We narrowed to 802 results for: Clu
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TypeBlog Post...number of unique features compared to Cas9. These include: More targeting flexibility Unlike Cas9 and the...base excision would alter the target sequence and preclude future HDR, which could lower HDR efficiency. ...
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Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post...napkin for paper, a small group of scientists including Stanley Falkow, Stanley Cohen, Herbert Boyer, ...eBook. Some highlights of the 2nd edition eBook include: new chapters on genome engineering, cloning, and... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...month. We are currently focused on two projects, including one led by a talented biomedical engineering team...still able to get through to a number of experts, including Harvard/MIT faculty, venture capital and angel... -
Another Pathway into Cells: iTOP
TypeBlog Post...the specificity of their luciferase assay, they concluded that components in the buffer used for protein...works in multiple types of primary mouse cells, including neuronal and gut stem cells, dendritic cells, ... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds that can recruit...RNA in the CRISPRi system can be repurposed to include modular RNA scaffolds. These RNA scaffolds can... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog Post...creative thinking Enhance leadership skills Support inclusion, diversity, and open communication Feel valued...download the "Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...Volunteer at a local high school science class: Science Club for Girls) Communication to diverse audiences...Communication Business models Conference or club organizer Management Leadership Communication... -
The MTA at Addgene
TypeBlog Post...understand and grow the scientific ecosystem, including understanding how shared materials are used to...ll be confident in what to expect. No system, including MTAs, is perfect, but it does provide consistency... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...libraries. NEB also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...researchers explore many aspects of ApoB-LP biology, including quantification of abundance, size, and localization...made these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as the eFRET sensors... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post... browse our entire AAV inventory. Our new AAVs include: Control AAVs for serotype testing jGCaMP7c for...best serotype for their particular study, which includes comparing the transduction patterns of different... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post...pivotal role in an array of biological processes including development, the immune system, and cell turnover...been difficult until now (Zhang et al., 2019). Conclusion FlipGFP is a novel fluorogenic reporter that ... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...of physical materials that we use in our labs, including plasmids. All plasmids at Addgene have unique ...materials and methods section have been updated to include the option of citing with resolvable compact identifier... -
Pushing the Limits of DNA Assembly
TypeBlog Post...are multiple methods of DNA assembly available, including: Gibson Assembly®, BioBrick® Assembly, Golden ...applications of the NEBuilder HiFi DNA Assembly Mix include: Generation of constructs with multiple point... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...oligomerization of the Dronpa domains thereby occluding the enzyme active site and shutting off the enzyme...of interest into its component simple sugars (including lactose), and a lac operon controlled GFP. When... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post... new contact to connect on LinkedIn and always include a personal note about where you met and what you...can be as simple as “it was nice meeting you.” Include follow-up questions or requests for advice based... -
DAP arrays for multiplex gene editing
TypeBlog Post..., across four different types of human cells, including T cells and hematopoietic stem and progenitor ... from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely on an upstream... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog Post...that can influence the HDR/NHEJ decision. Our conclusions reinforce a key scientific principle: that understanding...frequencies of genome cutting and, when donor DNA is included in the nucleofection reaction, extremely high ... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...plasmid on the right has a total size of 7.3kb, including a 1.2 kb insert. The plasmid was digested with...run on an agarose gel. The resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is...in the cell, as many of these experiments are concluded within 48 h of transfection. Of course, it is ...