We narrowed to 763 results for: SCR

...developing a new imaging-based bacterial colony screening platform, Oliver Griesbeck’s lab set out to engineer...colleagues started with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and tested a ...

...DNA repair (with bonus gRNA design resources.) Subscribe to CRISPR updates on our blog to keep current ...Chris Richardson and members of Jacob Corn’s lab describe how a better understanding of Cas9 biophysics ...

...generally refer to them as the “interested public.” Subscribers to Scientific America would be considered the...scientists in (roughly) the same field, often through manuscripts, seminars, or posters. It is important to note...

...ready-to-use viral preps   Improved cell-free transcription/translation system to examine biosynthetic pathways...pathways  By Gabrielle Clouse Recently, cell-free transcription/translation systems (also referred to as TX-...

...protein of interest. 4. Plasmids 101: Blue-White Screening Getting a lot of empty vector contaminants in ...Addgenie Jessica Welch explains how Blue-White screening could be a potential solution. 5. Cpf1: A New ...

...as their kidneys fail. The condition was first described more than 60 years ago, but there is still no ...changes in temperature.  “The disease was first described in 1971,” McSweeney said. “But, it is still sometimes...

...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...

...Coordinator’ came up at Abcam. Unlike other job descriptions for marketing positions posted on recruitment...would be able to carry out a yeast-2-hybrid library screen; instead I thought they would focus on “Has this...

...published in Nature Methods, Boyden and his team describe the discovery of a light-sensitive opsin from ...then, there has been a steady stream of papers describing new photosynthetic or photosensory molecules ...

...disease severity. The MECP2 protein is a global transcriptional regulator of thousands of genes and studies...studies have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...DNA and recruit known co-repressors or other transcriptional modifiers suggests the major function of MECP2...NTD) the M ethyl B inding D omain (MBD) a T ranscriptional R epressor D omain (TRD) the TRD contains a...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...Developing Human Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH...

...Other Applications Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused...Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into a specific...transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9 fused to an activator..., or fused to a repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier...

...H134R)-EYFP Flp Yes 126080 pAAV-Ef1a-sCreDIO hChR2(H134R)-eYFP Scre No 126081 pAAV-Ef1a-vCreDIO hChR2(H134R...public folder (Link opens in a new window) of manuscripts that have used these constructs experimentally...Yes 55638 pAAV-EF1a-vCre None Yes 55635 pAAV-EF1a-sCre None No 55633 pAAV-EF1a-mCherry-IRES-Dre None No...in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing Neurons in ...in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1...

...are also thought to play a key role in the transcriptional regulation of many genes. A number of groups...thus far for these latter experiments, such as transcription factors tethered to dCas9, require long time...

...promoter regions that activate fluorescent protein transcription in response to river contaminants and transform...Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for updates and future collaborations...

...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...scientific communities, but usually I only get to scratch the surface of different fields. This meeting has...

...that lab notebook from 2 years ago. The free subscription to Lab Archives allots for 25 MB of space per...only need to skim the alphabet on the side of the screen to find the sequence for your enzyme of interest...

...mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact that coiled R-...both lower and higher pH. We initially used this screen to find R body mutants that couldn’t extend at ...

...directly transformed into E.coli for plating, screening and generation of sgRNA. Generation of sgRNA libraries...Assembly and Other Long-Homology Based Cloning Methods Screen Plasmids by Colony PCR Addtional Resources on ...

...phrase gram negative or positive being used to describe a species of bacteria, but what does it actually...What is a plasmid? Plasmids 101: Blue-white screeningPlasmids 101: Common lab E. Coli strains  ...

... knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm presents an attractive...! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol...