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We narrowed to 441 results for: DUR

Showing: 361 - 380 of 441 results

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CRISPR 101: Homology Directed Repair

Type

Blog Post

... fill in the resulting ssDNA. A single HJ forms during this process and resolves by cleavage of the crossed...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Type

Blog Post

... the cut site were not efficiently incorporated during HDR. We decided to systematically characterize ...
10 Steps to a Perfect Science Talk

Type

Blog Post

...always have a few extra slides in their back pocket. During your practice rounds, encourage your listeners ...
Site Directed Mutagenesis by PCR

Type

Blog Post

... successfully anneal to the plasmid of interest during the PCR reaction. Ideally, your primers should ...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...target sequence. Kaminski et al. next tested their procedure in HIV-1 infected T-cells to see if they could...
CRISPR 101: Off-Target Effects

Type

Blog Post

...which rely on insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific...
Plasmids 101: Dimers and Multimers

Type

Blog Post

...whole plasmid sequencing of our plasmid samples during our quality control process. Since MiSeq involves...
Plasmids 101: Repressible Promoters

Type

Blog Post

... ADH1 promoter pADH1m, which maintains activity during the late ethanol consumption phase of yeast culture...
When Fidelity Matters: A frank discussion about ligase fidelity

Type

Blog Post

... do with the slight melting of the 5 ́-terminus during the reaction. This “peeling back” of the 5 ́-phosphorylated...
A History of Genome Engineering in Popular Culture

Type

Blog Post

... sparked the imagination of most of the authors during this period. Genetic Engineering Era: Pre-CRISPR...
Plasmids 101: Using Transposons in the Lab

Type

Blog Post

...insertions were positive or negative selected for during the experiment (Sandoval-Villegas et al., 2021)...
Deep Dive: Statistical Tests (Comparisons)

Type

Blog Post

...Bonferroni Correction and the Benjamini-Hochberg procedure are designed to reduce false positive rates and...
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Type

Blog Post

... organisms which I use to study gene regulation during developmental processes, CRISPR/Cas9 allows us ...
With an Eye Towards the Future, We Look Back at the March for Science

Type

Blog Post

...evidence,” and to speak out in unprecedented force during the 2015 federal election. Even as we marched...
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

Type

Blog Post

...organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis." J Cell ...
Antibodies 101: Designing Your First Flow Panel

Type

Blog Post

...photon is emitted. This step is called emission. During excitation, some of the photon's energy is lost...
Plasmids 101: Modular Cloning Applications and Kits

Type

Blog Post

...without the undesirable side products generated during chemical synthesis of these molecules. To make ...
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Type

Blog Post

..., it is much easier to genetically modify cells during the reprogramming process as they are propagated...
CRISPR 101: Multiplex Expression of gRNAs

Type

Blog Post

... Note: depending on which method you use, the procedure will vary slightly. Two Golden Gate options...
How to Deposit Your Plasmids with Addgene

Type

Blog Post

... plasmid information. Enter plasmid information During the remainder of the process, you will enter plasmid...

Showing: 361 - 380 of 441 results

Results per page:

CRISPR 101: Homology Directed Repair

3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

10 Steps to a Perfect Science Talk

Site Directed Mutagenesis by PCR

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

CRISPR 101: Off-Target Effects

Plasmids 101: Dimers and Multimers

Plasmids 101: Repressible Promoters

When Fidelity Matters: A frank discussion about ligase fidelity

A History of Genome Engineering in Popular Culture

Plasmids 101: Using Transposons in the Lab

Deep Dive: Statistical Tests (Comparisons)

An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

With an Eye Towards the Future, We Look Back at the March for Science

Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

Antibodies 101: Designing Your First Flow Panel

Plasmids 101: Modular Cloning Applications and Kits

Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

CRISPR 101: Multiplex Expression of gRNAs

How to Deposit Your Plasmids with Addgene

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