...practical strategies for using combinations of AAV-based molecular tools to achieve targeted neuronal manipulation...

...this limitation, we created a generalizable tag-based strategy, which we refer to as the degradation tag...system is versatile and can be used in both cell-based assays and mouse models. The dTAG system The dTAG...

...mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in systems, these systems remain ...difficult. Addgene depositors have developed new CRISPR-based methods for various knock-ins, discussed in our ...

...your email or phone call. Though my experience is based solely on my time at Addgene, I’m confident that...minutes from our phone call. If your concern is based on data you have produced, please share the data...

...Nanoblades are a Cas9 RNP delivery vehicle that’s based on mouse leukemia virus (MLV) virus-like particles...Homology directed repair (HDR) with Nanoblades CRISPR-based HDR allows for the precise insertion of a DNA sequence...

... lentiGuide-based) Mammalian Lentivirus pRDA_722 (CMV-driven; lentiCRISPRv2-based; preferred ...

...specificity. Strength and accuracy The strength is based on binding specificity and exposure time. We first...: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ... dedicated to creating a public and accessible database for low-cost, high-quality antibodies for IHC....reference. The Gift of Giving As an open-source database, the mAb3D Atlas will foster collaboration, progress...reliable, high-quality antibodies. We are creating a database for goldilocks antibodies and, alongside that,...

...expand your search. Results in your feed are ranked based on their influence on the network, or body of literature...library. The library will suggest additional papers based on the papers you have saved. This library can also...

...illustrious speaker list attracted a host of sponsors based all over the world. With three Nobel Prize winners...hypothesis that these pathways are involved in RNA based surveillance of repetitive gene families and provide...

...or repressor domains to control gene expression. Base editors can help you make precise point mutations...consider the best form for those CRISPR components based on your model system. CRISPR reagents can be delivered...

...photos/72005145@N00/5600978712/ What CRISPR-based reagents are available for genome-wide screens? ...enriched using either drug selection or fluorescence-based cell sorting and screened for a particular phenotype...

...bench, with a special focus on cloning and plasmid-based technologies. Commentary about scientific sharing...

...and publish a human-designed protein that was not based on any existing protein structures, called Top7....

...is vital for the accurate interpretation of FRET-based experiments. A popular combination is Alexa594, ...dyes, expanding the capabilities of fluorescence-based techniques in biotechnology. Recent examples of ...2016). Development of a quantitative fluorescence-based ligand-binding assay. Scientific Reports, 6. https...

...event, and assigns each event to a channel number based on intensity. Higher intensity events are assigned...to the protein in its native state.  Antibodies-based flow cytometry can use both direct and indirect ... background information for setting up antibody based flow cytometry experiments.       References and...

...questions, which are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent...different city, you can still develop a successful FRET-based assay since energy transfer only occurs when donor...10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic...

...quickly and inexpensively generate a potent Cas9-based transcriptional regulator without making any functional...date his group has develop several popular CRISPR-based tools for altering DNA sequence or regulating gene...

... John Doench et al. shows that mutating a single base pair may not be enough to prevent re-binding. Lastly...region in a UTR, CrispyCrunch flips the two important base pairs of the PAM (NGG -> NCC, CCN -> GGN). This ... much as possible, it uses standard tools and databases, including primer3, biopython, bwa, samtools, ...

...but their initial results show promise for CRISPR-based gene activation. dCas9-CBP In this method, dCas9...2013) RNA-guided gene activation by CRISPR-Cas9–based transcription factors. Nat Methods 10:973–976 . ...

... electroporations. The specifics of this can be based on the manufacturer’s protocol for transformation...transformation efficiency, or more specifically calculated based on the size of and weight of your library plasmids...