We narrowed to 443 results for: DUR

1. Five Popular Model Organisms, Part 2

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   ...be analyzed to understand human disease alleles during and after embryogenesis. Addgene has over 600 plasmids...

2. CRISPR 101: Homology Directed Repair

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   ... fill in the resulting ssDNA. A single HJ forms during this process and resolves by cleavage of the crossed...

3. Antibodies 101: Introduction to Immunofluorescence

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   ...High-level overview of an indirect immunofluorescence procedure with key considerations about each step. Image...

4. The Fluorescent Vegetables in Aptamer Soup

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   ...Warner et al., 2014); its length reduces artefacts during live cell imaging. Bunch of baby spinach, or BoBS...

5. 10 Steps to a Perfect Science Talk

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   ...always have a few extra slides in their back pocket. During your practice rounds, encourage your listeners ...

6. Site Directed Mutagenesis by PCR

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   ... successfully anneal to the plasmid of interest during the PCR reaction. Ideally, your primers should ...

7. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   ...target sequence. Kaminski et al. next tested their procedure in HIV-1 infected T-cells to see if they could...

8. Hot Plasmids: Spring 2025

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   ...important modulators of cell signaling pathways during embryonic development and cancer. But there is ...

9. Plasmids 101: Dimers and Multimers

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   ...whole plasmid sequencing of our plasmid samples during our quality control process. Since MiSeq involves...

10. Plasmids 101: Repressible Promoters

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    ... ADH1 promoter pADH1m, which maintains activity during the late ethanol consumption phase of yeast culture...

11. CRISPR 101: Off-Target Effects

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    ...which rely on insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific...

12. When Fidelity Matters: A frank discussion about ligase fidelity

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    ... do with the slight melting of the 5 ́-terminus during the reaction. This “peeling back” of the 5 ́-phosphorylated...

13. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    ... organisms which I use to study gene regulation during developmental processes, CRISPR/Cas9 allows us ...

14. Deep Dive: Statistical Tests (Comparisons)

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    ...Bonferroni Correction and the Benjamini-Hochberg procedure are designed to reduce false positive rates and...

15. Plasmids 101: Using Transposons in the Lab

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    ...insertions were positive or negative selected for during the experiment (Sandoval-Villegas et al., 2021)...

16. A History of Genome Engineering in Popular Culture

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    ... sparked the imagination of most of the authors during this period.  Genetic Engineering Era: Pre-CRISPR...

17. Antibodies 101: Designing Your First Flow Panel

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    ...photon is emitted. This step is called emission. During excitation, some of the photon's energy is lost...

18. Plasmids 101: Modular Cloning Applications and Kits

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    ...without the undesirable side products generated during chemical synthesis of these molecules. To make ...

19. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    ...organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis." J Cell ...

20. With an Eye Towards the Future, We Look Back at the March for Science

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    ...evidence,” and to speak out in unprecedented force during the 2015 federal election.   Even as we marched...