We narrowed to 772 results for: Dos
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TypeBlog Post...unexplored areas with our color-changing flower, but to do that we’ve had to navigate the challenges involved... plant synthetic biology. We cannot build what we do not understand, and the complicated and unique metabolic...
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The Twelve Days of Antibodies
TypeBlog Post... to our blog and social media. This year, we're doing so through the twelve days of antibodies. We hope... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...since most universities and non-profit organizations do not have the April 1 H-1B queue process or quota ... the performing arts- not relevant at all. Please do not include the fact that you are a classically trained...self-petition. You need the support of your institute to do this one. Universities will usually only support ...who could be beneficial during a career search. Doing these simple things will make it easier for you ... -
Plan Your Experiment
TypeGuide... model a specific disease or process of interest. Do you want to: Generate complete and permanent loss...target cells are not transcribing DNA to mRNA, nor do they need to translate mRNA to protein. Instead, ...a lack of efficient target cleavage in cells that do express both Cas9 and gRNA. Before choosing a validation...using cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA method described... -
Trends in CRISPR and SynBio Technologies
TypeBlog Post...areas of research. We welcome your comments... Where do you think these technologies are going? What is the... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...promoter region. Ok, I have a Cas9! What do i do next? Designing or selecting a sgRNA Addgene may... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post...together long enough to get it into cells and let them do the repair work. The key is T4 DNA polymerase, which...and its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless, ... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses...promoter is no longer present in these constructs. Why do many γ-retroviral plasmids have similar names? The... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...and a quick zap of the proper color light, you can do just that. Photosensitizing dyes and proteins have...than shorter wavelengths, which makes it possible to do things like in vivo imaging. Excitation with NIR... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...experimental conditions. This is time well spent and, if you do it right, can be (part of) figure 1 of your next ...reported properties of fluorescent proteins, how to do a head-to-head comparison of fluorescent proteins... -
Clear and Accessible Writing
TypeBlog Post...questions: What is the goal, who is my audience, and what do I want to be different for them when I’m done? What... Excellent! We asked our workshop participants to do the same thing, and even though only about 10% of... -
Viral Vectors 101: Biosensors
TypeBlog Post... scifi movie? Or maybe it’s a biosensor?! You can do more than just ‘sense’ biological compounds and reactions...activities. Viruses and biosensors So how do viruses factor into all of these diverse biosensors... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...site specifically incorporated ncAA, ready for you do to any number of experiments with. The catch The...broadly useful for biomedical researchers. One way of doing that is by using Addgene’s Blue Flame award to identify... -
Viral Vectors 101: Types of viruses
TypeBlog Post... enter the nucleus through nuclear pores and thus do not require breakdown of the nuclear envelope during...division to enter the nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus... -
The time and cost required to make a plasmid
TypeBlog Post.... That’s also a great way to get to know people doing interesting and relevant work in your field! However...single well in a 96-well (or 384-well) plate! How much do repositories help? Designing precise calculations... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post... time and budget you cannot test them all, so how do you know which ones to try? Read on and we will ...turn to for advice if you need to troubleshoot. What do you know about the vendor and the vendor’s technical... -
Antibodies 101: Isotypes
TypeBlog Post...is an isotype? How many are there? Why do they matter? And how do antibodies switch isotypes? What is an... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post...your email for confirmation of submission. If you do not receive such a notice within 24 hrs, send a note...while you are climbing the academic ladder. If you do not get accepted to your top choice this year but... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...experiments. But how? There have been several systems to do this but most are labor-intensive, require multiple... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...fallen into studies of who’s there and what are they doing. To address these questions, biologists often use...transcripts gives clues into what the microbes are doing. But aside from sequencing, scientists can also ...