...positive cells (Martin et al., 2019). Figure 2: Editing efficiencies using base editing reporters...by canonical base excision repair enzymes. At the same time Cas9n cleaves the opposing DNA strain which...

...to the break room for a sip of water.  Fig 2: Blugene likes to spark creativity in out on the open...you and you will arrive at unique solutions to the same problem. If your lab/company/team is still working...

...gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) General protein expression...-deficient; allows for cloning and expression in same strain F- recA1 hsdR(rK12- mK12+) (DE3) (RifR) ...

... PMID: 9537413. PubMed Central PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating...require modifying multiple genes simultaneously in the same cell, are more appropriate for a CRISPR-based approach...

... Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion Don’t just plan... it perfectly fits the occasion.  I speak on the same topics many times, but I always seek out information...

...Postdoctoral Associations around Boston - From Panel Session 2: Defining the Postdoc: An Institutional Perspective...underrepresented groups under their mentorship with the same critical eye they place on others within their labs...

...improvement in muscle physiological performance.  2. Trans-splicing Overview: For trans-plicing, splice...nbt0702-697 Hirsch ML, Agbandje-McKenna M, Jude Samulski R (2010) Little Vector, Big Gene Transduction:...Bellon I, Yin C, Chavala S, Pryadkina M, Richard I, Samulski RJ (2013) Oversized AAV Transductifon Is Mediated...

...variety of environmental conditions, and double every 2 hours. Yeast are also the first eukaryotic genome ... model organism in scientific research. Yes, the same kind we use in breads and other baked goods! Yeast...

..., whereas the mVenus-APT1 fusion does not (figure 2). In the mVenus-APT1, a lipidation motif, which is...conclusive evidence that the fusion operates in the same way as the native protein. Although a fluorescent...

...Nutr, 129 (2009a): 477–484. PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic...pre-incubated with free avidin can be added to your sample. Any free biotin-binding sites on this complex ...

... more from the authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression...to the range of concentrations expected in your samples. Higher affinity isn’t always better, especially...

... iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d ..., and deep penetration of red-shifted light. The same NIR FPs and biosensors can be imaged at spatial ...

...15.4%) 46 (59%) of these students were in the last 2 years of their degree. Students filled in a pre-program... my mentor, since she does research at UK in the same field that I do: prostate cancer. She has guided...

...biology 14.6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure...Watson-Crick base pair, are joined with nearly the same efficiency as a correct base pair. DNA ligases have... resistance-mediating polymorphisms in African samples of Plasmodium falciparum." Journal of clinical ...

...experience in cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in...cancer and I loved the practical aspects of testing samples in the lab, but I really struggled with the large...

...effective recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced....changes 5’ to the PAM, sequences corresponding to the same strand as the PAM produced a higher editing efficiency...

...indicated, the antibiotic powder can be dissolved in dH 2 0. Addgene recommends making 1000X stock solutions...daughter cells. These daughter cells contain the same genetic information as the parental cell, and are...

... 10 90 2 x 10 8 10 of 2 x 10 8 dilution 90 2 x 10 7 10 of 2 x 10 7 dilution 90 2 x 10 6 10 of 2 x 10 6...6 dilution 90 2 x 10 5 10 of 2 x 10 5 dilution 90 2 x 10 4 10 of 2 x 10 4 dilution 90 2 x 10 3 Pro-Tip...molecules/μL To obtain a solution at 2 x 10 9 molecules/μL: 1.59 x 10 11 / 2 x 10 9 = 79.8X dilution ...your standard curve plasmid (2 x 10 9 stock made in step #1): Volume of 2 x 10 9 stock or previous dilution...stock 45 uL 10X 10X Dilution 2 5 uL Dil. 1 95 uL 20X 200X Dilution 3 20 uL Dil. 2 80 uL 5X 1000X Dilution ...empty NTC C AAV reference Sample 3 D E Sample 1 Sample 4 F E Sample 2 Sample 5 F Perform data analysis...virion). 5 μL sample + 39 μL H 2 O + 5 μL 10X DNase buffer + 1 μL DNase Gently mix sample (do not vortex...

...phosphate monobasic monohydrate (NaH 2 PO 4 ∙H 2 O), pH 7.0 138 g NaH 2 PO 4 ∙H 2 O 1 L deionized water Adjust...sodium phosphate dibasic (NaH 2 PO 4 ), pH 7.0 142 g of NaH 2 PO 4 ∙H 2 O 1 L deionized water Adjust pH...monobasic monohydrate (NaH 2 PO 4 ∙H 2 O) 610 mL of sterile sodium phosphate dibasic (NaH 2 PO 4 ) Store up to...purifying the same recombinant antibody. Section 2: Buffer exchange Choose Option 1 or Option 2 based on the...antibody sample. Pro-Tip If the volume of the sample is greater than 4 mL , then divide it into 2 columns...concentration of the pooled sample is below 1.0 mg/mL proceed to Option 2 with a buffer exchange/concentration...Centrifuge at 1000 x g for 2 min to collect the sample. Discard column after use. Determine antibody concentration...

...430825, 175 cm 2 Cellstack 5, Corning 3319, 3180 cm 2 Cellstack 2, Corning 3269, 1272 cm 2 Heat-inactivated...: 50 mM Tris HCl, 150 mM NaCl, 2 mM MgCl 2 Add the following to the 2 L sterile bottle: 1836 mL deionized... solution can be stored at 4 °C for up to 2 months. After 2 months, discard the tube and thaw a new working... mL of PBS. Aspirate PBS and add 2 mL of 0.05% Trypsin/EDTA. Wait ~2 min. Neutralize trypsin by adding...(CS5) (Link opens in a new window) (3,180 cm 2 - the same surface area as 21 x T-175 flasks). Cell stacks...flasks. Workflow Timeline Day 0: Seed cells in CS2 Day 2: Seed cells in CS5 Day 3 (am): Transfect cells Day...Biological Safety Cabinet 0.5–10 µL single channel pipette 2–20 µL single channel pipette 20–200 µL single channel...