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We narrowed to 614 results for: GAN

Showing: 361 - 380 of 614 results

  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add a co-factor...mTurquoise2, not shown) remains fluorescent in acidic organelles (Figure 1), showing that its acid tolerance is...

  2. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...interference by double-stranded RNA in Caenorhabditis elegans. Nature, 391(6669), 806–811. https://doi.org/10.1038.... https://doi.org/10.1007/978-1-60761-657-3_10 Vergani-Junior, C. A., Tonon-Da-Silva, G., Inan, M. D.,...

  3. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...

  4. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022). The oxytocin sensor MTRIAOT ...various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP to urotensin. The MTRIA...

  5. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...

  6. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more successful...Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K, Hu W. CRISPR...

  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ..., B. K., Aoi, Y., Iwanaszko, M., Howard, B. C., Morgan, M. A., Andersen, G. D., Bartom, E. T., & Shilatifard...Efficient proximity labeling in living cells and organisms with TurboID. Nature Biotechnology, 36(9), 880...

  9. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly...

  10. Finding Your Perfect Job After University

    Type
    Blog Post
    ...Luckily, I have worked for a variety of different organizations, so I tend to provide them with an overview ...practices, but can sometimes go unnoticed at large organizations. Another downside to large commercial companies...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...lentiCRISPR v2-sgCOQ2 COQ2 U6 Multiple system atrophy Boyi Gan 186221 pBMN BFP-Parkin PRKN BFP Parkinson's Michael...Frontotemporal dementia in GRN mutation carriers Michele Pagano 210366 TMEM106B-EGFP TMEM106B GFP CMV, T7 Frontotemporal...Frontotemporal dementia in GRN mutation carriers Michele Pagano 210899 pFBOH-MHL_LRRK2:2141-2527 LRRK2 His polH...pFastBac-GST-TBK1 TBK1 His, TEV, GST polH ALS Jonathan Kagan 221522 SYNJ1_BirA_Cterm SYNJ1 BirA, Flag CMV Parkinsonism...

  13. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...skilled at presenting and answering questions. Organizing a thoughtful engaging talk takes significant ...successful writers about their strategies for organizing and self-critiquing their own writings. 8. Learn...

  15. Typing CRISPR Systems

    Type
    Blog Post
    ...classification While CRISPR systems now seem neatly organized into their respective categories, arriving at ...similar levels. It was only recently that researchers began building optimal plasmids to harness the power of...

  16. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...cells or tissue slices mounted to a slide, or whole organs. Because the tissue architecture is preserved, ...from the bloodstream. Then, via a blood draw or organ harvest, the labeled cells can be analyzed using...

  17. Luciferase Plasmid Collection

    Type
    Collection
    ...responsible for bioluminescence found in a variety of organisms, ranging from bacteria to aquatic animals to insects...

  18. Caltech Systemic Capsids

    Type
    Collection
    ...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...

  20. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...with Addgene Senior Scientist, Matt Ferenc, to organize the CRISPR forum's most popular questions and ...https://doi.org/10.1038/nbt.2842  Soldner, F., Laganière, J., Cheng, A. W., Hockemeyer, D., Gao, Q., Alagappan...
Showing: 361 - 380 of 614 results