We narrowed to 710 results for: BASE;
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TypeBlog Post...learn how to communicate their work differently based on their audience. How you talk about your experiments...problem was the creation of an Addgene lexicon. The base lexicon was and continues to be a list of commonly...
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Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...the Rare Genomics Institute by providing plasmid-based tools for rare disease research. Congratulations...events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors to monitor intracellular signaling... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog Post... modified) for optimal stability and efficacy. Base modifications What is more stable than RNA? DNA!...gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https... -
CRISPRainbow and Genome Visualization
TypeBlog Post...and sensitive enough to detect gene copy number based on the number of fluorescent puncta observed. By...Addgene Blog Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...barcoded gRNA library is now ready for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone...: PMC4420636. 7. Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...conclusions. Antibody engineering also benefits antibody-based therapeutics. Whether antibodies are used directly...measurement of antigen-antibody interactions in a plate-based format, making it suitable for comparing relative...biology, and protein engineering to develop antibody-based cancer therapeutics. References and Resources... -
Lentiviral Prep Service
TypeCollection...Lentivirus Browse our list of available viruses based on plasmids in our repository that express fluorescent... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post... lentiGuide-based) Mammalian Lentivirus pRDA_722 (CMV-driven; lentiCRISPRv2-based; preferred ... -
dTAG - You're it!
TypeBlog Post...this limitation, we created a generalizable tag-based strategy, which we refer to as the degradation tag...system is versatile and can be used in both cell-based assays and mouse models. The dTAG system The dTAG... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...Nanoblades are a Cas9 RNP delivery vehicle that’s based on mouse leukemia virus (MLV) virus-like particles...Homology directed repair (HDR) with Nanoblades CRISPR-based HDR allows for the precise insertion of a DNA sequence... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...expand your search. Results in your feed are ranked based on their influence on the network, or body of literature...library. The library will suggest additional papers based on the papers you have saved. This library can also... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...specificity. Strength and accuracy The strength is based on binding specificity and exposure time. We first...: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies ... dedicated to creating a public and accessible database for low-cost, high-quality antibodies for IHC....reference. The Gift of Giving As an open-source database, the mAb3D Atlas will foster collaboration, progress...reliable, high-quality antibodies. We are creating a database for goldilocks antibodies and, alongside that,... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in systems, these systems remain ...difficult. Addgene depositors have developed new CRISPR-based methods for various knock-ins, discussed in our ... -
Tips for Technical Support Calls
TypeBlog Post...your email or phone call. Though my experience is based solely on my time at Addgene, I’m confident that...minutes from our phone call. If your concern is based on data you have produced, please share the data... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...illustrious speaker list attracted a host of sponsors based all over the world. With three Nobel Prize winners...hypothesis that these pathways are involved in RNA based surveillance of repetitive gene families and provide... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...or repressor domains to control gene expression. Base editors can help you make precise point mutations...consider the best form for those CRISPR components based on your model system. CRISPR reagents can be delivered... -
Genome-wide Screening Using CRISPR
TypeBlog Post...photos/72005145@N00/5600978712/ What CRISPR-based reagents are available for genome-wide screens? ...enriched using either drug selection or fluorescence-based cell sorting and screened for a particular phenotype... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog Post...protocol management. Though this is a subscription-based software, Labguru does offer free trials and pricing... https://www.quartzy.com/ Quartzy is a free web-based lab management tool that allows labs to create accounts... -
Addgene’s New Way to Help You Share Science
TypeBlog Post...bench, with a special focus on cloning and plasmid-based technologies. Commentary about scientific sharing... -
Penn Vector Core Partnership with Addgene
TypeCollection... community. The Penn Vector Core produced viral-based vectors for distribution via Addgene. For a full...look up a viral vector with the Penn catalog ID: Based on low demand, production of some viral vectors ...still available, other serotypes may be available) Based on low demand, production of some viral vectors ...