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...promoter region.   Ok, I have a Cas9! What do i do next? Designing or selecting a sgRNA Addgene may...

...together long enough to get it into cells and let them do the repair work. The key is T4 DNA polymerase, which...and its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless, ...

...Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses...promoter is no longer present in these constructs. Why do many γ-retroviral plasmids have similar names? The...

...and a quick zap of the proper color light, you can do just that.  Photosensitizing dyes and proteins have...than shorter wavelengths, which makes it possible to do things like in vivo imaging.   Excitation with NIR...

...experimental conditions. This is time well spent and, if you do it right, can be (part of) figure 1 of your next ...reported properties of fluorescent proteins, how to do a head-to-head comparison of fluorescent proteins...

...fallen into studies of who’s there and what are they doing. To address these questions, biologists often use...transcripts gives clues into what the microbes are doing. But aside from sequencing, scientists can also ...

...questions: What is the goal, who is my audience, and what do I want to be different for them when I’m done? What... Excellent! We asked our workshop participants to do the same thing, and even though only about 10% of...

... scifi movie? Or maybe it’s a biosensor?! You can do more than just ‘sense’ biological compounds and reactions...activities.       Viruses and biosensors So how do viruses factor into all of these diverse biosensors...

...site specifically incorporated ncAA, ready for you do to any number of experiments with. The catch The...broadly useful for biomedical researchers. One way of doing that is by using Addgene’s Blue Flame award to identify...

... enter the nucleus through nuclear pores and thus do not require breakdown of the nuclear envelope during...division to enter the nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus...

.... That’s also a great way to get to know people doing interesting and relevant work in your field! However...single well in a 96-well (or 384-well) plate! How much do repositories help?   Designing precise calculations...

...your email for confirmation of submission. If you do not receive such a notice within 24 hrs, send a note...while you are climbing the academic ladder. If you do not get accepted to your top choice this year but...

... time and budget you cannot test them all, so how do you know which ones to try?  Read on and we will ...turn to for advice if you need to troubleshoot. What do you know about the vendor and the vendor’s technical...

...is an isotype? How many are there? Why do they matter? And how do antibodies switch isotypes? What is an...

...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...experiments. But how? There have been several systems to do this but most are labor-intensive, require multiple...

.... Unlike cytoskeletal polymers, however, R bodies do not exert force by dynamically rearranging into different...bodies can be found at Addgene here. What can R bodies do for you? R bodies are robust linear actuators that...

...each one and make sure it’s working. You can only do that screen with a system you are really confident...depending on which guide RNA we use. What we have to do now is learn more about the rules.” He said that ...

...to tell a story - convince your audience you are doing amazing things."  As Sample6 raised more money, ...seeking funding, ask yourself if you really want to do this.” Biotech startups are only successful if they...

... the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are eukaryotes... Plasmids for use in S. pombe, on the other hand, do not require a well defined ORI. Instead, the size...

...’ll need to align all these images once taken. To do so, you will need to include a marker like the Hoechst...needs, your sample, and targets, you might decide to do simultaneous IF instead (Figure 1B). To label all...important consideration when deciding whether you can do simultaneous or sequential labeling is what conjugated...