We narrowed to 799 results for: Clu

...the specificity of their luciferase assay, they concluded that components in the buffer used for protein...works in multiple types of primary mouse cells, including neuronal and gut stem cells, dendritic cells, ...

...multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds that can recruit...RNA in the CRISPRi system can be repurposed to include modular RNA scaffolds. These RNA scaffolds can...

...creative thinking Enhance leadership skills Support inclusion, diversity, and open communication Feel valued...download the "Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the...

...Volunteer at a local high school science class: Science Club for Girls) Communication to diverse audiences...Communication Business models Conference or club organizer Management Leadership Communication...

...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...libraries. NEB also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated...

...are multiple methods of DNA assembly available, including: Gibson Assembly®, BioBrick® Assembly, Golden ...applications of the NEBuilder HiFi DNA Assembly Mix include:  Generation of constructs with multiple point...

...oligomerization of the Dronpa domains thereby occluding the enzyme active site and shutting off the enzyme...of interest into its component simple sugars (including lactose), and a lac operon controlled GFP. When...

...researchers explore many aspects of ApoB-LP biology, including quantification of abundance, size, and localization...made these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as the eFRET sensors...

... browse our entire AAV inventory. Our new AAVs include: Control AAVs for serotype testing jGCaMP7c for...best serotype for their particular study, which includes comparing the transduction patterns of different...

...pivotal role in an array of biological processes including development, the immune system, and cell turnover...been difficult until now (Zhang et al., 2019). Conclusion FlipGFP is a novel fluorogenic reporter that ...

...understand and grow the scientific ecosystem, including understanding how shared materials are used to...ll be confident in what to expect. No system, including MTAs, is perfect, but it does provide consistency...

... new contact to connect on LinkedIn and always include a personal note about where you met and what you...can be as simple as “it was nice meeting you.” Include follow-up questions or requests for advice based...

..., across four different types of human cells, including T cells and hematopoietic stem and progenitor ... from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely on an upstream...

...of physical materials that we use in our labs, including plasmids. All plasmids at Addgene have unique ...materials and methods section have been updated to include the option of citing with resolvable compact identifier...

...that can influence the HDR/NHEJ decision. Our conclusions reinforce a key scientific principle: that understanding...frequencies of genome cutting and, when donor DNA is included in the nucleofection reaction, extremely high ...

...plasmid on the right has a total size of 7.3kb, including a 1.2 kb insert. The plasmid was digested with...run on an agarose gel. The resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from...

...plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is...in the cell, as many of these experiments are concluded within 48 h of transfection. Of course, it is ...

...will it make them think? Does this lead to the conclusions I want them to draw and the perspective I want...scientists has formed around science communication, including the teams at Addgene, ComSciCon, and many more...

...Reis e Sousa from the Francis Crick Institute concluded “Aspirin promotes autophagy, inhibits platelet...field of Immuno-Oncology, a sizzling hot topic, includes studies on novel cancer therapeutics such as  ...

...information on each cancer clinical sequencing report includes rare variants that haven’t been characterized?...the scientific community through Addgene. These include 634 sequence confirmed mutant cDNA clones corresponding...