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We narrowed to 610 results for: GAN

Showing: 361 - 380 of 610 results

  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add a co-factor...mTurquoise2, not shown) remains fluorescent in acidic organelles (Figure 1), showing that its acid tolerance is...

  2. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...

  3. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...interference by double-stranded RNA in Caenorhabditis elegans. Nature, 391(6669), 806–811. https://doi.org/10.1038.... https://doi.org/10.1007/978-1-60761-657-3_10 Vergani-Junior, C. A., Tonon-Da-Silva, G., Inan, M. D.,...

  4. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022). The oxytocin sensor MTRIAOT ...various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP to urotensin. The MTRIA...

  5. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...

  6. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more successful...Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K, Hu W. CRISPR...

  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ..., B. K., Aoi, Y., Iwanaszko, M., Howard, B. C., Morgan, M. A., Andersen, G. D., Bartom, E. T., & Shilatifard...Efficient proximity labeling in living cells and organisms with TurboID. Nature Biotechnology, 36(9), 880...

  9. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly...

  10. Finding Your Perfect Job After University

    Type
    Blog Post
    ...Luckily, I have worked for a variety of different organizations, so I tend to provide them with an overview ...practices, but can sometimes go unnoticed at large organizations. Another downside to large commercial companies...

  12. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...skilled at presenting and answering questions. Organizing a thoughtful engaging talk takes significant ...successful writers about their strategies for organizing and self-critiquing their own writings. 8. Learn...

  14. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...cells or tissue slices mounted to a slide, or whole organs. Because the tissue architecture is preserved, ...from the bloodstream. Then, via a blood draw or organ harvest, the labeled cells can be analyzed using...

  15. Typing CRISPR Systems

    Type
    Blog Post
    ...classification While CRISPR systems now seem neatly organized into their respective categories, arriving at ...similar levels. It was only recently that researchers began building optimal plasmids to harness the power of...

  17. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...with Addgene Senior Scientist, Matt Ferenc, to organize the CRISPR forum's most popular questions and ...https://doi.org/10.1038/nbt.2842  Soldner, F., Laganière, J., Cheng, A. W., Hockemeyer, D., Gao, Q., Alagappan...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...acquired from the the fluorescent and luminescent siganls and a ratio is calculated (the BRET ratio), which...including subcellular visualization of 6 different organelles, a cell cycle indicator for mouse embryonic stem...monitoring cargo trafficking, and for discovering organelle contact sites. This suite of deposited fluorescent...

  19. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...Dimers and Multimers" post. It's also been re-organized and updated with a fresh new look.  We think this...
Showing: 361 - 380 of 610 results