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We narrowed to 768 results for: Dos

Showing: 361 - 380 of 768 results

  1. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ... scifi movie? Or maybe it’s a biosensor?! You can do more than just ‘sense’ biological compounds and reactions...activities.       Viruses and biosensors So how do viruses factor into all of these diverse biosensors...

  2. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...site specifically incorporated ncAA, ready for you do to any number of experiments with. The catch The...broadly useful for biomedical researchers. One way of doing that is by using Addgene’s Blue Flame award to identify...

  3. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ... enter the nucleus through nuclear pores and thus do not require breakdown of the nuclear envelope during...division to enter the nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus...

  4. The time and cost required to make a plasmid

    Type
    Blog Post
    .... That’s also a great way to get to know people doing interesting and relevant work in your field! However...single well in a 96-well (or 384-well) plate! How much do repositories help?   Designing precise calculations...

  5. Antibodies 101: Isotypes

    Type
    Blog Post
    ...is an isotype? How many are there? Why do they matter? And how do antibodies switch isotypes? What is an...

  6. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...your email for confirmation of submission. If you do not receive such a notice within 24 hrs, send a note...while you are climbing the academic ladder. If you do not get accepted to your top choice this year but...

  7. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...experiments. But how? There have been several systems to do this but most are labor-intensive, require multiple...

  9. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...fallen into studies of who’s there and what are they doing. To address these questions, biologists often use...transcripts gives clues into what the microbes are doing. But aside from sequencing, scientists can also ...

  10. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... time and budget you cannot test them all, so how do you know which ones to try?  Read on and we will ...turn to for advice if you need to troubleshoot. What do you know about the vendor and the vendor’s technical...

  13. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    .... Unlike cytoskeletal polymers, however, R bodies do not exert force by dynamically rearranging into different...bodies can be found at Addgene here. What can R bodies do for you? R bodies are robust linear actuators that...

  16. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ... the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are eukaryotes... Plasmids for use in S. pombe, on the other hand, do not require a well defined ORI. Instead, the size...

  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...’ll need to align all these images once taken. To do so, you will need to include a marker like the Hoechst...needs, your sample, and targets, you might decide to do simultaneous IF instead (Figure 1B). To label all...important consideration when deciding whether you can do simultaneous or sequential labeling is what conjugated...

  18. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...exciting trends in your experimental groups, but how do you determine if they deserve that oh-so coveted ...can be run on these tests. All sample comparisons Do you really need to know every pairwise comparison...mathematician to select the right statistical test, just do your homework and happy testing!   References Wissing...

  19. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...use, of course, depends on what you are trying to do – there’s no “best” tool among them. While this seems...gRNA depends an awful lot on what you are trying to do: gene knockout, a specific base edit, or modulation...importance in design – an optimized sequence will do little if it is in the wrong place, but because the...

  20. Deep Dive: qPCR

    Type
    Blog Post
    ... for your cell lines and/or tissue types.  Please do note my oh-so-subtle use of reference genes, plural...reference genes - and it is well worth your time to do one (Biassoni, 2014). At the bench As a highly sensitive...you from understanding how the analyses work - you do need to make sure the assumptions in your dataset...end of your analysis process. You’ll still need to do statistical analysis to determine if there are any...
Showing: 361 - 380 of 768 results