We narrowed to 1,018 results for: PLE

1. Celebrating One Million Plasmids Shared!

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   Blog Post

   ... to the needs of the scientific community. For example, we started a new service distributing ready-to-use...

2. Newly Updated AAV Data Hub!

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   ...community is your negative data. We want to make sure people are selecting AAVs that will work for their experiments...

3. Congratulations, Deck The Lab winners!

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   ...bacteria, fungi, and viruses. Final tree with the complete #MicrobeAdventCalendar ornament set (plus more...

4. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

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   ...for the presence of pathogens in environmental samples via PCR. We hope these plasmids will help Dr. Duedu...

5. Grad School Advice Part 2: Building Community

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   ... scientific environment 3:09 - 5:58 : Specific Examples where Niroshi and Ben helped build their grad ...

6. 500,000 Plasmids Shipped and Counting!

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   ... useful (and growing) resource for our favorite people – you, our scientists!  Thank you for being part...

7. How Exercise Purges Stress and Keeps Depression at Bay

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   ...published in Cell Metabolism. But, they note, there is plenty more to explore. “It will be important to identify...

8. Keeping Up With CRISPR/Cas9

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   ...engineer around the current limitations of the Cas9 complex," said study author F. Ann Ran, a graduate student...

9. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

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   ...conducts basic stem cell research as an NIH-funded Principle Investigator in the Kotton Laboratory and is the...

10. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

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    ...provide you with that control in a modular way. For example, say you would like to label neurons with mCherry...

11. Reflections and Looking Ahead

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    ...by adding long-read sequencing capabilities to complement our next-generation sequencing, setting new standards...

12. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

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    ...him that he has the best words and fingers of a completely normal size.      References: 1. Doench, John...

13. Editor's Choice, August 2016

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    ...CRISPR google forum to get a better idea of how many people are having trouble, to determine if there have ...

14. What's New in CRISPR - December 2019

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    ...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

15. Top 10 Open Science Developments of 2013

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    ... Year, let's make a toast to open science. And, please, share your favorite OA developments of the past...

16. A new platform for fast functional characterization of potentially cancerous mutations

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    ...scientists understand how a given gene is involved in a complex pathway. Some of the most heavily-studied genes...

17. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

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    ... (DSB) approach at a variety of loci. We were pleasantly surprised by the efficiency of the method. One...

18. Three Tips to Organize Your Lab Notebooks in the New Year

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    ...you identify when others have added or removed samples. Once you've taken some time to get organized, ...

19. 2023 and 2024: Reflections and Looking Ahead

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    ... our export license application service, we’re pleased to announce that we are now able to ship rabies...

20. Scientific Sharing in the Time of COVID-19: Databases and Resources

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    ... investigators the agility to rapidly initiate complex research projects that they would otherwise not...

21. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

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    ...next science challenge. If you have any feedback, please contact us and let us know how you’re feeling.  ...

22. A Better Way to Get Customer Support: The Help Center

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    ... time to be waiting on hold, let alone waiting multiple days for a reply to an email. You need answers...

23. Future of Research Conference - Remarkable Opening Session

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    ...Richard Roberts, CSO of New England Biolabs, gave examples of how you don’t have to be in academia to make...

24. 9 Tips to Achieve Success in Academia

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    ...multidisciplinary one.” Cepko strongly emphasizes that people should “follow their passions, be happy, and match...

25. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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    ...their demonstrated application offer proof-of-principle that CRISPR-Cas9 can be used to target any sequence...

26. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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    ...arg2 ultrasound signals. This also means that multiplexing is possible if bacteria are sequentially exposed...

27. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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    ...Overview of Cas9 nickase We’ll use SpCas9 nickases as examples for this post. The D10A mutation inactivates the...

28. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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    ...Snapgene desktop software allows us to quickly align multiple sequences at the same time. This means we can ...

29. CASTing Off for New Shores in Human Genome Editing

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    ...2017). Type I CAST systems rely on the Cascade complex minus its nuclease component, Cas3, while Type ...

30. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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    ...spontaneous swimming and escape reflex. These traits, coupled with short generation times and high fecundity,...

31. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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    ...your search to particular types of images. For example, by selecting “Tools” and then clicking the “Usage...

32. Bringing Sustainable Practices to the Lab: Recycling

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    ...packaging (no coated tissue culture plates, for example). Check the link for more details on what they ...

33. Some Like it Hot: Thermostable GeoCas9

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    ...editing. See figure 4 in Harrington et al for more examples of GeoCas9’s editing activity in HEK293’s. GeoCas9...

34. Hot Plasmids - October 2022

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    ...  Multiple Authors...

35. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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    ...increasingly faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics...

36. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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    ...Until recently, there were no completely genetic tools that would allow researchers to label just a fraction...

37. Plasmids 101: Antibiotic Resistance Genes

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    ...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...

38. Antibodies 101: Epitope Tags

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    ... 8 amino acid tag (DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein...

39. Fluorescent Biosensors for Measuring Autophagic Flux

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    ...fluorescent protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy...

40. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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    ...synthetic vs. natural gene are essential and complementary tools in a time where biological engineering...

41. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    ... scientists fused them to sequences called complementarity determining regions (CDRs, or loops) of an ...

42. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    ...agreements slow down your research; provide specific examples if possible Tell them about the Golden Stopwatch...

43. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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    ...contribution to the community because of..." This first couple of sentences - even if written in a clumsy language...

44. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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    ... specific activity for each reagent batch. To supplement this, Addgene will be creating a crowdsourced...

45. Meet the New Addgene Board Members!

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    ...Medical School (HMS) where he leads a lab of 25 people. He also teaches at HMS and has been involved in...

46. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    ...might make it difficult to use NgAgo to disrupt multiple targets. Are you using NgAgo? Let us know how ...

47. Trading Spaces - Visiting Scientist Edition

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    ...and a priority list of experiments you hope to complete. Share this plan with your host prior to arrival...

48. K. phaffii: Rising to the Occasion

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    ...1990s when they abandoned the system. Since then, multiple biotech and pharmaceutical companies (along with...

49. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    ... validated the serotonin sensor with proof-of-principle experiments that replicate current knowledge, ...

50. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    ...multicolor reporters that will enable imaging of multiple proteases at once (Zhang et al., 2019). Studying...