We narrowed to 767 results for: SCR

...allows you to fuse proteins of interest to transcriptional regulatory elements that will turn on gene ...and an engineered strain for a B2H system used to screen the activity of arrays of zinc finger nucleases...Propionate Escherichia coli Jay Keasling 113634 pSCrhaB2 PrhaB Rhamnose Burkholderia cenocepacia Miguel...nm) Escherichia coli Christopher Voigt 164226 pSCrhaB2plus PrhaBAD Rhamnose Burkholderia sp. Silvia Cardona...selection of plasmids using tetracycline-regulated transcriptional activation or repression. The BIOFAB plasmid...that element. When this activation requires a transcription factor to bind to a small molecule, the reporter...DNA-binding specificity of proteins such as transcription factors. Addgene Blog Luciferase Technologies...

...developing a new imaging-based bacterial colony screening platform, Oliver Griesbeck’s lab set out to engineer...colleagues started with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and tested a ...

...DNA repair (with bonus gRNA design resources.) Subscribe to CRISPR updates on our blog to keep current ...Chris Richardson and members of Jacob Corn’s lab describe how a better understanding of Cas9 biophysics ...

...your institution’s tech transfer professionals: Describe to your TTO how unprocessed agreements slow down...University  Salk Institute for Biological Studies The Scripps Research Institute St. Jude Children's Research...

...tetramer-breaking mutations. The library was then screened through successive rounds of mutagenesis for the...choosing fluorescent proteins for your research Subscribe to the Addgene blog Resources on Addgene.org ...

...multicolor labeling is a powerful solution for discriminating between neurons for light microscopy-based ...expression level of XFPs, most likely by suppressing transcription. It is critical to express a minimal amount ...

...generally refer to them as the “interested public.” Subscribers to Scientific America would be considered the...scientists in (roughly) the same field, often through manuscripts, seminars, or posters. It is important to note...

...ready-to-use viral preps   Improved cell-free transcription/translation system to examine biosynthetic pathways...pathways  By Gabrielle Clouse Recently, cell-free transcription/translation systems (also referred to as TX-...

...protein of interest. 4. Plasmids 101: Blue-White Screening Getting a lot of empty vector contaminants in ...Addgenie Jessica Welch explains how Blue-White screening could be a potential solution. 5. Cpf1: A New ...

...as their kidneys fail. The condition was first described more than 60 years ago, but there is still no ...changes in temperature.  “The disease was first described in 1971,” McSweeney said. “But, it is still sometimes...

...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...

...Coordinator’ came up at Abcam. Unlike other job descriptions for marketing positions posted on recruitment...would be able to carry out a yeast-2-hybrid library screen; instead I thought they would focus on “Has this...

...published in Nature Methods, Boyden and his team describe the discovery of a light-sensitive opsin from ...then, there has been a steady stream of papers describing new photosynthetic or photosensory molecules ...

...directly transformed into E.coli for plating, screening and generation of sgRNA. Generation of sgRNA libraries...Assembly and Other Long-Homology Based Cloning Methods Screen Plasmids by Colony PCR Addtional Resources on ...

...are also thought to play a key role in the transcriptional regulation of many genes. A number of groups...thus far for these latter experiments, such as transcription factors tethered to dCas9, require long time...

...phrase gram negative or positive being used to describe a species of bacteria, but what does it actually...What is a plasmid? Plasmids 101: Blue-white screeningPlasmids 101: Common lab E. Coli strains  ...

... knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm presents an attractive...! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol...

...promoter regions that activate fluorescent protein transcription in response to river contaminants and transform...Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for updates and future collaborations...

...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...scientific communities, but usually I only get to scratch the surface of different fields. This meeting has...

...that lab notebook from 2 years ago. The free subscription to Lab Archives allots for 25 MB of space per...only need to skim the alphabet on the side of the screen to find the sequence for your enzyme of interest...