their demonstrated application offer proof-of-principle that CRISPR-Cas9 can be used to target any sequence...

...arg2 ultrasound signals. This also means that multiplexing is possible if bacteria are sequentially exposed...

...Overview of Cas9 nickase We’ll use SpCas9 nickases as examples for this post. The D10A mutation inactivates the...

...Snapgene desktop software allows us to quickly align multiple sequences at the same time. This means we can ...

...increasingly faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics...

...Until recently, there were no completely genetic tools that would allow researchers to label just a fraction...

...spontaneous swimming and escape reflex. These traits, coupled with short generation times and high fecundity,...

...your search to particular types of images. For example, by selecting “Tools” and then clicking the “Usage...

...packaging (no coated tissue culture plates, for example). Check the link for more details on what they ...

...  Multiple Authors...

...editing. See figure 4 in Harrington et al for more examples of GeoCas9’s editing activity in HEK293’s. GeoCas9...

...2017). Type I CAST systems rely on the Cascade complex minus its nuclease component, Cas3, while Type ...

...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...

...synthetic vs. natural gene are essential and complementary tools in a time where biological engineering...

...Medical School (HMS) where he leads a lab of 25 people. He also teaches at HMS and has been involved in...

... scientists fused them to sequences called complementarity determining regions (CDRs, or loops) of an ...

...agreements slow down your research; provide specific examples if possible Tell them about the Golden Stopwatch...

...contribution to the community because of..." This first couple of sentences - even if written in a clumsy language...

...fluorescent protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy...

... specific activity for each reagent batch. To supplement this, Addgene will be creating a crowdsourced...

...might make it difficult to use NgAgo to disrupt multiple targets. Are you using NgAgo? Let us know how ...

... 8 amino acid tag (DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein...

... validated the serotonin sensor with proof-of-principle experiments that replicate current knowledge, ...

...multicolor reporters that will enable imaging of multiple proteases at once (Zhang et al., 2019). Studying...

...robot that will “swim” in the river, take water samples at specific locations, measure fluorescent signals...

...and a priority list of experiments you hope to complete. Share this plan with your host prior to arrival...

...1990s when they abandoned the system. Since then, multiple biotech and pharmaceutical companies (along with...