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We narrowed to 765 results for: Mos

Showing: 361 - 380 of 765 results

  1. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ... Sanger sequencing of pooled samples showed that most of the HIV-1 genome had been excised, leaving only...rather than the ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR HIV-1 therapy...therapeutic possibilities for some of the world’s most common diseases. References Zhang Y, Yin C, Zhang...

  2. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ... Equity and inclusion The final and possibly the most important panel of the symposium was on diversity...easily digestible/available. What I viewed as the most important take-away from this session was the idea...are making great progress on some of the world’s most profound questions, panelists and participants of...

  3. CRISPR Guide

    Type
    Guide
    ...Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo, H., Zhou, R., Lang, B., Meng, B., Weng, H., & Hu...inversions with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to target...simplicity and adaptability, CRISPR rapidly became the most popular genome engineering approach. Figure 1: Overview...ease of generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR...mutations (see: Validate genetic modification ). In most cases, NHEJ gives rise to small indels in the target...homology elsewhere in the genome. Realistically, most gRNA targeting sequences will have additional sites...specificity. SpCas9 (from Streptococcus pyogenes ) is the most popular Cas endonuclease, with many modified versions...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...phosphoproteins Protein phosphorylation is one of the most abundant forms of post-translational modifications...Classic genetic engineering methods enabling chromosomal integration of sequences in bacteria are time-consuming...frequently, so visit our CRISPR pages often to find the most recently deposited tools, resources, protocols, ... popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies in Addgene's First...

  5. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...fact, we usually recommend testing more than one.  Most AAV serotypes have broad tropism (the ability of...promoters  These often-strong promoters offer the most flexibility for expression in a large number of ...adeno-associated viral vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex. Neuroscience...

  6. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...deactivation rates By Mike LacyGlutamate is one of the most important and abundant neurotransmitters, but it...membrane display, they recommend the NGR vectors for most experiments. These new tools promise to be a popular...By Meghan Rego You asked, we listened! One of the most powerful tools in a scientist's toolkit is the empty...

  7. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...viruses that prey on bacteria) may be the most numerous and most diverse biological entities on our planet...recognition factors of phages) constitute one of the most popular classes of phage proteins to study. These...

  10. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ... used. Inducible lentiviral transfer vectors The most widely used inducible lentiviral vector system is...this, you can use the transfection reagent you are most comfortable with. If you don’t have any experience...depends on the type of cell you would like to infect. Most of the protocols you will find will recommend using...

  11. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... Specialist in Patrick Hsu's lab at UC Berkeley. Most of the scientists I’ve met began doing research ...epidemiology, public health, and more.     A few of the most common roles that you’ll see in a lab. Position...dedicate your time and energy to. How can I make the most of my experience in the lab? Ask your questions—...

  13. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...the number of cells needed is lower, and perhaps most important, targeting all genes doesn’t necessarily...group works on immune cells in different contexts. Most of the immune cells we study are migratory, and ...candidate genes. Green Listed, as of now, contains most of the full genome knockout human and mouse libraries...

  14. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...where excitation and emission are the most efficient (i.e., happen most of the time). Figure 1 shows a graph...molecule), their bleed is so strong that these spectra almost overlap. Hence, they should not be used within ...

  15. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...same chemical reaction performed by CBEs. However, most CBEs include an inhibitor of base excision repair... a C → G editing efficiency of up to 68% at the most favorable target sites. Differential cytosine editing...on to develop more efficient and precise editors. Most researchers agreed on the first part of the likely...

  16. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...the CTD (also known as the PI), while REC includes most of the bridge helix. Created with BioRender.com....conformation frequently (Osuka et al., 2018), but it’s most often shown in an “open” state, with the NUC and...region is disordered, Cas9 binds DNA very weakly, most likely through unstructured electrostatic interactions...

  18. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...alternative approach is RNA interference (RNAi). The most common method is transfection of short interfering...protein), which has been designated CD365 in the most recent HLDA workshop (HLDA10), as an example. For...cell lines using these two antibodies. This is the most usual level of evidence supplied to support the ...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...such as malaria and toxoplasmosis. Despite their importance to global health, most apicomplexan genes ...bidirectional promoter, integrate at 11 specific chromosomal locations, and stably express the integrated ...are derived from the Ralser lab's own pHLUM minichromosomal vector, and besides being used for restoring...

  20. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar más emocionados! Después de recibir la traducción...científica profesional, L. Denise Roche, para editarla. Estamos orgullosos de anunciar que CRISPR 101: Una herramienta...
Showing: 361 - 380 of 765 results